Nicotinate-nucleotide adenylyltransferase (P0A752)

Identification
Name:Nicotinate-nucleotide adenylyltransferase
Synonyms:
  • Deamido-NAD(+) diphosphorylase
  • Deamido-NAD(+) pyrophosphorylase
  • Nicotinate mononucleotide adenylyltransferase
  • NaMN adenylyltransferase
Gene Name:nadD
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the reversible adenylation of nicotinate mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD)
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Nicotinamide ribotide + 1.0NMN ↔ 1.0NAD + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Nicotinamide ribotide ↔ 1.0Nicotinic acid adenine dinucleotide + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Nicotinamide ribotide ↔ 1.0Pyrophosphate + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Nicotinamide ribotide ↔ 1.0Pyrophosphate + 1.0Nicotinic acid adenine dinucleotide
ReactionCard
SMPDB Reactions:
1.0nicotinate beta-D-ribonucleotide+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0nicotinate beta-D-ribonucleotide + 1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Nicotinamide ribotide → 1.0Pyrophosphate + 1.0Nicotinic acid adenine dinucleotide
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Nicotinamide ribotide + 1.0NMN ↔ 1.0NAD + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0Nicotinamide ribotide ↔ 1.0Nicotinic acid adenine dinucleotide + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Nicotinamide ribotide ↔ 1.0Pyrophosphate + 1.0NAD
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Adenosine triphosphate + 1.0Nicotinamide ribotide → 1.0Pyrophosphate + 1.0Nicotinic acid adenine dinucleotide
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Nicotinamide ribotide → 1.0Pyrophosphate + 1.0Nicotinic acid adenine dinucleotide
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00902NADMetaboCard
ECMDB00229Nicotinamide ribotideMetaboCard
ECMDB01179Nicotinic acid adenine dinucleotideMetaboCard
ECMDB23222NMNMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
catalytic activity
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
cellular metabolic process
coenzyme biosynthetic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
NAD biosynthetic process
nicotinamide nucleotide biosynthetic process
pyridine nucleotide biosynthetic process
Gene Properties
Blattner:b0639
Gene OrientationCounterclockwise
Centisome Percentage:14.42
Left Sequence End669154
Right Sequence End669795
Gene Sequence:
>642 bp
ATGAAATCTTTACAGGCTCTGTTTGGCGGCACCTTTGATCCGGTGCACTATGGTCATCTA
AAACCCGTGGAAACGCTGGCGAATTTGATTGGTCTGACGCGGGTCACAATCATCCCTAAT
AATGTTCCTCCGCATCGTCCCCAGCCGGAAGCGAACAGCGTGCAGCGTAAACACATGCTT
GAACTGGCGATTGCCGACAAGCCATTATTTACTCTTGATGAACGCGAGCTAAAGCGCAAT
GCCCCCTCTTACACTGCGCAAACACTGAAAGAGTGGCGGCAGGAACAAGGACCGGACGTG
CCGCTGGCGTTTATTATTGGTCAGGATTCACTGCTGACCTTTCCGACCTGGTACGAATAC
GAAACGATACTCGACAATGCACATTTGATCGTCTGTCGGCGTCCAGGTTACCCACTTGAA
ATGGCGCAACCGCAATACCAGCAATGGCTGGAAGATCATTTGACACATAACCCGGAAGAT
CTTCACCTTCAGCCTGCCGGTAAAATTTATCTGGCTGAAACGCCGTGGTTTAACATCTCG
GCGACCATCATCCGCGAACGTTTGCAAAACGGTGAATCATGTGAGGATTTATTGCCGGAA
CCGGTACTGACTTACATTAACCAACAAGGCTTGTATCGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:213
Protein Molecular Weight:24528
Protein Theoretical pI:6
PDB File:1K4K
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Nicotinate-nucleotide adenylyltransferase
MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPPHRPQPEANSVQRKHML
ELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPLAFIIGQDSLLTFPTWYEY
ETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPEDLHLQPAGKIYLAETPWFNIS
ATIIRERLQNGESCEDLLPEPVLTYINQQGLYR
References
External Links:
ResourceLink
Uniprot ID:P0A752
Uniprot Name:NADD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062257
PDB ID:1K4K
Ecogene ID:EG13241
Ecocyc:EG13241
ColiBase:b0639
Kegg Gene:b0639
EchoBASE ID:EB3030
CCDB:NADD_ECOLI
BacMap:16128622
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Fountoulakis, M., Takacs, M. F., Berndt, P., Langen, H., Takacs, B. (1999). "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Electrophoresis 20:2181-2195. Pubmed: 10493123
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Mehl, R. A., Kinsland, C., Begley, T. P. (2000). "Identification of the Escherichia coli nicotinic acid mononucleotide adenylyltransferase gene." J Bacteriol 182:4372-4374. Pubmed: 10894752
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Zhang, H., Zhou, T., Kurnasov, O., Cheek, S., Grishin, N. V., Osterman, A. (2002). "Crystal structures of E. coli nicotinate mononucleotide adenylyltransferase and its complex with deamido-NAD." Structure 10:69-79. Pubmed: 11796112