Cytochrome o ubiquinol oxidase subunit 3 (P0ABJ3)

Identification
Name:Cytochrome o ubiquinol oxidase subunit 3
Synonyms:
  • Cytochrome o ubiquinol oxidase subunit III
  • Ubiquinol oxidase chain C
Gene Name:cyoC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in heme-copper terminal oxidase activity
Specific Function:Cytochrome o terminal oxidase complex is the component of the aerobic respiratory chain of E.coli that predominates when cells are grown at high aeration
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
SMPDB Reactions:
1.0Thumb+8.0Thumb+2.0Thumb → 2.0Thumb+8.0Thumb+2.0Thumb
1.0Oxygen + 8.0Hydrogen ion + 2.0Ubiquinol-1 → 2.0Water + 8.0Hydrogen ion + 2.0Ubiquinone-1
ReactionCard
Complex Reactions:
2.0Thumb+0.5Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+2.0Thumb
2.0Hydrogen ion + 0.5Oxygen + 1.0Ubiquinol-8 → 1.0Water + 1.0Ubiquinone-8 + 2.0Hydrogen ion
ReactionCard
4.0Thumb+0.5Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+4.0Thumb
4.0Hydrogen ion + 0.5Oxygen + 1.0Ubiquinol-8 → 1.0Water + 1.0Ubiquinone-8 + 4.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB21574Ubiquinol-1MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21438Ubiquinone-1MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
plasma membrane
Function
catalytic activity
cytochrome-c oxidase activity
heme-copper terminal oxidase activity
oxidoreductase activity
oxidoreductase activity, acting on diphenols and related substances as donors
oxidoreductase activity, acting on diphenols and related substances as donors, oxygen as acceptor
Process
cellular metabolic process
electron transport chain
generation of precursor metabolites and energy
metabolic process
mitochondrial electron transport, cytochrome c to oxygen
oxidation reduction
respiratory electron transport chain
Gene Properties
Blattner:b0430
Gene OrientationCounterclockwise
Centisome Percentage:9.64
Left Sequence End447270
Right Sequence End447884
Gene Sequence:
>615 bp
ATGAATATATTTGAACAAACTCCACCGAACCGCAGACGTTATGGTCTTGCTGCATTCATT
GGGCTGATTGCTGGCGTTGTTTCCGCATTCGTGAAGTGGGGGGCTGAAGTTCCATTGCCG
CCACGTAGCCCGGTGGATATGTTTAATGCAGCGTGTGGCCCGGAATCATTAATCAGGGCT
GCAGGCCAAATTGATTGCTCGCGTAATTTTCTCAATCCACCGTATATTTTTCTTCGAGAC
TGGTTGGGGCTGACAGATCCCAATGCGGCTGTTTATACCTTTGCCGGGCATGTCTTTAAC
TGGGTTGGTGTTACGCACATTATCTTTTCGATAGTGTTTGCTGTCGGTTATTGTGTGGTC
GCTGAAGTATTTCCAAAAATTAAACTCTGGCAGGGCTTACTGGCAGGTGCTTTAGCCCAA
CTTTTTGTTCATATGATTTCATTCCCTCTCATGGGACTGACGCCACCTCTGTTTGATCTC
CCGTGGTATGAGAATGTTTCTGAAATTTTTGGACATTTAGTCTGGTTCTGGTCTATTGAA
ATTATTCGCAGAGATTTACGAAACAGAATTACTCATGAGCCAGACCCTGAGATCCCTTTA
GGCTCAAACAGATAA
Protein Properties
Pfam Domain Function:
Protein Residues:204
Protein Molecular Weight:22623
Protein Theoretical pI:7
PDB File:1FFT
Signaling Regions:
  • None
Transmembrane Regions:
  • 32-50
  • 67-85
  • 102-120
  • 143-161
  • 185-203
Protein Sequence:
>Cytochrome o ubiquinol oxidase subunit 3
MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILFATYAVLVNGTAGGPTG
KDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWLALTWLFGAGFIGMEIYEF
HHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVLMVQIARRGLTSTNRTRIMCL
SLFWHFLDVVWICVFTVVYLMGAM
References
External Links:
ResourceLink
Uniprot ID:P0ABJ3
Uniprot Name:CYOC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674431
PDB ID:1FFT
Ecogene ID:EG10180
Ecocyc:EG10180
ColiBase:b0430
Kegg Gene:b0430
EchoBASE ID:EB0177
CCDB:CYOC_ECOLI
BacMap:16128415
General Reference:
  • Abramson, J., Riistama, S., Larsson, G., Jasaitis, A., Svensson-Ek, M., Laakkonen, L., Puustinen, A., Iwata, S., Wikstrom, M. (2000). "The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site." Nat Struct Biol 7:910-917. Pubmed: 11017202
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Chepuri, V., Gennis, R. B. (1990). "The use of gene fusions to determine the topology of all of the subunits of the cytochrome o terminal oxidase complex of Escherichia coli." J Biol Chem 265:12978-12986. Pubmed: 2165491
  • Chepuri, V., Lemieux, L., Au, D. C., Gennis, R. B. (1990). "The sequence of the cyo operon indicates substantial structural similarities between the cytochrome o ubiquinol oxidase of Escherichia coli and the aa3-type family of cytochrome c oxidases." J Biol Chem 265:11185-11192. Pubmed: 2162835
  • Chepuri, V., Lemieux, L., Hill, J., Alben, J. O., Gennis, R. B. (1990). "Recent studies of the cytochrome o terminal oxidase complex of Escherichia coli." Biochim Biophys Acta 1018:124-127. Pubmed: 2168206
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553