Identification
Name:Manganese transport protein mntH
Synonyms:Not Available
Gene Name:mntH
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:H(+)-stimulated, highly selective, manganese uptake system. Can also transport cadmium, cobalt, iron, zinc and to a lesser extent nickel and copper. Involved in response to reactive oxygen
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB00692IronMetaboCard
ECMDB01333ManganeseMetaboCard
GO Classification:
Component
cell part
membrane
Function
transporter activity
Process
establishment of localization
transport
Gene Properties
Blattner:b2392
Gene OrientationCounterclockwise
Centisome Percentage:54.09
Left Sequence End2509490
Right Sequence End2510728
Gene Sequence:
>1239 bp
ATGACGAACTATCGCGTTGAGAGTAGCAGCGGACGGGCGGCGCGCAAGATGAGGCTCGCA
TTAATGGGACCTGCGTTCATTGCGGCGATTGGTTATATCGATCCCGGTAACTTTGCGACC
AATATTCAGGCGGGTGCTAGCTTCGGCTATCAGCTACTGTGGGTTGTCGTTTGGGCCAAC
CTGATGGCGATGCTGATTCAGATCCTCTCTGCCAAACTAGGGATTGCCACCGGTAAAAAT
CTGGCGGAGCAGATTCGCGATCACTATCCGCGTCCCGTAGTGTGGTTCTATTGGGTTCAG
GCAGAAATTATTGCGATGGCAACCGACCTGGCGGAATTTATTGGTGCGGCGATCGGTTTT
AAACTCATTCTTGGTGTTTCGTTGTTGCAGGGCGCGGTGCTGACGGGGATCGCGACTTTC
CTGATTTTAATGCTGCAACGTCGCGGGCAAAAACCGCTGGAGAAAGTGATTGGCGGGTTA
CTGTTGTTTGTTGCCGCGGCTTACATTGTCGAGTTGATTTTCTCCCAGCCTAACCTGGCG
CAGCTGGGTAAAGGAATGGTGATCCCGAGTTTACCTACTTCGGAAGCGGTCTTCCTGGCA
GCAGGCGTGTTAGGGGCGACGATTATGCCGCATGTGATTTATTTGCACTCCTCGCTCACT
CAGCATTTACATGGCGGTTCGCGTCAACAACGTTATTCCGCCACCAAATGGGATGTGGCT
ATCGCCATGACTATTGCCGGTTTTGTCAATCTGGCGATGATGGCTACAGCTGCGGCGGCG
TTCCACTTTTCCGGTCATACTGGTGTTGCCGATCTTGATGAGGCTTATCTGACGCTGCAA
CCGCTGTTAAGCCACGCTGCGGCAACGGTCTTTGGATTAAGCCTGGTTGCTGCGGGGCTG
TCTTCAACGGTGGTGGGGACACTGGCGGGGCAGGTGGTGATGCAGGGCTTCATTCGCTTT
CATATCCCGCTGTGGGTGCGTCGTACAGTCACCATGTTGCCGTCATTTATTGTCATTCTG
ATGGGATTAGATCCGACACGGATTCTGGTTATGAGTCAGGTACTGTTAAGTTTTGGTATC
GCTCTGGCGCTGGTTCCACTGCTGATTTTCACCAGTGACAGCAAGTTGATGGGCGATCTG
GTGAACAGCAAACGCGTAAAACAGACAGGCTGGGTGATTGTGGTGCTGGTCGTGGCGCTG
AATATCTGGTTGTTGGTGGGGACGGCGCTGGGATTGTAG
Protein Properties
Pfam Domain Function:
Protein Residues:412
Protein Molecular Weight:44193
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 20-39
  • 52-71
  • 96-118
  • 126-145
  • 156-175
  • 197-220
  • 239-258
  • 277-297
  • 328-344
  • 351-370
  • 388-406
Protein Sequence:
>Manganese transport protein mntH
MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQAGASFGYQLLWVVVWAN
LMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAEIIAMATDLAEFIGAAIGF
KLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGLLLFVAAAYIVELIFSQPNLA
QLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSSLTQHLHGGSRQQRYSATKWDVA
IAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAYLTLQPLLSHAAATVFGLSLVAAGL
SSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSFGI
ALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL
References
External Links:
ResourceLink
Uniprot ID:P0A769
Uniprot Name:MNTH_ECOLI
GenBank Gene ID:AF161318
Genebank Protein ID:5670178
Ecogene ID:EG14157
Ecocyc:EG14157
ColiBase:b2392
Kegg Gene:b2392
EchoBASE ID:EB3909
CCDB:MNTH_ECOLI
BacMap:16130324
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Courville, P., Chaloupka, R., Veyrier, F., Cellier, M. F. (2004). "Determination of transmembrane topology of the Escherichia coli natural resistance-associated macrophage protein (Nramp) ortholog." J Biol Chem 279:3318-3326. Pubmed: 14607838
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Haemig, H. A., Brooker, R. J. (2004). "Importance of conserved acidic residues in mntH, the Nramp homolog of Escherichia coli." J Membr Biol 201:97-107. Pubmed: 15630547
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kehres, D. G., Zaharik, M. L., Finlay, B. B., Maguire, M. E. (2000). "The NRAMP proteins of Salmonella typhimurium and Escherichia coli are selective manganese transporters involved in the response to reactive oxygen." Mol Microbiol 36:1085-1100. Pubmed: 10844693
  • Makui, H., Roig, E., Cole, S. T., Helmann, J. D., Gros, P., Cellier, M. F. (2000). "Identification of the Escherichia coli K-12 Nramp orthologue (MntH) as a selective divalent metal ion transporter." Mol Microbiol 35:1065-1078. Pubmed: 10712688
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837