L-seryl-tRNA(Sec) selenium transferase (P0A821)

Identification
Name:L-seryl-tRNA(Sec) selenium transferase
Synonyms:
  • Selenocysteine synthase
  • Sec synthase
  • Selenocysteinyl-tRNA(Sec) synthase
Gene Name:selA
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Converts seryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis. Requires selenophosphate as the selenium-donor molecule
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0L-Seryl-tRNA(Sec)+1.0Thumb+1.0L-Seryl-tRNA(Sec) ↔ 1.0L-Selenocysteinyl-tRNA(Sec)+1.0Thumb+1.0L-Selenocysteinyl-tRNA(Sec)
1.0L-Seryl-tRNA(Sec) + 1.0Phosphoroselenoic acid + 1.0L-Seryl-tRNA(Sec) ↔ 1.0L-Selenocysteinyl-tRNA(Sec) + 1.0Phosphate + 1.0L-Selenocysteinyl-tRNA(Sec)
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb+1.0L-Selenocysteinyl-tRNA(Sec)
1.0Phosphoroselenoic acid → 1.0Hydrogen ion + 1.0Phosphate + 1.0L-Selenocysteinyl-tRNA(Sec)
ReactionCard
1.0L-seryl-tRNA(Sec)+1.0Thumb → 1.0L-selenocysteinyl-tRNA(Sec)+1.0Thumb
1.0L-seryl-tRNA(Sec) + 1.0Phosphoroselenoic acid → 1.0L-selenocysteinyl-tRNA(Sec) + 1.0Inorganic phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB03840Phosphoroselenoic acidMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
L-seryl-tRNASec selenium transferase activity
selenotransferase activity
transferase activity
transferase activity, transferring selenium-containing groups
Process
biosynthetic process
cellular macromolecule biosynthetic process
macromolecule biosynthetic process
metabolic process
selenocysteine incorporation
translational elongation
translational readthrough
Gene Properties
Blattner:b3591
Gene OrientationCounterclockwise
Centisome Percentage:80.99
Left Sequence End3757881
Right Sequence End3759272
Gene Sequence:
>1392 bp
ATGAGTCACTCATCCGCCCCCGAAAGGGCTACTGGAGCTGTCATTACAGATTGGCGACCG
GAAGATCCTGCGTTCTGGCAACAACGCGGTCAACGTATTGCCAGCCGCAACCTGTGGATT
TCCGTTCCCTGTCTGCTGCTGGCGTTTTGCGTATGGATGTTGTTCAGCGCTGTTGCGGTG
AACCTACCGAAAGTCGGCTTTAATTTTACGACCGATCAGCTATTTATGTTGACTGCGCTG
CCTTCGGTTTCTGGCGCGTTATTACGTGTTCCATACTCCTTTATGGTTCCTATCTTCGGT
GGTCGTCGCTGGACGGCGTTCAGCACCGGTATTCTGATTATTCCTTGCGTCTGGCTGGGT
TTTGCCGTGCAGGATACCTCCACGCCTTATAGCGTCTTCATCATCATCTCTCTGCTATGC
GGCTTTGCTGGCGCGAACTTCGCATCCAGTATGGCAAACATCAGCTTCTTCTTTCCGAAA
CAGAAGCAGGGTGGCGCGCTGGGTCTGAATGGTGGTCTGGGAAACATGGGCGTCAGCGTC
ATGCAGTTGGTTGCTCCGCTGGTGGTATCACTGTCGATTTTCGCAGTATTTGGTAGCCAG
GGCGTCAAACAGCCGGATGGGACTGAGCTGTATCTGGCGAATGCGTCCTGGATATGGGTG
CCGTTCCTTGCCATCTTCACCATTGCGGCGTGGTTTGGCATGAACGATCTTGCTACCTCG
AAAGCCTCCATCAAGGAGCAGTTGCCGGTACTCAAACGGGGTCATCTGTGGATTATGAGC
CTGCTGTATCTGGCAACCTTCGGCTCCTTCATCGGCTTCTCCGCGGGCTTTGCAATGCTG
TCAAAAACGCAGTTCCCGGATGTTCAGATTCTGCAATACGCTTTCTTCGGGCCGTTTATT
GGTGCGCTGGCGCGTTCTGCAGGTGGTGCATTATCTGACCGTCTGGGCGGAACTCGTGTC
ACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCG
ACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTG
ACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTG
ACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGAAGCGGCA
ACCGACACGGCGGCGGCGCTGGGTTTCATCTCTGCGATTGGCGCGATTGGTGGCTTCTTT
ATCCCGAAAGCGTTTGGTAGCTCGCTGGCATTAACGGGTTCGCCAGTCGGCGCAATGAAG
GTATTTTTGATTTTCTATATCGCCTGCGTAGTGATTACCTGGGCGGTATATGGTCGGCAT
TCTAAAAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:463
Protein Molecular Weight:50607
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>L-seryl-tRNA(Sec) selenium transferase
MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQMLDEAREVIRGSQTLPAW
CENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAEAAVEAVAQAMRSPVTLEY
DLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLMLAATASGKEVVVSRGELVEI
GGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNENTALLMKVHTSNYSIQGFTKAID
EAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEPMPQELIAAGVSLVSFSGDKLLGGP
QAGIIVGKKEMIARLQSHPLKRALRADKMTLAALEATLRLYLHPEALSEKLPTLRLLTRS
AEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLSQIGSGSLPVDRLPSAALTFTPHDGRGSH
LESLAARWRELPVPVIGRIYDGRLWLDLRCLEDEQRFLEMLLK
References
External Links:
ResourceLink
Uniprot ID:P0A821
Uniprot Name:SELA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651617
Ecogene ID:EG10941
Ecocyc:EG10941
ColiBase:b3591
Kegg Gene:b3591
EchoBASE ID:EB0934
CCDB:SELA_ECOLI
BacMap:16131462
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Engelhardt, H., Forchhammer, K., Muller, S., Goldie, K. N., Bock, A. (1992). "Structure of selenocysteine synthase from Escherichia coli and location of tRNA in the seryl-tRNA(sec)-enzyme complex." Mol Microbiol 6:3461-3467. Pubmed: 1474891
  • Forchhammer, K., Bock, A. (1991). "Selenocysteine synthase from Escherichia coli. Analysis of the reaction sequence." J Biol Chem 266:6324-6328. Pubmed: 2007585
  • Forchhammer, K., Leinfelder, W., Boesmiller, K., Veprek, B., Bock, A. (1991). "Selenocysteine synthase from Escherichia coli. Nucleotide sequence of the gene (selA) and purification of the protein." J Biol Chem 266:6318-6323. Pubmed: 2007584
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Tormay, P., Wilting, R., Lottspeich, F., Mehta, P. K., Christen, P., Bock, A. (1998). "Bacterial selenocysteine synthase--structural and functional properties." Eur J Biochem 254:655-661. Pubmed: 9688279