ECMDB

Identification

Name:

Acyl-CoA thioester hydrolase YbgC

Synonyms:

Acyl-CoA thioesterase

Gene Name:

ybgC

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in hydrolase activity

Specific Function:

Thioesterase that appears to be involved in phospholipid metabolism. Some specific acyl-ACPs could be physiological substrates. Displays acyl-CoA thioesterase activity on malonyl-CoA in vitro, catalyzing the hydrolysis of the thioester bond

Cellular Location:

Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

EcoCyc Reactions:

1.0

→

1.0

1.01,4-Dihydroxy-2-naphthoyl-CoA + 1.0Water → 1.0Coenzyme A + 1.01,4-Dihydroxy-2-naphthoic acid + 1.0Hydrogen ion
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB04054	1,4-Dihydroxy-2-naphthoic acid	MetaboCard
ECMDB20032	1,4-Dihydroxy-2-naphthoyl-CoA	MetaboCard
ECMDB01423	Coenzyme A	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
protein binding
thiolester hydrolase activity

Gene Properties

Blattner:

b0736

Gene Orientation

Clockwise

Centisome Percentage:

16.68

Left Sequence End

773975

Right Sequence End

774379

Gene Sequence:

>405 bp
GTGATATGCTCACCTCAGAACAACACAGGTGTCATAATGAAAAAAAGAAATTTCAGCGCA
GAGTTTAAACGCGAATCCGCTCAACTGGTCGTTGACCAGAACTACACCGTGGCAGATGCA
GCCAGCGCTATGGATGTCGGCCTTTCCACAATGACGCGATGGGTGAAACAATTACGTGAT
GAACGGCAGGGCAAAACACCAAAAGCCTCCCCCATTACCCCGGAACAAATTGAAATCCGT
GAGCTCAGGAAAAAGCTACAACGTATTGAAATGGAAAATGAAATATTAAAAAAGGCTACT
GTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTG
AATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGA

Protein Properties

Pfam Domain Function:

4HBT (PF03061 )

Protein Residues:

134

Protein Molecular Weight:

15562

Protein Theoretical pI:

PDB File:

1S5U

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Acyl-CoA thioester hydrolase YbgC
MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHHFSQQALMAERVAFVVR
KMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENTLLNEAEVLVVCVDPLKMK
PRALPKSIVAEFKQ

References

External Links:

Resource	Link
Uniprot ID:	P0A8Z3
Uniprot Name:	YBGC_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4902991
PDB ID:	1S5U
Ecogene ID:	EG11110
Ecocyc:	EG11110
ColiBase:	b0736
Kegg Gene:	b0736
EchoBASE ID:	EB1101
CCDB:	YBGC_ECOLI
BacMap:	16128711

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Fountoulakis, M., Takacs, M. F., Berndt, P., Langen, H., Takacs, B. (1999). "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Electrophoresis 20:2181-2195. Pubmed: 10493123
Gully, D., Bouveret, E. (2006). "A protein network for phospholipid synthesis uncovered by a variant of the tandem affinity purification method in Escherichia coli." Proteomics 6:282-293. Pubmed: 16294310
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Kuznetsova, E., Proudfoot, M., Sanders, S. A., Reinking, J., Savchenko, A., Arrowsmith, C. H., Edwards, A. M., Yakunin, A. F. (2005). "Enzyme genomics: Application of general enzymatic screens to discover new enzymes." FEMS Microbiol Rev 29:263-279. Pubmed: 15808744
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
Sun, T. P., Webster, R. E. (1987). "Nucleotide sequence of a gene cluster involved in entry of E colicins and single-stranded DNA of infecting filamentous bacteriophages into Escherichia coli." J Bacteriol 169:2667-2674. Pubmed: 3294803

Acyl-CoA thioester hydrolase YbgC (P0A8Z3)