ECMDB: Aspartate ammonia-lyase (P0AC38)

Identification

Name:

Aspartate ammonia-lyase

Synonyms:

Aspartase

Gene Name:

aspA

Enzyme Class:

4.3.1.1

Biological Properties

General Function:

Involved in aspartate ammonia-lyase activity

Specific Function:

L-aspartate = fumarate + NH(3)

Cellular Location:

Not Available

SMPDB Pathways:

Aspartate metabolism PW000787
L-glutamate metabolism PW000789
Nitrogen metabolism PW000755

KEGG Pathways:

Alanine, aspartate and glutamate metabolism ec00250
Metabolic pathways eco01100
Nitrogen metabolism ec00910

KEGG Reactions:

1.0

↔

1.0

1.0L-Aspartic acid ↔ 1.0Fumaric acid + 1.0Ammonia
ReactionCard

1.0

↔

1.0

1.0L-Aspartic acid ↔ 1.0Hydrogen ion + 1.0Fumaric acid + 1.0Ammonia
ReactionCard

SMPDB Reactions:

1.0L-Aspartic acid

1.0

→

1.0

1.0L-Aspartic acid + 1.0L-Aspartic acid → 1.0Fumaric acid + 1.0Ammonia
ReactionCard

1.0L-Aspartic acid

1.0

→

1.0

1.0L-Aspartic acid + 1.0L-Aspartic acid → 1.0Fumaric acid + 1.0Ammonium
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0L-Aspartic acid ↔ 1.0Hydrogen ion + 1.0Fumaric acid + 1.0Ammonia
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0L-Aspartic acid → 1.0Fumaric acid + 1.0Ammonium
ReactionCard

1.0

→

1.0

1.0L-Aspartic acid → 1.0Fumaric acid + 1.0Ammonia
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00051	Ammonia	MetaboCard
ECMDB21186	Ammonium	MetaboCard
ECMDB00176	Fumaric acid	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00191	L-Aspartic acid	MetaboCard

GO Classification:

Function
ammonia-lyase activity
aspartate ammonia-lyase activity
carbon-nitrogen lyase activity
catalytic activity
lyase activity
Process
acetyl-CoA catabolic process
acetyl-CoA metabolic process
aspartate family amino acid metabolic process
aspartate metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
tricarboxylic acid cycle

Gene Properties

Blattner:

b4139

Gene Orientation

Counterclockwise

Centisome Percentage:

94.08

Left Sequence End

4364914

Right Sequence End

4366350

Gene Sequence:

