Identification
Name:3-oxoacyl-[acyl-carrier-protein] reductase
Synonyms:
  • 3-ketoacyl-acyl carrier protein reductase
Gene Name:fabG
Enzyme Class:
Biological Properties
General Function:Involved in 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
Specific Function:(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0(3R)-3-Hydroxybutanoyl-[acyl-carrier protein]+1.0Thumb1.0Acetoacetyl-[acp]+1.0Thumb+1.0Thumb
1.0(3R)-3-Hydroxybutanoyl-[acyl-carrier protein] + 1.0NADP ↔ 1.0Acetoacetyl-[acp] + 1.0NADPH + 1.0Hydrogen ion
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1.0(3R)-3-Hydroxydecanoyl-[acyl-carrier protein]+1.0Thumb1.03-Oxodecanoyl-[acp]+1.0Thumb
1.0(3R)-3-Hydroxydecanoyl-[acyl-carrier protein] + 1.0NADP ↔ 1.03-Oxodecanoyl-[acp] + 1.0NADPH
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1.0(3R)-3-Hydroxyoctanoyl-[acyl-carrier protein]+1.0Thumb1.03-Oxooctanoyl-[acp]+1.0Thumb+1.0Thumb
1.0(3R)-3-Hydroxyoctanoyl-[acyl-carrier protein] + 1.0NADP ↔ 1.03-Oxooctanoyl-[acp] + 1.0NADPH + 1.0Hydrogen ion
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1.0(3R)-3-Hydroxypalmitoyl-[acyl-carrier protein]+1.0Thumb1.03-Oxohexadecanoyl-[acp]+1.0Thumb+1.0Thumb
1.0(3R)-3-Hydroxypalmitoyl-[acyl-carrier protein] + 1.0NADP ↔ 1.03-Oxohexadecanoyl-[acp] + 1.0NADPH + 1.0Hydrogen ion
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1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein]+1.0Thumb1.03-Oxotetradecanoyl-[acp]+1.0Thumb+1.0Thumb
1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] + 1.0NADP ↔ 1.03-Oxotetradecanoyl-[acp] + 1.0NADPH + 1.0Hydrogen ion
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1.0(R)-3-Hydroxyhexanoyl-[acp]+1.0Thumb1.03-Oxohexanoyl-[acp]+1.0Thumb+1.0Thumb
1.0(R)-3-Hydroxyhexanoyl-[acp] + 1.0NADP ↔ 1.03-Oxohexanoyl-[acp] + 1.0NADPH + 1.0Hydrogen ion
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1.0(R)-3-Hydroxydodecanoyl-[acp]+1.0Thumb1.03-Oxododecanoyl-[acp]+1.0Thumb+1.0Thumb
1.0(R)-3-Hydroxydodecanoyl-[acp] + 1.0NADP ↔ 1.03-Oxododecanoyl-[acp] + 1.0NADPH + 1.0Hydrogen ion
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1.0Thumb+1.0Thumb+1.0Thumb1.03-Hydroxyoctadecanoyl-CoA+1.0Thumb
1.03-Oxooctadecanoyl-CoA + 1.0NADPH + 1.0Hydrogen ion ↔ 1.03-Hydroxyoctadecanoyl-CoA + 1.0NADP
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1.03-Oxostearoyl-[acp]+1.0Thumb+1.0Thumb1.03-Hydroxyoctadecanoyl-[acp]+1.0Thumb
1.03-Oxostearoyl-[acp] + 1.0NADPH + 1.0Hydrogen ion ↔ 1.03-Hydroxyoctadecanoyl-[acp] + 1.0NADP
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1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
SMPDB Reactions:
1.03-Ketoglutaryl-[acp] methyl ester +1.0NADPH+1.0Thumb+1.0Thumb1.0Thumb+1.0a 3R-hydroxyglutaryl-[acp] methyl ester
1.03-Ketoglutaryl-[acp] methyl ester + 1.0NADPH + 1.0Hydrogen ion + 1.0NADPH → 1.0NADP + 1.0a 3R-hydroxyglutaryl-[acp] methyl ester
ReactionCard
1.03-Ketopimeloyl-[acp] methyl ester+1.0NADPH+1.0Thumb+1.0Thumb1.0Thumb+1.03-Hydroxypimeloyl-[acp] methyl ester
1.03-Ketopimeloyl-[acp] methyl ester + 1.0NADPH + 1.0Hydrogen ion + 1.0NADPH → 1.0NADP + 1.03-Hydroxypimeloyl-[acp] methyl ester
ReactionCard
1.03-oxo-cis-Δ5-dodecenoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.03R-hydroxy cis Δ9-hexadecenoyl-[acp] +1.0Thumb
1.03-oxo-cis-Δ5-dodecenoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.03R-hydroxy cis Δ9-hexadecenoyl-[acp] + 1.0NADP
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1.0a 3-oxo-cis-Δ7-tetradecenoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0a 3R-hydroxy cis Δ7-tetradecenoyl-[acp]+1.0Thumb
