Xanthine phosphoribosyltransferase (P0A9M5)

Identification
Name:Xanthine phosphoribosyltransferase
Synonyms:
  • Xanthine-guanine phosphoribosyltransferase
  • XGPRT
Gene Name:gpt
Enzyme Class:
Biological Properties
General Function:Involved in nucleoside metabolic process
Specific Function:Acts on guanine, xanthine and to a lesser extent hypoxanthine
Cellular Location:Cell inner membrane; Peripheral membrane protein (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Guanine + 1.0Phosphoribosyl pyrophosphate → 1.0Guanosine monophosphate + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hypoxanthine + 1.0Phosphoribosyl pyrophosphate ↔ 1.0Inosinic acid + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphoribosyl pyrophosphate + 1.0Xanthine ↔ 1.0Pyrophosphate + 1.0Xanthylic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Guanosine monophosphate + 1.0Pyrophosphate ↔ 1.0Guanine + 1.0Phosphoribosyl pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Xanthylic acid + 1.0Pyrophosphate ↔ 1.0Xanthine + 1.0Phosphoribosyl pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ← 1.0Thumb+1.0Thumb
1.0Xanthylic acid + 1.0Pyrophosphate ← 1.0Xanthine + 1.0Phosphoribosyl pyrophosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Pyrophosphate
1.0Hypoxanthine + 1.0Phosphoribosyl pyrophosphate → 1.0Inosinic acid + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Guanine + 1.0Phosphoribosyl pyrophosphate → 1.0Pyrophosphate + 1.0Guanosine monophosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Xanthine + 1.0Phosphoribosyl pyrophosphate → 1.0Xanthylic acid + 1.0Pyrophosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Guanine + 1.0Phosphoribosyl pyrophosphate → 1.0Guanosine monophosphate + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hypoxanthine + 1.0Phosphoribosyl pyrophosphate ↔ 1.0Inosinic acid + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphoribosyl pyrophosphate + 1.0Xanthine ↔ 1.0Pyrophosphate + 1.0Xanthylic acid
ReactionCard
1.0Thumb+1.0Thumb ← 1.0Thumb+1.0Thumb
1.0Xanthylic acid + 1.0Pyrophosphate ← 1.0Xanthine + 1.0Phosphoribosyl pyrophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Xanthylic acid + 1.0Pyrophosphate → 1.0Phosphoribosyl pyrophosphate + 1.0Xanthine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00132GuanineMetaboCard
ECMDB01397Guanosine monophosphateMetaboCard
ECMDB00157HypoxanthineMetaboCard
ECMDB04085Inosinic acidMetaboCard
ECMDB00280Phosphoribosyl pyrophosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB00292XanthineMetaboCard
ECMDB01554Xanthylic acidMetaboCard
GO Classification:
Process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process
Gene Properties
Blattner:b0238
Gene OrientationClockwise
Centisome Percentage:5.52
Left Sequence End255977
Right Sequence End256435
Gene Sequence:
>459 bp
ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACC
CGTTATCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATT
TATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTA
TCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCAT
GCCAGCGAATGTCAGATGGATGGCGCAGGTCGATTGTTAATCGCGCCAGTACTGCGGCAA
CATGCCGGGCTGACAAAAGAAGTGATGCTGGTTGGACAGTTCAACAAGTTTGAGCTGTGG
GATGAAACAACCTGGCATCAACAGGTCAAGGAAGATATCGACGCAGAGCAGTTGGCTACC
GGAGACTTATCGGAGCGACTGCAGGACTTGTCTCTATAA
Protein Properties
Pfam Domain Function:
Protein Residues:152
Protein Molecular Weight:16971
Protein Theoretical pI:6
PDB File:1A96
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Xanthine phosphoribosyltransferase
MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGALLARELGIRHVDTVCI
SSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIREMYPKAHFVTIFAKPAGRP
LVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR
References
External Links:
ResourceLink
Uniprot ID:P0A9M5
Uniprot Name:XGPT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321962
PDB ID:1A96
Ecogene ID:EG10414
Ecocyc:EG10414
ColiBase:b0238
Kegg Gene:b0238
EchoBASE ID:EB0409
CCDB:XGPT_ECOLI
BacMap:16128224
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Deo, S. S., Tseng, W. C., Saini, R., Coles, R. S., Athwal, R. S. (1985). "Purification and characterization of Escherichia coli xanthine-guanine phosphoribosyltransferase produced by plasmid pSV2gpt." Biochim Biophys Acta 839:233-239. Pubmed: 3886014
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jagadeeswaran, P., Ashman, C. R., Roberts, S., Langenberg, J. (1984). "Nucleotide sequence and analysis of deletion mutants of the Escherichia coli gpt gene in plasmid pSV2 gpt." Gene 31:309-313. Pubmed: 6396164
  • Mulligan, R. C., Berg, P. (1981). "Factors governing the expression of a bacterial gene in mammalian cells." Mol Cell Biol 1:449-459. Pubmed: 6100966
  • Nuesch, J., Schumperli, D. (1984). "Structural and functional organization of the gpt gene region of Escherichia coli." Gene 32:243-249. Pubmed: 6397401
  • Pratt, D., Subramani, S. (1983). "Nucleotide sequence of the Escherichia coli xanthine-guanine phosphoribosyl transferase gene." Nucleic Acids Res 11:8817-8823. Pubmed: 6324103
  • Richardson, K. K., Fostel, J., Skopek, T. R. (1983). "Nucleotide sequence of the xanthine guanine phosphoribosyl transferase gene of E. coli." Nucleic Acids Res 11:8809-8816. Pubmed: 6324102
  • Richardson, K. K., Richardson, F. C., Crosby, R. M., Swenberg, J. A., Skopek, T. R. (1987). "DNA base changes and alkylation following in vivo exposure of Escherichia coli to N-methyl-N-nitrosourea or N-ethyl-N-nitrosourea." Proc Natl Acad Sci U S A 84:344-348. Pubmed: 3540961
  • Vos, S., de Jersey, J., Martin, J. L. (1996). "Crystallization and preliminary X-ray crystallographic studies of Escherichia coli xanthine phosphoribosyltransferase." J Struct Biol 116:330-334. Pubmed: 8812991
  • Vos, S., de Jersey, J., Martin, J. L. (1997). "Crystal structure of Escherichia coli xanthine phosphoribosyltransferase." Biochemistry 36:4125-4134. Pubmed: 9100006
  • Vos, S., Parry, R. J., Burns, M. R., de Jersey, J., Martin, J. L. (1998). "Structures of free and complexed forms of Escherichia coli xanthine-guanine phosphoribosyltransferase." J Mol Biol 282:875-889. Pubmed: 9743633