ECMDB

Identification

Name:

Acyl-CoA thioesterase 2

Synonyms:

Acyl-CoA thioesterase II
TEII

Gene Name:

tesB

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in acyl-CoA hydrolase activity

Specific Function:

Can hydrolyze a broad range of acyl-CoA thioesters. Its physiological function is not known

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Not Available

KEGG Pathways:

Biosynthesis of unsaturated fatty acids ec01040

SMPDB Reactions:

1.0trans-Octadec-2-enoyl-CoA

1.0

→

1.0

1.0Elaidic acid

1.0trans-Octadec-2-enoyl-CoA + 1.0Water + 1.0Trans-Octadec-2-enoyl-CoA → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0Elaidic acid
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Water + 1.0Octanoyl-CoA → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0Caprylic acid
ReactionCard

1.0

→

1.0

1.0Water + 1.0Palmityl-CoA → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0Palmitic acid
ReactionCard

1.0

→

1.0

1.0Water + 1.0Tetradecanoyl-CoA → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0tetradecanoate (n-C14:0)
ReactionCard

1.0

→

1.0

1.0Water + 1.0Hexanoyl-CoA → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0Hexanoate (N-C6:0)
ReactionCard

1.0

→

1.0

1.0Water + 1.0(2E)-Tetradecenoyl-CoA → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0Tetradecenoate (N-C14:1)
ReactionCard

1.0

→

1.0

1.0Water + 1.0Octadecenoyl-CoA (N-C18:1CoA) → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0Octadecenoate (N-C18:1)
ReactionCard

1.0

→

1.0

1.0Water + 1.0Stearoyl-CoA → 1.0Coenzyme A + 1.0Hydrogen ion + 1.0Octadecanoate (N-C18:0)
ReactionCard

1.0

→

1.0

1.0Lauroyl-CoA + 1.0Water → 1.0Coenzyme A + 1.0Dodecanoate (N-C12:0) + 1.0Hydrogen ion
ReactionCard

1.0

→

1.0

1.0Decanoyl-CoA (N-C10:0CoA) + 1.0Water → 1.0Coenzyme A + 1.0Decanoate (N-C10:0) + 1.0Hydrogen ion
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB03945	(2E)-Hexadecenoyl-CoA	MetaboCard
ECMDB03946	(2E)-Tetradecenoyl-CoA	MetaboCard
ECMDB00482	Caprylic acid	MetaboCard
ECMDB01423	Coenzyme A	MetaboCard
ECMDB21204	Decanoate (N-C10:0)	MetaboCard
ECMDB06404	Decanoyl-CoA (N-C10:0CoA)	MetaboCard
ECMDB21209	Dodecanoate (N-C12:0)	MetaboCard
ECMDB21229	Hexanoate (N-C6:0)	MetaboCard
ECMDB02845	Hexanoyl-CoA	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB03571	Lauroyl-CoA	MetaboCard
ECMDB21347	Octadecanoate (N-C18:0)	MetaboCard
ECMDB21348	Octadecenoate (N-C18:1)	MetaboCard
ECMDB21260	Octadecenoyl-CoA (N-C18:1CoA)	MetaboCard
ECMDB01070	Octanoyl-CoA	MetaboCard
ECMDB00220	Palmitic acid	MetaboCard
ECMDB01338	Palmityl-CoA	MetaboCard
ECMDB21284	Stearoyl-CoA	MetaboCard
ECMDB21361	tetradecanoate (n-C14:0)	MetaboCard
ECMDB01521	Tetradecanoyl-CoA	MetaboCard
ECMDB21362	Tetradecenoate (N-C14:1)	MetaboCard
ECMDB21363	Trans-Octadec-2-enoyl-CoA	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
acyl-CoA thioesterase activity
catalytic activity
CoA hydrolase activity
hydrolase activity
hydrolase activity, acting on ester bonds
thiolester hydrolase activity
Process
acyl-CoA metabolic process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process

