D-galactose-binding periplasmic protein (P0AEE5)

Identification
Name:D-galactose-binding periplasmic protein
Synonyms:
  • GBP
  • D-galactose/ D-glucose-binding protein
  • GGBP
Gene Name:mglB
Enzyme Class:Not Available
Biological Properties
General Function:Carbohydrate transport and metabolism
Specific Function:This protein is involved in the active transport of galactose and glucose. It plays a role in the chemotaxis towards the two sugars by interacting with the trg chemoreceptor
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0D-Galactose → 1.0ADP + 1.0D-Galactose + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0D-Glucose → 1.0ADP + 1.0D-Glucose + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00143D-GalactoseMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b2150
Gene OrientationCounterclockwise
Centisome Percentage:48.22
Left Sequence End2237372
Right Sequence End2238370
Gene Sequence:
>999 bp
ATGAATAAGAAGGTGTTAACCCTGTCTGCTGTGATGGCCAGCATGTTATTCGGTGCCGCT
GCACACGCTGCTGATACTCGCATTGGTGTAACAATCTATAAGTACGACGATAACTTTATG
TCTGTAGTGCGCAAGGCTATTGAGCAAGATGCGAAAGCCGCGCCAGATGTTCAGCTGCTG
ATGAATGATTCTCAGAATGACCAGTCCAAGCAGAACGATCAGATCGACGTATTGCTGGCG
AAAGGGGTGAAGGCACTGGCAATCAACCTGGTTGACCCGGCAGCTGCGGGTACGGTGATT
GAGAAAGCGCGTGGGCAAAACGTGCCGGTGGTTTTCTTCAACAAAGAACCGTCTCGTAAG
GCGCTGGATAGCTACGACAAAGCCTACTACGTTGGCACTGACTCCAAAGAGTCCGGCATT
ATTCAAGGCGATTTGATTGCTAAACACTGGGCGGCGAATCAGGGTTGGGATCTGAACAAA
GACGGTCAGATTCAGTTCGTACTGCTGAAAGGTGAACCGGGCCATCCGGATGCAGAAGCA
CGTACCACTTACGTGATTAAAGAATTGAACGATAAAGGCATCAAAACTGAACAGTTACAG
TTAGATACCGCAATGTGGGACACCGCTCAGGCGAAAGATAAGATGGACGCCTGGCTGTCT
GGCCCGAACGCCAACAAAATCGAAGTGGTTATCGCCAACAACGATGCGATGGCAATGGGC
GCGGTTGAAGCGCTGAAAGCACACAACAAGTCCAGCATTCCGGTGTTTGGCGTCGATGCG
CTGCCAGAAGCGCTGGCGCTGGTGAAATCCGGTGCACTGGCGGGCACCGTACTGAACGAT
GCTAACAACCAGGCGAAAGCGACCTTTGATCTGGCGAAAAACCTGGCCGATGGTAAAGGT
GCGGCTGATGGCACCAACTGGAAAATCGACAACAAAGTGGTCCGCGTACCTTATGTTGGC
GTAGATAAAGACAACCTGGCTGAATTCAGCAAGAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:332
Protein Molecular Weight:35712
Protein Theoretical pI:6
PDB File:2GBP
Signaling Regions:
  • 1-23
Transmembrane Regions:
  • None
Protein Sequence:
>D-galactose-binding periplasmic protein
MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVRKAIEQDAKAAPDVQLL
MNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPSRK
ALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNKDGQIQFVLLKGEPGHPDAEA
RTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAWLSGPNANKIEVVIANNDAMAMG
AVEALKAHNKSSIPVFGVDALPEALALVKSGALAGTVLNDANNQAKATFDLAKNLADGKG
AADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK
References
External Links:
ResourceLink
Uniprot ID:P0AEE5
Uniprot Name:DGAL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675264
PDB ID:2GBP
Ecogene ID:EG10593
Ecocyc:EG10593
ColiBase:b2150
Kegg Gene:b2150
EchoBASE ID:EB0588
CCDB:DGAL_ECOLI
BacMap:16130088
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hogg, R. W., Voelker, C., Von Carlowitz, I. (1991). "Nucleotide sequence and analysis of the mgl operon of Escherichia coli K12." Mol Gen Genet 229:453-459. Pubmed: 1719366
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Mahoney, W. C., Hogg, R. W., Hermodson, M. A. (1981). "The amino acid sequence of the D-galactose-binding protein from Escherichia coli B/r." J Biol Chem 256:4350-4356. Pubmed: 7012152
  • Scholle, A., Vreemann, J., Blank, V., Nold, A., Boos, W., Manson, M. D. (1987). "Sequence of the mglB gene from Escherichia coli K12: comparison of wild-type and mutant galactose chemoreceptors." Mol Gen Genet 208:247-253. Pubmed: 3302609
  • Scripture, J. B., Hogg, R. W. (1983). "The nucleotide sequences defining the signal peptides of the galactose-binding protein and the arabinose-binding protein." J Biol Chem 258:10853-10855. Pubmed: 6885805
  • Vyas, N. K., Vyas, M. N., Quiocho, F. A. (1983). "The 3 A resolution structure of a D-galactose-binding protein for transport and chemotaxis in Escherichia coli." Proc Natl Acad Sci U S A 80:1792-1796. Pubmed: 6340108
  • Vyas, N. K., Vyas, M. N., Quiocho, F. A. (1987). "A novel calcium binding site in the galactose-binding protein of bacterial transport and chemotaxis." Nature 327:635-638. Pubmed: 3600760
  • Vyas, N. K., Vyas, M. N., Quiocho, F. A. (1988). "Sugar and signal-transducer binding sites of the Escherichia coli galactose chemoreceptor protein." Science 242:1290-1295. Pubmed: 3057628