1.0a 3-oxo-cis-Δ7-tetradecenoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0a 3R-hydroxy cis Δ7-tetradecenoyl-[acp] + 1.0NADP
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1.03-oxo-cis-Δ9-hexadecenoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.03R-hydroxy cis Δ9-hexadecenoyl-[acp]
1.03-oxo-cis-Δ9-hexadecenoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.03R-hydroxy cis Δ9-hexadecenoyl-[acp]
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1.03-oxo-cis-vaccenoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.0(R)-3-hydroxy-cis-vaccenoyl-[acp]
1.03-oxo-cis-vaccenoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0(R)-3-hydroxy-cis-vaccenoyl-[acp]
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1.03-oxoacyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.0(3R)-3-hydroxyacyl-[acyl-carrier protein]
1.03-oxoacyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0(3R)-3-hydroxyacyl-[acyl-carrier protein]
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1.0acetoacetyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.0(R)-3-hydroxybutanoyl-[acp]
1.0acetoacetyl-[acp] + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0(R)-3-hydroxybutanoyl-[acp]
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1.03-oxo-hexanoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0(R)-3-hydroxyhexanoyl-[acp] +1.0Thumb
1.03-oxo-hexanoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0(R)-3-hydroxyhexanoyl-[acp] + 1.0NADP
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1.03-oxo-octanoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.0(R)-3-hydroxyoctanoyl-[acp]
1.03-oxo-octanoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0(R)-3-hydroxyoctanoyl-[acp]
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1.03-oxo-decanoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.0an (R)-3-hydroxydecanoyl-[acp] 
1.03-oxo-decanoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0an (R)-3-hydroxydecanoyl-[acp] 
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1.03-oxo-dodecanoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.0(R)-3-hydroxydodecanoyl-[acp]
1.03-oxo-dodecanoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0(R)-3-hydroxydodecanoyl-[acp]
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1.03-oxo-myristoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.0(3R)-3-hydroxymyristoyl-[acp]
1.03-oxo-myristoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0(3R)-3-hydroxymyristoyl-[acp]
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1.03-oxo-palmitoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.0(R)-3-hydroxypalmitoyl-[acp]
1.03-oxo-palmitoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0(R)-3-hydroxypalmitoyl-[acp]
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1.03-oxo-cis-Δ5-dodecenoyl-[acp] +1.0Thumb+1.0NADPH+1.0Thumb1.0Thumb+1.0a (3R)-hydroxy cis Δ5-dodecenoyl-[acp]
1.03-oxo-cis-Δ5-dodecenoyl-[acp]  + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0a (3R)-hydroxy cis Δ5-dodecenoyl-[acp]
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Complex Reactions:
3.0-oxo-cis-dodec-5-enoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-hydroxy-cis-dodec-5-enoyl-[acyl-carrier protein]+1.0Thumb
3.0-oxo-cis-dodec-5-enoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH → 1.0(R)-3-hydroxy-cis-dodec-5-enoyl-[acyl-carrier protein] + 1.0NADP
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3.0-oxo-cis-myristol-7-eoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-hydroxy-cis-myristol-7-eoyl-[acyl-carrier protein]+1.0Thumb
