Ribosomal RNA large subunit methyltransferase E (P0C0R7)

Identification
Name:Ribosomal RNA large subunit methyltransferase E
Synonyms:
  • 23S rRNA Um2252 methyltransferase
  • Cell division protein ftsJ
  • rRNA (uridine-2'-O-)-methyltransferase
Gene Name:rlmE
Enzyme Class:
Biological Properties
General Function:Translation, ribosomal structure and biogenesis
Specific Function:Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
GO Classification:
Function
binding
catalytic activity
methyltransferase activity
nucleic acid binding
RNA methyltransferase activity
rRNA (uridine) methyltransferase activity
rRNA methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
cellular metabolic process
macromolecule metabolic process
metabolic process
methylation
ncRNA metabolic process
one-carbon metabolic process
RNA metabolic process
rRNA metabolic process
rRNA methylation
rRNA modification
rRNA processing
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>630 bp
ATGACAGGTAAGAAGCGTTCTGCCAGCTCCAGCCGCTGGCTTCAGGAACACTTTAGCGAT
AAATATGTTCAACAGGCACAGAAAAAGGGGTTACGTTCCCGTGCCTGGTTTAAACTTGAT
GAAATACAGCAAAGTGACAAACTCTTTAAACCGGGAATGACGGTTGTCGACCTTGGTGCT
GCTCCGGGTGGTTGGTCACAATATGTGGTCACCCAAATTGGCGGCAAAGGCCGCATCATC
GCTTGCGATCTTTTACCTATGGATCCTATCGTTGGTGTGGACTTTCTTCAGGGCGATTTT
CGTGATGAACTGGTGATGAAAGCACTGCTGGAGCGCGTTGGCGACAGCAAAGTCCAGGTT
GTCATGTCCGATATGGCACCAAACATGAGCGGAACACCGGCGGTGGATATCCCCCGTGCC
ATGTATCTGGTGGAACTGGCGCTAGAAATGTGTCGTGATGTATTAGCGCCAGGTGGCAGT
TTTGTAGTGAAGGTGTTCCAGGGCGAAGGTTTCGATGAGTATCTAAGGGAAATTCGCTCC
CTGTTTACGAAGGTCAAAGTTCGTAAGCCGGACTCTTCTCGTGCACGTTCGCGGGAAGTG
TATATTGTAGCGACCGGGCGTAAACCCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:209
Protein Molecular Weight:23335
Protein Theoretical pI:10
PDB File:1EJ0
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal RNA large subunit methyltransferase E
MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQSDKLFKPGMTVVDLGA
APGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRDELVMKALLERVGDSKVQV
VMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGSFVVKVFQGEGFDEYLREIRS
LFTKVKVRKPDSSRARSREVYIVATGRKP
References
External Links:
ResourceLink
Uniprot ID:P0C0R7
Uniprot Name:RLME_ECOLI
GenBank Gene ID:M83138
Genebank Protein ID:146027
PDB ID:1EJ0
CCDB:RLME_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bugl, H., Fauman, E. B., Staker, B. L., Zheng, F., Kushner, S. R., Saper, M. A., Bardwell, J. C., Jakob, U. (2000). "RNA methylation under heat shock control." Mol Cell 6:349-360. Pubmed: 10983982
  • Caldas, T., Binet, E., Bouloc, P., Costa, A., Desgres, J., Richarme, G. (2000). "The FtsJ/RrmJ heat shock protein of Escherichia coli is a 23 S ribosomal RNA methyltransferase." J Biol Chem 275:16414-16419. Pubmed: 10748051
  • Caldas, T., Binet, E., Bouloc, P., Richarme, G. (2000). "Translational defects of Escherichia coli mutants deficient in the Um(2552) 23S ribosomal RNA methyltransferase RrmJ/FTSJ." Biochem Biophys Res Commun 271:714-718. Pubmed: 10814528
  • Hager, J., Staker, B. L., Bugl, H., Jakob, U. (2002). "Active site in RrmJ, a heat shock-induced methyltransferase." J Biol Chem 277:41978-41986. Pubmed: 12181314
  • Hager, J., Staker, B. L., Jakob, U. (2004). "Substrate binding analysis of the 23S rRNA methyltransferase RrmJ." J Bacteriol 186:6634-6642. Pubmed: 15375145
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Tan, J., Jakob, U., Bardwell, J. C. (2002). "Overexpression of two different GTPases rescues a null mutation in a heat-induced rRNA methyltransferase." J Bacteriol 184:2692-2698. Pubmed: 11976298
  • Tomoyasu, T., Yuki, T., Morimura, S., Mori, H., Yamanaka, K., Niki, H., Hiraga, S., Ogura, T. (1993). "The Escherichia coli FtsH protein is a prokaryotic member of a protein family of putative ATPases involved in membrane functions, cell cycle control, and gene expression." J Bacteriol 175:1344-1351. Pubmed: 8444796