Na(+)/H(+) antiporter nhaB (P0AFA7)

Identification
Name:Na(+)/H(+) antiporter nhaB
Synonyms:
  • Sodium/proton antiporter nhaB
Gene Name:nhaB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in sodium:hydrogen antiporter activity
Specific Function:Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons. Catalyzes the exchange of 3 H(+) per 2 Na(+). Has a high affinity for sodium, but can also transport lithium. Activity is weakly pH-dependent. Essential for regulation of intracellular pH under alkaline conditions
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB02908SodiumMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
cation transmembrane transporter activity
hydrogen ion transmembrane transporter activity
inorganic cation transmembrane transporter activity
ion transmembrane transporter activity
monovalent cation:hydrogen antiporter activity
monovalent inorganic cation transmembrane transporter activity
sodium:hydrogen antiporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
monovalent inorganic cation transport
sodium ion transport
transport
Gene Properties
Blattner:b1186
Gene OrientationCounterclockwise
Centisome Percentage:26.56
Left Sequence End1232399
Right Sequence End1233940
Gene Sequence:
>1542 bp
ATGGAGATCTCCTGGGGCCGCGCGCTATGGCGCAATTTTTTGGGCCAGTCCCCCGACTGG
TACAAACTCGCCCTCATCATTTTCTTAATCGTAAACCCGTTAATTTTCCTCATCAGCCCT
TTCGTCGCGGGCTGGTTGCTGGTAGCGGAATTTATTTTCACTCTGGCGATGGCCCTGAAA
TGCTACCCGCTGCTCCCCGGTGGTCTGTTGGCTATCGAAGCGGTATTCATCGGCATGACC
AGCGCGGAACACGTCCGTGAAGAGGTGGCGGCAAATCTTGAAGTCTTGCTGTTACTGATG
TTTATGGTGGCGGGTATCTATTTTATGAAACAGCTGTTGCTGTTCATATTTACCCGTTTG
CTGTTAAGCATTCGCTCCAAAATGCTGCTGTCGCTCTCTTTTTGCGTGGCGGCTGCGTTC
CTCTCCGCGTTCCTCGATGCCTTAACCGTCGTGGCGGTGGTGATCAGCGTTGCAGTCGGT
TTTTATGGTATTTATCACCGCGTAGCCTCTTCCCGTACCGAAGACACCGACCTGCAAGAC
GATAGTCATATCGACAAGCATTACAAAGTGGTTCTGGAACAGTTCCGTGGCTTTCTGCGT
AGCCTGATGATGCATGCCGGTGTCGGCACCGCATTAGGCGGCGTAATGACCATGGTGGGC
GAACCACAGAACCTGATCATCGCTAAAGCGGCTGGCTGGCATTTTGGCGATTTCTTCCTG
CGCATGTCGCCGGTGACCGTTCCGGTTCTGATTTGTGGCCTGTTAACCTGCCTGCTGGTA
GAGAAGCTGCGTTGGTTTGGCTACGGTGAAACGCTGCCGGAGAAAGTCCGCGAAGTGTTG
CAACAGTTTGACGATCAAAGCCGCCACCAGCGTACCCGTCAGGATAAAATCCGTCTGATT
GTCCAGGCGATTATTGGCGTCTGGCTGGTGACTGCGCTGGCGTTGCATCTGGCGGAAGTT
GGCTTGATTGGTTTGTCAGTCATTATTCTGGCAACATCATTGACCGGTGTCACCGATGAG
CATGCTATCGGTAAAGCCTTCACCGAATCTCTGCCATTCACCGCACTGTTGACGGTGTTT
TTCTCGGTAGTCGCGGTGATTATCGACCAACAACTGTTTTCGCCAATTATTCAGTTTGTG
TTGCAGGCATCGGAACATGCTCAGCTGTCGCTGTTCTATATTTTCAACGGTCTGCTGTCA
TCCATTTCGGATAACGTCTTCGTGGGGACGATTTATATCAACGAAGCGAAAGCGGCAATG
GAAAGTGGCGCTATCACGTTGAAGCAATACGAGCTGCTGGCGGTCGCCATTAATACCGGT
ACCAATCTGCCCTCCGTCGCTACGCCGAACGGTCAGGCTGCGTTCCTGTTCCTGCTGACC
TCTGCACTCGCGCCATTGATTCGCCTCTCTTATGGCCGCATGGTGTGGATGGCCCTGCCT
TACACCCTCGTCCTGACACTCGTCGGCTTGCTCTGCGTCGAGTTTACGCTTGCCCCTGTA
ACCGAATGGTTTATGCAAATGGGCTGGATAGCAACGCTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:513
Protein Molecular Weight:56728
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • 23-43
  • 52-72
  • 97-117
  • 120-140
  • 144-164
  • 202-222
  • 238-258
  • 303-323
  • 348-368
  • 391-411
  • 447-467
  • 475-495
Protein Sequence:
>Na(+)/H(+) antiporter nhaB
MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAGWLLVAEFIFTLAMALK
CYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFMVAGIYFMKQLLLFIFTRL
LLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVGFYGIYHRVASSRTEDTDLQD
DSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKAAGWHFGDFFL
RMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKVREVLQQFDDQSRHQRTRQDKIRLI
VQAIIGVWLVTALALHLAEVGLIGLSVIILATSLTGVTDEHAIGKAFTESLPFTALLTVF
FSVVAVIIDQQLFSPIIQFVLQASEHAQLSLFYIFNGLLSSISDNVFVGTIYINEAKAAM
ESGAITLKQYELLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWMALP
YTLVLTLVGLLCVEFTLAPVTEWFMQMGWIATL
References
External Links:
ResourceLink
Uniprot ID:P0AFA7
Uniprot Name:NHAB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651583
Ecogene ID:EG11392
Ecocyc:EG11392
ColiBase:b1186
Kegg Gene:b1186
EchoBASE ID:EB1365
CCDB:NHAB_ECOLI
BacMap:16129149
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Inaba, K., Kuroda, T., Shimamoto, T., Kayahara, T., Tsuda, M., Tsuchiya, T. (1994). "Lithium toxicity and Na+(Li+)/H+ antiporter in Escherichia coli." Biol Pharm Bull 17:395-398. Pubmed: 8019504
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Pinner, E., Kotler, Y., Padan, E., Schuldiner, S. (1993). "Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli." J Biol Chem 268:1729-1734. Pubmed: 8093613
  • Pinner, E., Padan, E., Schuldiner, S. (1992). "Cloning, sequencing, and expression of the nhaB gene, encoding a Na+/H+ antiporter in Escherichia coli." J Biol Chem 267:11064-11068. Pubmed: 1317851
  • Pinner, E., Padan, E., Schuldiner, S. (1994). "Kinetic properties of NhaB, a Na+/H+ antiporter from Escherichia coli." J Biol Chem 269:26274-26279. Pubmed: 7929345
  • Shimamoto, T., Inaba, K., Thelen, P., Ishikawa, T., Goldberg, E. B., Tsuda, M., Tsuchiya, T. (1994). "The NhaB Na+/H+ antiporter is essential for intracellular pH regulation under alkaline conditions in Escherichia coli." J Biochem 116:285-290. Pubmed: 7822245