Queuine tRNA-ribosyltransferase (P0A847)

Identification
Name:Queuine tRNA-ribosyltransferase
Synonyms:
  • Guanine insertion enzyme
  • tRNA-guanine transglycosylase
Gene Name:tgt
Enzyme Class:
Biological Properties
General Function:Involved in queuine tRNA-ribosyltransferase activity
Specific Function:Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis- dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine)
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0Queuine + 1.07-Aminomethyl-7-carbaguanine ↔ 1.0Guanine
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0tRNA guanine → 1.07-aminomethyl-7-deazaguanosine34 in tRNA+1.0Thumb
1.07-Aminomethyl-7-carbaguanine + 1.0tRNA guanine → 1.07-aminomethyl-7-deazaguanosine34 in tRNA + 1.0Guanine
ReactionCard
Complex Reactions:
1.0Guanine(34) in tRNA+1.0Thumb → 1.0queuosine(34) in tRNA+1.0Thumb
1.0Guanine(34) in tRNA + 1.0Queuine → 1.0queuosine(34) in tRNA + 1.0Guanine
ReactionCard
1.0Guanine(34) in tRNA+1.0Thumb → 1.07-aminomethyl-7-carbaguanine(34) in tRNA+1.0Thumb
1.0Guanine(34) in tRNA + 1.07-Aminomethyl-7-carbaguanine → 1.07-aminomethyl-7-carbaguanine(34) in tRNA + 1.0Guanine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB214567-Aminomethyl-7-carbaguanineMetaboCard
ECMDB00132GuanineMetaboCard
ECMDB01495QueuineMetaboCard
GO Classification:
Function
catalytic activity
queuine tRNA-ribosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
queuosine biosynthetic process
RNA metabolic process
tRNA metabolic process
tRNA modification
tRNA processing
Gene Properties
Blattner:b0406
Gene OrientationClockwise
Centisome Percentage:9.17
Left Sequence End425361
Right Sequence End426488
Gene Sequence:
>1128 bp
ATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGTGGCCGCCTGGTCTTTGAT
CGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGG
ATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCAC
CTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATG
CAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGAT
ATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGATCCGATT
TTCCTCGATCCTGAAAAATCAATGGAGATTCAGTACGATCTTGGTTCGGATATCGTCATG
ATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCCATGGAG
ATGTCTCTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTCGGAAACAAAAAT
GCGCTGTTTGGTATCATCCAGGGCAGCGTTTACGAAGATTTACGTGATATTTCTGTTAAA
GGTCTGGTAGATATCGGTTTTGATGGCTACGCTGTCGGCGGTCTGGCTGTGGGTGAGCCG
AAAGCAGATATGCACCGCATTCTGGAGCATGTATGCCCGCAAATTCCGGCAGACAAACCG
CGTTACCTGATGGGCGTTGGTAAACCAGAAGACCTGGTTGAAGGCGTACGTCGTGGTATC
GATATGTTTGACTGCGTAATGCCAACCCGCAACGCCCGAAATGGTCATTTGTTCGTGACC
GATGGCGTGGTGAAAATCCGCAATGCGAAGTATAAGAGCGATACTGGCCCACTCGATCCT
GAGTGTGATTGCTACACCTGTCGCAATTATTCACGCGCTTACTTGCATCATCTTGACCGT
TGCAACGAAATATTAGGCGCGCGACTCAACACCATTCATAACCTTCGTTACTACCAGCGT
TTGATGGCGGGTTTACGCAAGGCTATTGAAGAGGGTAAATTAGAGAGCTTCGTAACTGAT
TTTTACCAGCGTCAGGGGCGAGAAGTACCACCTTTGAACGTTGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:375
Protein Molecular Weight:42593
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Queuine tRNA-ribosyltransferase
MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH
LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI
FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKN
ALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDP
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTD
FYQRQGREVPPLNVD
References
External Links:
ResourceLink
Uniprot ID:P0A847
Uniprot Name:TGT_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674546
Ecogene ID:EG10996
Ecocyc:EG10996
ColiBase:b0406
Kegg Gene:b0406
EchoBASE ID:EB0989
CCDB:TGT_ECOLI
BacMap:16128391
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Chong, S., Curnow, A. W., Huston, T. J., Garcia, G. A. (1995). "tRNA-guanine transglycosylase from Escherichia coli is a zinc metalloprotein. Site-directed mutagenesis studies to identify the zinc ligands." Biochemistry 34:3694-3701. Pubmed: 7893665
  • Garcia, G. A., Chong, S. (1997). "Cysteine 265 is in the active site of, but is not essential for catalysis by tRNA-guanine transglycosylase (TGT) from Escherichia coli." J Protein Chem 16:11-17. Pubmed: 9055203
  • Garcia, G. A., Koch, K. A., Chong, S. (1993). "tRNA-guanine transglycosylase from Escherichia coli. Overexpression, purification and quaternary structure." J Mol Biol 231:489-497. Pubmed: 8323579
  • Gardel, C., Johnson, K., Jacq, A., Beckwith, J. (1990). "The secD locus of E. coli codes for two membrane proteins required for protein export." EMBO J 9:4205-4206. Pubmed: 2249673
  • Gardel, C., Johnson, K., Jacq, A., Beckwith, J. (1990). "The secD locus of E.coli codes for two membrane proteins required for protein export." EMBO J 9:3209-3216. Pubmed: 2170107
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pogliano, K. J., Beckwith, J. (1994). "Genetic and molecular characterization of the Escherichia coli secD operon and its products." J Bacteriol 176:804-814. Pubmed: 7507921
  • Reuter, K., Chong, S., Ullrich, F., Kersten, H., Garcia, G. A. (1994). "Serine 90 is required for enzymic activity by tRNA-guanine transglycosylase from Escherichia coli." Biochemistry 33:7041-7046. Pubmed: 8003468
  • Reuter, K., Slany, R., Ullrich, F., Kersten, H. (1991). "Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes." J Bacteriol 173:2256-2264. Pubmed: 1706703