Dihydrofolate reductase folM (P0AFS3)

Identification
Name:Dihydrofolate reductase folM
Synonyms:
  • DHFR
Gene Name:folM
Enzyme Class:
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Catalyzes the reduction of dihydrofolate to tetrahydrofolate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Dihydrofolic acid + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0NADP + 1.0Tetrahydrofolic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Tetrahydrofolic acid + 1.0NAD ↔ 1.0Dihydrofolic acid + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Tetrahydrofolic acid + 1.0NADP ↔ 1.0Dihydrofolic acid + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.07,8-dihydrofolate monoglutamate+1.0Thumb+1.0NADPH+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Tetrahydrofolic acid+1.0Thumb
1.07,8-dihydrofolate monoglutamate + 1.0Hydrogen ion + 1.0NADPH + 1.0Dihydrofolic acid + 1.0NADPH → 1.0NADP + 1.0Tetrahydrofolic acid + 1.0Tetrahydrofolic acid
ReactionCard
1.0Dihydromethysticin+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Dihydromethysticin + 1.0NADPH + 1.0Hydrogen ion → 1.0Tetrahydromonapterin + 1.0NADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Dihydrofolic acid + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0NADP + 1.0Tetrahydrofolic acid
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.07,8-Dihydroneopterin + 1.0Hydrogen ion + 1.0NADPH → 1.0NADP + 1.0Tetrahydromonapterin
ReactionCard
1.0Thumb+1.0Thumb ← 1.0Thumb+1.0Thumb+1.0Thumb
1.0NADP + 1.0Tetrahydrofolic acid ← 1.0Hydrogen ion + 1.0NADPH + 1.0Dihydrofolic acid
ReactionCard
1.0Thumb+1.0Thumb ← 1.0Thumb+1.0Thumb+1.0Thumb
1.0Tetrahydromonapterin + 1.0NADP ← 1.0Hydrogen ion + 1.07,8-Dihydroneopterin + 1.0NADPH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB022757,8-DihydroneopterinMetaboCard
ECMDB01056Dihydrofolic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB01846Tetrahydrofolic acidMetaboCard
ECMDB21288TetrahydromonapterinMetaboCard
GO Classification:
Function
binding
catalytic activity
oxidoreductase activity
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b1606
Gene OrientationClockwise
Centisome Percentage:36.19
Left Sequence End1679000
Right Sequence End1679722
Gene Sequence:
>723 bp
ATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCC
TGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATG
TATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGC
GCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCCGTCCAGGTACAGGCGGAC
CCACTTCATCTTCCTTTTGCCGATAAATCCGTTGATGTTTGTCTACTGGCACATACATTG
CCGTGGTGCACCGATCCGCATCGTTTATTGCGTGAAGCCGATCGGGTATTGATTGATGAT
GGCTGGCTGGTCATTAGTGGCTTCAATCCCATCAGTTTTATGGGATTACGCAAACTTGTG
CCGGTATTGCGCAAAACCTCGCCCTATAACAGCCGGATGTTTACTCTGATGCGGCAGCTG
GACTGGCTCTCTTTGTTGAATTTTGAAGTGCTACACGCCAGCCGTTTCCACGTTCTCCCG
TGGAACAAACACGGAGGAAAACTATTGAATGCGCATATTCCTGCGCTTGGTTGCTTACAA
CTTATTGTTGCCCGGAAACGGACTATTCCTTTAACGCTAAATCCGATGAAACAGAGTAAA
AACAAGCCACGAATTCGCCAGGCGGTTGGAGCCACCCGGCAATGTCGTAAACCACAGGCT
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:240
Protein Molecular Weight:26348
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Dihydrofolate reductase folM
MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLINAGAQCIQADFS
TNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVLACMMQIHVNTPYLLNHAL
ERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDNMTRSFARKLAPEVKVNSIA
PSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSFPLDGGRHLR
References
External Links:
ResourceLink
Uniprot ID:P0AFS3
Uniprot Name:FOLM_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902950
Ecogene ID:EG13189
Ecocyc:EG13189
ColiBase:b1606
Kegg Gene:b1606
EchoBASE ID:EB2981
CCDB:FOLM_ECOLI
BacMap:16129564
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Giladi, M., Altman-Price, N., Levin, I., Levy, L., Mevarech, M. (2003). "FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli." J Bacteriol 185:7015-7018. Pubmed: 14617668
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553