Thiosulfate sulfurtransferase glpE (P0A6V5)

Identification
Name:Thiosulfate sulfurtransferase glpE
Synonyms:Not Available
Gene Name:glpE
Enzyme Class:
Biological Properties
General Function:Involved in thiosulfate sulfurtransferase activity
Specific Function:Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide. The relatively low affinity of glpE for both thiosulfate and cyanide suggests that these compounds are not the physiological substrates. Thioredoxin 1 or related dithiol proteins could instead be the physiological sulfur-acceptor substrate. Possible association with the metabolism of glycerol-phosphate remains to be elucidated
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Thiosulfate + 1.0Cyanide ↔ 1.0Sulfite + 1.0Thiocyanate
ReactionCard
SMPDB Reactions:
1.0Cyanide+1.0Thiosulfate+1.0Thumb+1.0Thumb → 1.0Thiocyanate+1.0Sulfite+1.0Thumb+1.0Thumb+1.0Thumb
1.0Cyanide + 1.0Thiosulfate + 1.0Cyanide + 1.0Thiosulfate → 1.0Thiocyanate + 1.0Sulfite + 1.0Hydrogen ion + 1.0Thiocyanate + 1.0Sulfite
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+2.0Thumb
1.0Hydrogen cyanide + 1.0Thiosulfate → 1.0Thiocyanate + 1.0Sulfite + 2.0Hydrogen ion
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Thiosulfate + 1.0Hydrogen cyanide → 1.0Sulfite + 1.0Thiocyanate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB02084CyanideMetaboCard
ECMDB21231Hydrogen cyanideMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00240SulfiteMetaboCard
ECMDB21289ThiocyanateMetaboCard
ECMDB00257ThiosulfateMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b3425
Gene OrientationCounterclockwise
Centisome Percentage:76.72
Left Sequence End3559520
Right Sequence End3559846
Gene Sequence:
>327 bp
ATGAAAAAACTGATTCTCATCGCCATAATGGCATCGGGGCTGGTAGCTTGTGCGCAATCA
ACCGCGCCACAGGAAGACAGTCGTCTGAAAGAGGCATACAGCGCCTGTATCAACACCGCA
CAAGGTTCGCCAGAAAAAATTGAAGCCTGCCAGAGCGTGTTAAACGTGCTGAAGAAAGAG
AAACAACATCAGCAGTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGT
CTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGG
CAGGAAATACGCAGTAATAACAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:108
Protein Molecular Weight:12082
Protein Theoretical pI:4
PDB File:1GN0
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Thiosulfate sulfurtransferase glpE
MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLTNDTLGAFMRDNDFDTP
VMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPAEVAYGA
References
External Links:
ResourceLink
Uniprot ID:P0A6V5
Uniprot Name:GLPE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062283
PDB ID:1GN0
Ecogene ID:EG10395
Ecocyc:EG10395
ColiBase:b3425
Kegg Gene:b3425
EchoBASE ID:EB0390
CCDB:GLPE_ECOLI
BacMap:16131299
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bordo, D., Larson, T. J., Donahue, J. L., Spallarossa, A., Bolognesi, M. (2000). "Crystals of GlpE, a 12 kDa sulfurtransferase from escherichia coli, display 1.06 A resolution diffraction: a preliminary report." Acta Crystallogr D Biol Crystallogr 56:1691-1693. Pubmed: 11092948
  • Cheng, H., Donahue, J. L., Battle, S. E., Ray, W. K., Larson, T. J. (2008). "Biochemical and Genetic Characterization of PspE and GlpE, Two Single-domain Sulfurtransferases of Escherichia coli." Open Microbiol J 2:18-28. Pubmed: 19088907
  • Choi, Y. L., Kawase, S., Kawamukai, M., Sakai, H., Komano, T. (1991). "Regulation of glpD and glpE gene expression by a cyclic AMP-cAMP receptor protein (cAMP-CRP) complex in Escherichia coli." Biochim Biophys Acta 1088:31-35. Pubmed: 1846566
  • Choi, Y. L., Kawase, S., Nishida, T., Sakai, H., Komano, T., Kawamukai, M., Utsumi, R., Kohara, Y., Akiyama, K. (1988). "Nucleotide sequence of the glpR gene encoding the repressor for the glycerol-3-phosphate regulon of Escherichia coli K12." Nucleic Acids Res 16:7732. Pubmed: 3045764
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ray, W. K., Zeng, G., Potters, M. B., Mansuri, A. M., Larson, T. J. (2000). "Characterization of a 12-kilodalton rhodanese encoded by glpE of Escherichia coli and its interaction with thioredoxin." J Bacteriol 182:2277-2284. Pubmed: 10735872
  • Spallarossa, A., Donahue, J. L., Larson, T. J., Bolognesi, M., Bordo, D. (2001). "Escherichia coli GlpE is a prototype sulfurtransferase for the single-domain rhodanese homology superfamily." Structure 9:1117-1125. Pubmed: 11709175
  • Zeng, G., Ye, S., Larson, T. J. (1996). "Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA-binding domain." J Bacteriol 178:7080-7089. Pubmed: 8955387