Gene Properties

Blattner:

b0452

Gene Orientation

Counterclockwise

Centisome Percentage:

10.21

Left Sequence End

473525

Right Sequence End

474385

Gene Sequence:

>861 bp
ATGAGTCAGGCGCTAAAAAATTTACTGACATTGTTAAATCTGGAAAAAATTGAGGAAGGA
CTCTTTCGCGGCCAGAGTGAAGATTTAGGTTTACGCCAGGTGTTTGGCGGCCAGGTCGTG
GGTCAGGCCTTGTATGCTGCAAAAGAGACCGTCCCTGAAGAGCGGCTGGTACATTCGTTT
CACAGCTACTTTCTTCGCCCTGGCGATAGTAAGAAGCCGATTATTTATGATGTCGAAACG
CTGCGTGACGGTAACAGCTTCAGCGCCCGCCGGGTTGCTGCTATTCAAAACGGCAAACCG
ATTTTTTATATGACTGCCTCTTTCCAGGCACCAGAAGCGGGTTTCGAACATCAAAAAACA
ATGCCGTCCGCGCCAGCGCCTGATGGCCTCCCTTCGGAAACGCAAATCGCCCAATCGCTG
GCGCACCTGCTGCCGCCAGTGCTGAAAGATAAATTCATCTGCGATCGTCCGCTGGAAGTC
CGTCCGGTGGAGTTTCATAACCCACTGAAAGGTCACGTCGCAGAACCACATCGTCAGGTG
TGGATCCGCGCAAATGGTAGCGTGCCGGATGACCTGCGCGTTCATCAGTATCTGCTCGGT
TACGCTTCTGATCTTAACTTCCTGCCGGTAGCTCTACAGCCGCACGGCATCGGTTTTCTC
GAACCGGGGATTCAGATTGCCACCATTGACCATTCCATGTGGTTCCATCGCCCGTTTAAT
TTGAATGAATGGCTGCTGTATAGCGTGGAGAGCACCTCGGCGTCCAGCGCACGTGGCTTT
GTGCGCGGTGAGTTTTATACCCAAGACGGCGTACTGGTTGCCTCGACCGTTCAGGAAGGG
GTGATGCGTAATCACAATTAA

Protein Properties

Pfam Domain Function:

Acyl_CoA_thio (PF02551 )

Protein Residues:

286

Protein Molecular Weight:

31966

Protein Theoretical pI:

PDB File:

1C8U

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Acyl-CoA thioesterase 2
MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQALYAAKETVPEERLVHSF
HSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIFYMTASFQAPEAGFEHQKT
MPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEVRPVEFHNPLKGHVAEPHRQV
WIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIGFLEPGIQIATIDHSMWFHRPFN
LNEWLLYSVESTSASSARGFVRGEFYTQDGVLVASTVQEGVMRNHN

References

External Links:

Resource	Link
Uniprot ID:	P0AGG2
Uniprot Name:	TESB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674592
PDB ID:	1C8U
Ecogene ID:	EG10995
Ecocyc:	EG10995
ColiBase:	b0452
Kegg Gene:	b0452
EchoBASE ID:	EB0988
CCDB:	TESB_ECOLI
BacMap:	16128437

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Li, J., Derewenda, U., Dauter, Z., Smith, S., Derewenda, Z. S. (2000). "Crystal structure of the Escherichia coli thioesterase II, a homolog of the human Nef binding enzyme." Nat Struct Biol 7:555-559. Pubmed: 10876240
Naggert, J., Narasimhan, M. L., DeVeaux, L., Cho, H., Randhawa, Z. I., Cronan, J. E. Jr, Green, B. N., Smith, S. (1991). "Cloning, sequencing, and characterization of Escherichia coli thioesterase II." J Biol Chem 266:11044-11050. Pubmed: 1645722

Acyl-CoA thioesterase 2 (P0AGG2)