Ribosomal RNA small subunit methyltransferase D (P0ADX9)

Identification
Name:Ribosomal RNA small subunit methyltransferase D
Synonyms:
  • 16S rRNA m2G966 methyltransferase
  • rRNA (guanine-N(2)-)-methyltransferase
Gene Name:rsmD
Enzyme Class:
Biological Properties
General Function:Involved in nucleic acid binding
Specific Function:Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0rRNA ↔ 1.0Thumb+1.0rRNA containing N2-methylguanine
1.0S-Adenosylmethionine + 1.0rRNA ↔ 1.0S-Adenosylhomocysteine + 1.0rRNA containing N2-methylguanine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00939S-AdenosylhomocysteineMetaboCard
ECMDB01185S-AdenosylmethionineMetaboCard
GO Classification:
Function
binding
catalytic activity
methyltransferase activity
nucleic acid binding
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular macromolecule metabolic process
cellular metabolic process
macromolecule metabolic process
metabolic process
methylation
ncRNA metabolic process
one-carbon metabolic process
RNA metabolic process
rRNA metabolic process
rRNA methylation
rRNA modification
rRNA processing
Gene Properties
Blattner:b3465
Gene OrientationClockwise
Centisome Percentage:77.64
Left Sequence End3602416
Right Sequence End3603012
Gene Sequence:
>597 bp
ATGATCGAATTTGTTTATCCGCACACGCAGCTGGTTGCGGGTGTGGATGAAGTCGGACGC
GGGCCGTTAGTTGGCGCGGTCGTCACCGCTGCGGTGATCCTTGACCCGGCGCGCCCGATT
GCCGGGCTGAATGATTCCAAAAAGCTGAGCGAAAAACGCCGTCTGGCGCTCTATGAAGAG
ATCAAAGAGAAAGCGTTGAGCTGGAGTCTGGGCCGCGCGGAACCCCACGAAATCGACGAG
CTGAACATTCTTCATGCGACCATGCTGGCGATGCAGCGTGCCGTCGCTGGGCTGCATATT
GCGCCGGAATATGTGTTGATTGATGGTAACCGCTGCCCGAAATTACCGATGCCTGCGATG
GCTGTGGTGAAAGGCGATAGCCGCGTACCGGAAATCAGTGCCGCGTCTATCCTGGCGAAA
GTGACGCGTGACGCCGAAATGGCGGCGCTGGATATTGTTTTCCCGCAATATGGTTTTGCC
CAACACAAAGGGTACCCAACCGCTTTTCATCTGGAAAAACTGGCTGAACACGGCGCGACC
GAACACCATCGGCGCAGCTTTGGGCCTGTCAAACGCGCACTGGGACTTGCGTCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:198
Protein Molecular Weight:21677
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ribosomal RNA small subunit methyltransferase D
MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIVDAQCLDCF
AGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGNARVVNSNAMSFLAQKGTP
HNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEVENGLPTVPANWSLHREKVAG
QVAYRLYQREAQGESDAD
References
External Links:
ResourceLink
Uniprot ID:P0ADX9
Uniprot Name:RSMD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674372
Ecogene ID:EG10343
Ecocyc:EG10343
ColiBase:b3465
Kegg Gene:b3465
EchoBASE ID:EB0339
CCDB:RSMD_ECOLI
BacMap:16131337
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gill, D. R., Hatfull, G. F., Salmond, G. P. (1986). "A new cell division operon in Escherichia coli." Mol Gen Genet 205:134-145. Pubmed: 3025556
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lesnyak, D. V., Osipiuk, J., Skarina, T., Sergiev, P. V., Bogdanov, A. A., Edwards, A., Savchenko, A., Joachimiak, A., Dontsova, O. A. (2007). "Methyltransferase that modifies guanine 966 of the 16 S rRNA: functional identification and tertiary structure." J Biol Chem 282:5880-5887. Pubmed: 17189261
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620