Glycerol uptake facilitator protein (P0AER0)

Identification
Name:Glycerol uptake facilitator protein
Synonyms:
  • Aquaglyceroporin
Gene Name:glpF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
  • glycerol metabolism PW000914
  • glycerol metabolism II PW000915
  • glycerol metabolism III (sn-glycero-3-phosphoethanolamine) PW000916
  • glycerol metabolism IV (glycerophosphoglycerol) PW000917
  • glycerol metabolism V (glycerophosphoserine) PW000918
  • inner membrane transport PW000786
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB01051D-GlyceraldehydeMetaboCard
ECMDB00131GlycerolMetaboCard
ECMDB04172UreaMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
transporter activity
Process
establishment of localization
transmembrane transport
transport
Gene Properties
Blattner:b3927
Gene OrientationCounterclockwise
Centisome Percentage:88.70
Left Sequence End4115268
Right Sequence End4116113
Gene Sequence:
>846 bp
ATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTT
ACCTTTCTCTACGCGCCAATGCTGATGCTGGTTATCTATTCGTTTAACAGCTCGAAGCTG
GTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCG
ATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCG
ATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT
GGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCG
CTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATG
CTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCT
TCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACG
CCGCTGAAAGTGTTTTTCGTCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGC
TGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGG
CCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCCG
GAAATCAACGCCCTGGCAACATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCC
TGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGT
GGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:281
Protein Molecular Weight:29780
Protein Theoretical pI:7
PDB File:1LDI
Signaling Regions:
  • None
Transmembrane Regions:
  • 6-34
  • 40-60
  • 85-108
  • 144-169
  • 178-194
  • 232-254
Protein Sequence:
>Glycerol uptake facilitator protein
MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISVIWGLGVAMAIYLTA
GVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAALVYGLYYNLFFDFEQTHH
IVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILMGLILALTDDGNGVPRGPLAP
LLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAGWGNVAFTGGRDIPYFLVPLFGP
IVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSEQKASL
References
External Links:
ResourceLink
Uniprot ID:P0AER0
Uniprot Name:GLPF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651390
PDB ID:1LDI
Ecogene ID:EG10396
Ecocyc:EG10396
ColiBase:b3927
Kegg Gene:b3927
EchoBASE ID:EB0391
CCDB:GLPF_ECOLI
BacMap:16131765
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borgnia, M. J., Agre, P. (2001). "Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli." Proc Natl Acad Sci U S A 98:2888-2893. Pubmed: 11226336
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Fu, D., Libson, A., Miercke, L. J., Weitzman, C., Nollert, P., Krucinski, J., Stroud, R. M. (2000). "Structure of a glycerol-conducting channel and the basis for its selectivity." Science 290:481-486. Pubmed: 11039922
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Heller, K. B., Lin, E. C., Wilson, T. H. (1980). "Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli." J Bacteriol 144:274-278. Pubmed: 6998951
  • Muramatsu, S., Mizuno, T. (1989). "Nucleotide sequence of the region encompassing the glpKF operon and its upstream region containing a bent DNA sequence of Escherichia coli." Nucleic Acids Res 17:4378. Pubmed: 2544860
  • Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018
  • Tajkhorshid, E., Nollert, P., Jensen, M. O., Miercke, L. J., O'Connell, J., Stroud, R. M., Schulten, K. (2002). "Control of the selectivity of the aquaporin water channel family by global orientational tuning." Science 296:525-530. Pubmed: 11964478
  • Unger, V. M. (2000). "Fraternal twins: AQP1 and GlpF." Nat Struct Biol 7:1082-1084. Pubmed: 11101882
  • Weissenborn, D. L., Wittekindt, N., Larson, T. J. (1992). "Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12." J Biol Chem 267:6122-6131. Pubmed: 1372899