Lipopolysaccharide export system ATP-binding protein lptB (P0A9V1)

Identification
Name:Lipopolysaccharide export system ATP-binding protein lptB
Synonyms:Not Available
Gene Name:lptB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex lptBFG involved in the translocation of lipopolysaccharide (LPS) from the inner membrane to the outer membrane. Probably responsible for energy coupling to the transport system
Cellular Location:Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:Not Available
Complex Reactions:
1.0Thumb+1.0Thumb+1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A → 1.0Thumb+1.0Thumb+1.0Thumb+1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0Water + 1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0(O16 antigen)x4 core oligosaccharide lipid A → 1.0Thumb+1.0Thumb+1.0Thumb+1.0(O16 antigen)x4 core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0Water + 1.0(O16 antigen)x4 core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0(O16 antigen)x4 core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A) → 1.0Thumb+1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A)
1.0Adenosine triphosphate + 1.0Water + 1.0cold adapted KDO(2)-lipid (A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0cold adapted KDO(2)-lipid (A)
ReactionCard
1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A → 1.0Thumb+1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0Water + 1.0core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A diphosphate → 1.0Thumb+1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A diphosphate
1.0Adenosine triphosphate + 1.0Water + 1.0core oligosaccharide lipid A diphosphate → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0core oligosaccharide lipid A diphosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO2-Lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO2-Lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.04-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.04-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO(2)-lipid IV(A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO(2)-lipid IV(A)
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Phosphoethanolamine KDO(2)-lipid (A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0Phosphoethanolamine KDO(2)-lipid (A)
ReactionCard
1.0Thumb+1.0core oligosaccharide lipid A+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0core oligosaccharide lipid A
1.0Adenosine triphosphate + 1.0core oligosaccharide lipid A + 1.0Water → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0core oligosaccharide lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A) → 1.0Thumb+1.0Thumb+1.0Thumb+1.0cold adapted KDO(2)-lipid (A)
1.0Adenosine triphosphate + 1.0Water + 1.0cold adapted KDO(2)-lipid (A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0cold adapted KDO(2)-lipid (A)
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO2-Lipid A → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO2-Lipid A
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0KDO(2)-lipid IV(A) → 1.0ADP + 1.0Hydrogen ion + 1.0Phosphate + 1.0KDO(2)-lipid IV(A)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211734-Amino-4-deoxy-L-arabinose modified core oligosaccharide lipid AMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21235KDO(2)-lipid IV(A)MetaboCard
ECMDB21031KDO2-Lipid AMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB21352Phosphoethanolamine KDO(2)-lipid (A)MetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATPase activity
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity
Gene Properties
Blattner:b3201
Gene OrientationClockwise
Centisome Percentage:72.03
Left Sequence End3341966
Right Sequence End3342691
Gene Sequence:
>726 bp
ATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACC
CGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATC
ATCAACCTTGAGAAAACTGTACCGATGTTCAACGAAGCTCTGGCTGAACTGAACAAGATT
GCTTCTCGCAAAGGTAAAATCCTTTTCGTTGGTACTAAACGCGCTGCAAGCGAAGCGGTG
AAAGACGCTGCTCTGAGCTGCGACCAGTTCTTCGTGAACCATCGCTGGCTGGGCGGTATG
CTGACTAACTGGAAAACCGTTCGTCAGTCCATCAAACGTCTGAAAGACCTGGAAACTCAG
TCTCAGGACGGTACTTTCGACAAGCTGACCAAGAAAGAAGCGCTGATGCGCACTCGTGAG
CTGGAGAAACTGGAAAACAGCCTGGGCGGTATCAAAGACATGGGCGGTCTGCCGGACGCT
CTGTTTGTAATCGATGCTGACCACGAACACATTGCTATCAAAGAAGCAAACAACCTGGGT
ATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATC
CCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCAACC
GTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCT
GAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:241
Protein Molecular Weight:26800
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide export system ATP-binding protein lptB
MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTTTFYMVVGIVPRDAGNI
IIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAVLQIRDDLSAEQREDRANE
LMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFILLDEPFAGVDPISVIDIKRII
EHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAHGTPTEILQDEHVKRVYLGEDFR
L
References
External Links:
ResourceLink
Uniprot ID:P0A9V1
Uniprot Name:LPTB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21239023
Ecogene ID:EG11680
Ecocyc:EG11680
ColiBase:b3201
Kegg Gene:b3201
EchoBASE ID:EB1631
CCDB:LPTB_ECOLI
BacMap:16131091
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Imaishi, H., Gomada, M., Inouye, S., Nakazawa, A. (1993). "Physical map location of the rpoN gene of Escherichia coli." J Bacteriol 175:1550-1551. Pubmed: 8444818
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Powell, B. S., Court, D. L., Inada, T., Nakamura, Y., Michotey, V., Cui, X., Reizer, A., Saier, M. H. Jr, Reizer, J. (1995). "Novel proteins of the phosphotransferase system encoded within the rpoN operon of Escherichia coli. Enzyme IIANtr affects growth on organic nitrogen and the conditional lethality of an erats mutant." J Biol Chem 270:4822-4839. Pubmed: 7876255
  • Sperandeo, P., Cescutti, R., Villa, R., Di Benedetto, C., Candia, D., Deho, G., Polissi, A. (2007). "Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli." J Bacteriol 189:244-253. Pubmed: 17056748
  • Sperandeo, P., Lau, F. K., Carpentieri, A., De Castro, C., Molinaro, A., Deho, G., Silhavy, T. J., Polissi, A. (2008). "Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli." J Bacteriol 190:4460-4469. Pubmed: 18424520
  • Stenberg, F., Chovanec, P., Maslen, S. L., Robinson, C. V., Ilag, L. L., von Heijne, G., Daley, D. O. (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280:34409-34419. Pubmed: 16079137