3.0-oxo-cis-myristol-7-eoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH → 1.0(R)-3-hydroxy-cis-myristol-7-eoyl-[acyl-carrier protein] + 1.0NADP
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3.0-oxo-cis-palm-9-eoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-hydroxy-cis-palm-9-eoyl-[acyl-carrier protein]+1.0Thumb
3.0-oxo-cis-palm-9-eoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH → 1.0(R)-3-hydroxy-cis-palm-9-eoyl-[acyl-carrier protein] + 1.0NADP
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3.0-oxo-cis-vacc-11-enoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-hydroxy-cis-vacc-11-enoyl-[acyl-carrier protein]+1.0Thumb
3.0-oxo-cis-vacc-11-enoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH → 1.0(R)-3-hydroxy-cis-vacc-11-enoyl-[acyl-carrier protein] + 1.0NADP
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3.0-Oxodecanoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-Hydroxydecanoyl-[acyl-carrier protein]+1.0Thumb
3.0-Oxodecanoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0(R)-3-Hydroxydecanoyl-[acyl-carrier protein] + 1.0NADP
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3.0-Oxododecanoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-Hydroxydodecanoyl-[acyl-carrier protein]+1.0Thumb
3.0-Oxododecanoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0(R)-3-Hydroxydodecanoyl-[acyl-carrier protein] + 1.0NADP
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3.0-Oxohexadecanoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0R-3-hydroxypalmitoyl-[acyl-carrier protein]+1.0Thumb
3.0-Oxohexadecanoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0R-3-hydroxypalmitoyl-[acyl-carrier protein] + 1.0NADP
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3.0-Oxohexanoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-Hydroxyhexanoyl-[acyl-carrier protein]+1.0Thumb
3.0-Oxohexanoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0(R)-3-Hydroxyhexanoyl-[acyl-carrier protein] + 1.0NADP
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3.0-Oxooctadecanoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-Hydroxyoctadecanoyl-[acyl-carrier protein]+1.0Thumb
3.0-Oxooctadecanoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0(R)-3-Hydroxyoctadecanoyl-[acyl-carrier protein] + 1.0NADP
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3.0-Oxooctanoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-Hydroxyoctanoyl-[acyl-carrier protein]+1.0Thumb
3.0-Oxooctanoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0(R)-3-Hydroxyoctanoyl-[acyl-carrier protein] + 1.0NADP
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3.0-Oxotetradecanoyl-[acyl-carrier protein]+1.0Thumb+1.0Thumb1.0(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein]+1.0Thumb
3.0-Oxotetradecanoyl-[acyl-carrier protein] + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] + 1.0NADP
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1.0Acetoacetyl-ACP+1.0Thumb+1.0Thumb1.0(3R)-3-Hydroxyacyl-[acyl-carrier protein]+1.0Thumb
1.0Acetoacetyl-ACP + 1.0Hydrogen ion + 1.0NADPH ↔ 1.0(3R)-3-Hydroxyacyl-[acyl-carrier protein] + 1.0NADP
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1.0Thumb+1.0Thumb+3.0-Oxo-glutaryl-[acyl-carrier protein] methyl ester3.0-Hydroxyglutaryl-[acyl-carrier protein] methyl ester+1.0Thumb
1.0Hydrogen ion + 1.0NADPH + 3.0-Oxo-glutaryl-[acyl-carrier protein] methyl ester → 3.0-Hydroxyglutaryl-[acyl-carrier protein] methyl ester + 1.0NADP
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1.0Thumb+1.0Thumb+3.0-Oxo-pimeloyl-[acyl-carrier protein] methyl ester3.0-Hydroxypimeloyl-[acyl-carrier protein] methyl ester+1.0Thumb