>1437 bp
ATGACAAGGATAAAAATAAACGCACGCCGTATCTTCAGCTTATTGATTCCTTTTTTCTTT
TTCACTTCTGTTCACGCTGAACAAACGGCTGCTCCCGCAAAACCTGTAACTGTGGAAGCG
AAGAATGAAACCTTTGCCCCGCAGCATCCCGATCAATATCTCTCCTGGAAAGCCACCTCG
GAACAGTCAGAGCGTGTTGACGCCCTGGCGGAAGATCCACGGCTGGTGATCCTGTGGGCG
GGGTATCCCTTCTCGCGCGATTACAACAAGCCGCGTGGACATGCTTTTGCTGTGACCGAT
GTGCGTGAAACCCTGCGTACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATG
GCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGAT
GGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGTCAACAACTTAGGTTGT
GCCGATTGCCATAACACCGCCTCTCCAGAGTTCGCCAAAGGCAAACCGGAGTTAACCCTT
TCCCGTCCGTATGCGGCTCGCGCGATGGAAGCCATTGGTAAACCTTTTGAGAAAGCCGGA
CGTTTCGACCAGCAATCGATGGTTTGCGGTCAGTGCCATGTGGAGTATTACTTCGACGGC
AAAAACAAAGCGGTTAAATTCCCGTGGGATGACGGCATGAAAGTCGAAAATATGGAGCAG
TATTACGACAAAATTGCCTTCTCTGACTGGACTAACTCCCTGTCGAAAACGCCAATGCTG
AAAGCGCAGCACCCGGAATATGAAACCTGGACAGCGGGCATTCACGGTAAAAACAACGTG
ACCTGTATCGACTGCCATATGCCAAAAGTGCAGAACGCCGAAGGCAAACTCTACACCGAC
CATAAAATTGGTAATCCGTTTGATAACTTCGCCCAGACTTGTGCGAACTGCCATACCCAG
GACAAAGCTGCCTTGCAAAAAGTGGTCGCGGAACGTAAGCAGTCGATTAACGACCTGAAA
ATCAAGGTTGAAGATCAACTGGTTCACGCTCACTTCGAAGCGAAAGCAGCGCTGGATGCA
GGCGCGACGGAAGCTGAAATGAAGCCAATTCAGGACGATATCCGTCATGCCCAGTGGCGC
TGGGATCTGGCGATCGCTTCCCACGGCATTCATATGCACGCACCGGAAGAAGGTTTACGG
ATGCTCGGTACGGCGATGGATAAAGCGGCGGATGCACGCACCAAACTGGCGCGCCTGCTG
GCGACCAAAGGCATCACCCATGAAATCCAGATCCCGGATATCTCAACCAAAGAGAAAGCC
CAGCAGGCCATTGGCCTGAACATGGAACAAATCAAGGCCGAGAAGCAGGACTTCATCAAA
ACGGTGATCCCGCAGTGGGAAGAACAGGCACGTAAAAACGGTCTGTTAAGCCAATAA

Protein Properties

Pfam Domain Function:

FumaraseC_C (PF10415 )
Lyase_1 (PF00206 )

Protein Residues:

478

Protein Molecular Weight:

52356

Protein Theoretical pI:

PDB File:

1JSW

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Aspartate ammonia-lyase
MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDIPEFVRGMVMVKKAAAM
ANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGAGTSVNMNTNEVLANIGLE
LMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSLIKLVDAINQLREGFERKAVE
FQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNIQRTAELLLEVNLGATAIGTGLN
TPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGAYVMVHGALKRLAVKMSKICNDLRL
LSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEVVNQVCFKVIGNDTTVTMAAEAGQLQ
LNVMEPVIGQAMFESVHILTNACYNLLEKCINGITANKEVCEGYVYNSIGIVTYLNPFIG
HHNGDIVGKICAETGKSVREVVLERGLLTEAELDDIFSVQNLMHPAYKAKRYTDESEQ

References

External Links:

Resource	Link
Uniprot ID:	P0AC38
Uniprot Name:	ASPA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85676822
PDB ID:	1JSW
Ecogene ID:	EG10095
Ecocyc:	EG10095
ColiBase:	b4139
Kegg Gene:	b4139
EchoBASE ID:	EB0093
CCDB:	ASPA_ECOLI
BacMap:	90111690

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Jayasekera, M. M., Shi, W., Farber, G. K., Viola, R. E. (1997). "Evaluation of functionally important amino acids in L-aspartate ammonia-lyase from Escherichia coli." Biochemistry 36:9145-9150. Pubmed: 9230046
Saribas, A. S., Schindler, J. F., Viola, R. E. (1994). "Mutagenic investigation of conserved functional amino acids in Escherichia coli L-aspartase." J Biol Chem 269:6313-6319. Pubmed: 8119980
Shi, W., Dunbar, J., Jayasekera, M. M., Viola, R. E., Farber, G. K. (1997). "The structure of L-aspartate ammonia-lyase from Escherichia coli." Biochemistry 36:9136-9144. Pubmed: 9230045
Takagi, J. S., Ida, N., Tokushige, M., Sakamoto, H., Shimura, Y. (1985). "Cloning and nucleotide sequence of the aspartase gene of Escherichia coli W." Nucleic Acids Res 13:2063-2074. Pubmed: 2987841
Woods, S. A., Miles, J. S., Roberts, R. E., Guest, J. R. (1986). "Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12." Biochem J 237:547-557. Pubmed: 3541901