Identification
Name:Dihydroorotate dehydrogenase
Synonyms:
  • DHOdehase
  • DHOD
  • DHODase
  • Dihydroorotate oxidase
Gene Name:pyrD
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:(S)-dihydroorotate + a quinone = orotate + a quinol
Cellular Location:Cell membrane; Peripheral membrane protein
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
1.04,5-Dihydroorotic acid+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.04,5-Dihydroorotic acid + 1.0Ubiquinone-1 + 1.04,5-Dihydroorotic acid → 1.0Ubiquinol-1 + 1.0Orotic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0a ubiquinone1.0Thumb+1.0a ubiquinol
1.04,5-Dihydroorotic acid + 1.0a ubiquinone → 1.0Orotic acid + 1.0a ubiquinol
ReactionCard
1.0Thumb+1.0a menaquinone1.0Thumb+1.0a menaquinol
1.04,5-Dihydroorotic acid + 1.0a menaquinone → 1.0Orotic acid + 1.0a menaquinol
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
1.0Thumb+1.0Menaquinone 81.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB005284,5-Dihydroorotic acidMetaboCard
ECMDB00176Fumaric acidMetaboCard
ECMDB02434HydroquinoneMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB00226Orotic acidMetaboCard
ECMDB23060QuinoneMetaboCard
ECMDB00254Succinic acidMetaboCard
ECMDB21574Ubiquinol-1MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21438Ubiquinone-1MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Component
cell part
membrane
Function
catalytic activity
dihydroorotate dehydrogenase activity
dihydroorotate oxidase activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor
Process
'de novo' pyrimidine base biosynthetic process
cellular aromatic compound metabolic process
cellular metabolic process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
oxidation reduction
pyrimidine base biosynthetic process
pyrimidine base metabolic process
pyrimidine nucleoside monophosphate biosynthetic process
pyrimidine nucleotide biosynthetic process
pyrimidine nucleotide metabolic process
pyrimidine ribonucleoside monophosphate biosynthetic process
UMP biosynthetic process
Gene Properties
Blattner:b0945
Gene OrientationClockwise
Centisome Percentage:21.64
Left Sequence End1003991
Right Sequence End1005001
Gene Sequence:
>1011 bp
ATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAG
TTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAG
AAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTG
GCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGA
TCGATCGAGATCGGTACCGTCACGCCACGTCCACAGCCAGGTAATGACAAGCCGCGTCTC
TTTCGTCTGGTAGATGCCGAAGGTTTGATCAACCGTATGGGCTTTAATAATCTTGGCGTT
GATAACCTCGTAGAGAACGTAAAAAAGGCCCATTATGACGGCGTCCTGGGTATTAACATC
GGCAAAAATAAAGATACGCCAGTGGAGCAGGGCAAAGATGACTATCTGATTTGTATGGAA
AAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGA
TTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAG
CAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCG
GATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGAT
GGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGC
GATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGC
CGCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCG
GTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGT
TTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:336
Protein Molecular Weight:36774
Protein Theoretical pI:8
PDB File:1F76
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Dihydroorotate dehydrogenase
MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPAKPVNCMGLTFKNPLGL
AAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFRLVDAEGLINRMGFNNLGV
DNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICMEKIYAYAGYIAINISSPNTPG
LRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKIAPDLSEEELIQVADSLVRHNID
GVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKSTEIIRRLSLELNGRLPIIGVGGIDS
VIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVTHI
References
External Links:
ResourceLink
Uniprot ID:P0A7E1
Uniprot Name:PYRD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651460
PDB ID:1F76
Ecogene ID:EG10807
Ecocyc:EG10807
ColiBase:b0945
Kegg Gene:b0945
EchoBASE ID:EB0800
CCDB:PYRD_ECOLI
BacMap:16128912
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Larsen, J. N., Jensen, K. F. (1985). "Nucleotide sequence of the pyrD gene of Escherichia coli and characterization of the flavoprotein dihydroorotate dehydrogenase." Eur J Biochem 151:59-65. Pubmed: 2992959
  • Norager, S., Jensen, K. F., Bjornberg, O., Larsen, S. (2002). "E. coli dihydroorotate dehydrogenase reveals structural and functional distinctions between different classes of dihydroorotate dehydrogenases." Structure 10:1211-1223. Pubmed: 12220493
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232