2-hydroxy-3-oxopropionate reductase (P0ABQ2)

Identification
Name:2-hydroxy-3-oxopropionate reductase
Synonyms:
  • Tartronate semialdehyde reductase
  • TSAR
Gene Name:garR
Enzyme Class:
Biological Properties
General Function:Involved in 3-hydroxyisobutyrate dehydrogenase activity
Specific Function:(R)-glycerate + NAD(P)(+) = 2-hydroxy-3- oxopropanoate + NAD(P)H
Cellular Location:Not Available
SMPDB Pathways:
  • superpathway of D-glucarate and D-galactarate degradation PW000795
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Tartronate semialdehyde + 1.0Hydrogen ion + 1.0NADH ↔ 1.0Glyceric acid + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NAD ↔ 1.0Tartronate semialdehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NADP ↔ 1.0Tartronate semialdehyde + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NAD + 1.0NADP ↔ 1.0Tartronate semialdehyde + 1.0NADH + 1.0NADPH + 1.0Hydrogen ion
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.05-dehydro-4-deoxy-D-glucarate(2−) → 1.0Pyruvic acid + 1.0Tartronate semialdehyde
ReactionCard
1.0Thumb+1.0Thumb+1.0NADPH+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Tartronate semialdehyde + 1.0Hydrogen ion + 1.0NADPH + 1.0NADPH → 1.0NADP + 1.0Glyceric acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Tartronate semialdehyde + 1.0Hydrogen ion + 1.0NADH ↔ 1.0Glyceric acid + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Glyceric acid + 1.0NAD ↔ 1.0Tartronate semialdehyde + 1.0NADH + 1.0Hydrogen ion
ReactionCard
1.0NAD(P)++1.0Thumb ← 1.0NAD(P)H+1.0Thumb+1.0Thumb
1.0NAD(P)+ + 1.0Glyceric acid ← 1.0NAD(P)H + 1.0Tartronate semialdehyde + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0NAD(P)(+) → 1.0Thumb+1.0NAD(P)H
1.0Glyceric acid + 1.0NAD(P)(+) → 1.0Tartronate semialdehyde + 1.0NAD(P)H
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB000233-Hydroxyisobutyric acidMetaboCard
ECMDB241965-dehydro-4-deoxy-D-glucarate(2−)MetaboCard
ECMDB04077Glyceric acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB01487NADHMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB06938Tartronate semialdehydeMetaboCard
GO Classification:
Function
2-hydroxy-3-oxopropionate reductase activity
3-hydroxyisobutyrate dehydrogenase activity
binding
catalytic activity
coenzyme binding
cofactor binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
phosphogluconate dehydrogenase (decarboxylating) activity
Process
alcohol metabolic process
branched chain family amino acid metabolic process
cellular aldehyde metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glucose catabolic process
glucose metabolic process
glyoxylate metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
oxidation reduction
pentose-phosphate shunt
small molecule metabolic process
valine metabolic process
Gene Properties
Blattner:b3125
Gene OrientationCounterclockwise
Centisome Percentage:70.48
Left Sequence End3269889
Right Sequence End3270773
Gene Sequence:
>885 bp
ATGGCTATGGAAACGCAGGACATTATCAAAAGGTCCGCAACTAACTCCATCACGCCGCCT
TCTCAGGTGCGTGATTACAAAGCAGAAGTCGCAAAACTTATCGACGTTTCCACCTGTATC
GGCTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGG
CACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATG
CGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTGATCCGTAAAGACGGCTGT
ATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAG
TACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATT
GCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGC
ACGCTCTGCGTCGATCGCGTCAGCGTCGGCCAGGAACCGGCTTGTGTGAAAACCTGTCCG
ACCGGGGCTATCCACTTCGGCACCAAGAAGGAGATGCTGGAGCTGGCGGAACAGCGCGTG
GCGAAACTGAAAGCGCGTGGTTACGAACATGCTGGCGTCTACAACCCGGAAGGGGTCGGT
GGTACGCACGTTATGTACGTGCTGCATCACGCCGATCAGCCGGAGCTGTATCACGGTCTG
CCGAAAGATCCGAAGATCGACACCTCGGTAAGCCTGTGGAAAGGCGCGTTGAAACCGCTG
GCAGCGGCTGGCTTTATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGC
CCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:294
Protein Molecular Weight:30427
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-hydroxy-3-oxopropionate reductase
MKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAAGAETASTAKAIAEQCDVI
ITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLASREISEALKAKGIDMLDAPV
SGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVVHTGEIGAGNVTKLANQVIVALN
IAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLDAKAPMVMDRNFKPGFRIDLHIKDL
ANALDTSHGVGAQLPLTAAVMEMMQALRADGLGTADHSALACYYEKLAKVEVTR
References
External Links:
ResourceLink
Uniprot ID:P0ABQ2
Uniprot Name:GARR_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742408
Ecogene ID:EG11176
Ecocyc:EG11176
ColiBase:b3125
Kegg Gene:b3125
EchoBASE ID:EB1163
CCDB:GARR_ECOLI
BacMap:145698315
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hubbard, B. K., Koch, M., Palmer, D. R., Babbitt, P. C., Gerlt, J. A. (1998). "Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli." Biochemistry 37:14369-14375. Pubmed: 9772162
  • Komine, Y., Inokuchi, H. (1991). "Precise mapping of the rnpB gene encoding the RNA component of RNase P in Escherichia coli K-12." J Bacteriol 173:1813-1816. Pubmed: 1705543
  • Monterrubio, R., Baldoma, L., Obradors, N., Aguilar, J., Badia, J. (2000). "A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli." J Bacteriol 182:2672-2674. Pubmed: 10762278