1.0Hydrogen ion + 1.0NADPH + 3.0-Oxo-pimeloyl-[acyl-carrier protein] methyl ester → 3.0-Hydroxypimeloyl-[acyl-carrier protein] methyl ester + 1.0NADP
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1.0(3R)-3-hydroxyacyl-[acyl-carrier-protein]+1.0Thumb1.03-oxoacyl-[acyl-carrier-protein]+1.0Thumb
1.0(3R)-3-hydroxyacyl-[acyl-carrier-protein] + 1.0NADP → 1.03-oxoacyl-[acyl-carrier-protein] + 1.0NADPH
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211703-Oxooctadecanoyl-CoAMetaboCard
ECMDB21538Heptadecenoic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21506Margaric acidMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
GO Classification:
Function
3-oxoacyl-[acyl-carrier-protein] reductase activity
acyltransferase activity
binding
catalytic activity
fatty acid synthase activity
NAD or NADH binding
nucleotide binding
oxidoreductase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
carboxylic acid metabolic process
cellular metabolic process
fatty acid biosynthetic process
fatty acid metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxidation reduction
oxoacid metabolic process
Gene Properties
Blattner:b1093
Gene OrientationClockwise
Centisome Percentage:24.78
Left Sequence End1149893
Right Sequence End1150627
Gene Sequence:
>735 bp
ATGAAAATTGACCTGAACGCCGATCTGGGCGAAGGCTGCGCCAGCGACGCAGAGCTATTA
ACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATG
CAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTT
CCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCC
CAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATG
CGTCATGTCAAACCGCACGGCATGTTGTACAACCAGGCGGCGAAAGAAGCACAACTGGCA
GACGCCATCGCCAGAGCGGTATACGCTTGCGATCCAGCATTGATTCTCGTCGGGCTGGCG
GGAAGCGAGCTGATTCGTGCAGGCAAGCAATATGGTCTGACAACGCGCGAGGAAGTGTTT
GCCGATCGCGGTTATCAGGCTGACGGCTCGCTGGTGCCGCGAAGCCAGTCAGGCGCGTTG
ATTGAAAACGAAGAACAGGCGCTGGCGCAAACGCTGGAGATGGTGCAACACGGCAGAGTC
AAAAGTATCACCGGCGAATGGGCAACGGTCGCGGCGCAAACGGTCTGCCTGCATGGCGAC
GGCGAGCACGCACTGGCGTTCGCCCGCCGACTACGCTCTGCATTTGCCGAAAAGGGGATT
GTTGTCGCAGCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:244
Protein Molecular Weight:25560
Protein Theoretical pI:8
PDB File:1Q7B
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>3-oxoacyl-[acyl-carrier-protein] reductase
MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQAISDYLGANGKGLMLNV
TDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEEWNDIIETNLSSVFRLSKA
VMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIGFSKSLAREVASRGITVNVVA
PGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANAVAFLASDEAAYITGETLHVNGG
MYMV
References
External Links:
ResourceLink
Uniprot ID:P0AEK2
Uniprot Name:FABG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062309
PDB ID:1Q7B
Ecogene ID:EG11318
Ecocyc:EG11318
ColiBase:b1093
Kegg Gene:b1093
EchoBASE ID:EB1294
CCDB:FABG_ECOLI
BacMap:16129056
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Price, A. C., Zhang, Y. M., Rock, C. O., White, S. W. (2001). "Structure of beta-ketoacyl-[acyl carrier protein] reductase from Escherichia coli: negative cooperativity and its structural basis." Biochemistry 40:12772-12781. Pubmed: 11669613
  • Rawlings, M., Cronan, J. E. Jr (1992). "The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes." J Biol Chem 267:5751-5754. Pubmed: 1556094
  • Verwoert, I. I., Verbree, E. C., van der Linden, K. H., Nijkamp, H. J., Stuitje, A. R. (1992). "Cloning, nucleotide sequence, and expression of the Escherichia coli fabD gene, encoding malonyl coenzyme A-acyl carrier protein transacylase." J Bacteriol 174:2851-2857. Pubmed: 1314802