Galactose-1-phosphate uridylyltransferase (P09148)

Identification
Name:Galactose-1-phosphate uridylyltransferase
Synonyms:
  • Gal-1-P uridylyltransferase
  • UDP-glucose--hexose-1-phosphate uridylyltransferase
Gene Name:galT
Enzyme Class:
Biological Properties
General Function:Involved in UDP-glucose:hexose-1-phosphate uridylyltransferase activity
Specific Function:UDP-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-galactose
Cellular Location:Not Available
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism II PW000887
  • Amino sugar and nucleotide sugar metabolism III PW000895
  • Galactose metabolism PW000821
  • Starch and sucrose metabolism PW000941
  • colanic acid building blocks biosynthesis PW000951
  • galactose degradation/Leloir Pathway PW000884
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Galactose 1-phosphate + 1.0UDP-Glucose ↔ 1.0Glucose 1-phosphate + 1.0Uridine diphosphategalactose
ReactionCard
SMPDB Reactions:
1.0Galactose 1-phosphate+1.0Thumb → 1.0Thumb
1.0Galactose 1-phosphate + 1.0Galactose 1-phosphate → 1.0Glucose 1-phosphate
ReactionCard
1.0Thumb → 1.0Uridine diphosphategalactose+1.0Thumb
1.0UDP-Glucose → 1.0Uridine diphosphategalactose + 1.0Uridine diphosphategalactose
ReactionCard
1.0Galactose 1-phosphate+1.0Thumb+1.0Thumb → 1.0Uridine diphosphategalactose+1.0Thumb+1.0Thumb
1.0Galactose 1-phosphate + 1.0UDP-Glucose + 1.0Galactose 1-phosphate → 1.0Uridine diphosphategalactose + 1.0Glucose 1-phosphate + 1.0Uridine diphosphategalactose
ReactionCard
1.0Alpha-D-glucose 1-phosphate+1.0UDP-galactose → 1.0Thumb+1.0Galactose 1-phosphate+1.0Thumb
1.0Alpha-D-glucose 1-phosphate + 1.0UDP-galactose → 1.0UDP-Glucose + 1.0Galactose 1-phosphate + 1.0Galactose 1-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Galactose 1-phosphate + 1.0UDP-Glucose ↔ 1.0Glucose 1-phosphate + 1.0Uridine diphosphategalactose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04011Galactose 1-phosphateMetaboCard
ECMDB01586Glucose 1-phosphateMetaboCard
ECMDB04171UDP-GlucoseMetaboCard
ECMDB00302Uridine diphosphategalactoseMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ion binding
metal ion binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transition metal ion binding
UDP-glucose:hexose-1-phosphate uridylyltransferase activity
uridylyltransferase activity
zinc ion binding
Process
alcohol metabolic process
galactose metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Blattner:b0758
Gene OrientationCounterclockwise
Centisome Percentage:17.01
Left Sequence End789206
Right Sequence End790252
Gene Sequence:
>1047 bp
ATGACGCAATTTAATCCCGTTGATCATCCACATCGCCGCTACAACCCGCTCACCGGGCAA
TGGATTCTGGTTTCACCGCACCGCGCTAAGCGCCCCTGGCAGGGGGCGCAGGAAACGCCA
GCCAAACAGGTGTTACCTGCGCACGATCCAGATTGCTTCCTCTGCGCAGGTAATGTGCGG
GTGACAGGCGATAAAAACCCCGATTACACCGGGACTTACGTTTTCACTAATGACTTTGCG
GCTTTGATGTCTGACACGCCAGATGCGCCAGAAAGTCACGATCCGCTGATGCGTTGCCAG
AGCGCGCGCGGCACCAGCCGGGTGATCTGCTTTTCACCGGATCACAGTAAAACGCTGCCA
GAGCTCAGCGTTGCAGCATTGACGGAAATCGTCAAAACCTGGCAGGAGCAAACCGCAGAA
CTGGGGAAAACGTACCCATGGGTGCAGGTTTTTGAAAACAAAGGCGCGGCGATGGGCTGC
TCTAACCCGCATCCGCACGGTCAGATTTGGGCAAATAGCTTCCTGCCTAACGAAGCTGAG
CGCGAAGACCGCCTGCAAAAAGAATATTTTGCCGAACAGAAATCACCAATGCTGGTGGAT
TATGTTCAGCGCGAGCTGGCAGACGGTAGCCGTACCGTTGTCGAAACCGAACACTGGTTA
GCCGTCGTGCCTTACTGGGCTGCCTGGCCGTTCGAAACGCTACTGCTGCCCAAAGCCCAC
GTTTTACGGATCACCGATTTGACCGACGCCCAGCGCAGCGATCTGGCGCTGGCGTTGAAA
AAGCTGACCAGTCGTTATGACAACCTCTTCCAGTGCTCCTTCCCCTACTCTATGGGCTGG
CACGGCGCGCCATTTAATGGCGAAGAGAATCAACACTGGCAGCTGCACGCGCACTTTTAT
CCGCCTCTGCTGCGCTCCGCCACCGTACGTAAATTTATGGTTGGTTATGAAATGCTGGCA
GAGACCCAGCGAGACCTGACCGCAGAACAGGCAGCAGAGCGTTTGCGCGCAGTCAGCGAT
ATCCATTTTCGCGAATCCGGAGTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:348
Protein Molecular Weight:39645
Protein Theoretical pI:6
PDB File:1HXQ
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Galactose-1-phosphate uridylyltransferase
MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQVLPAHDPDCFLCAGNVR
VTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSARGTSRVICFSPDHSKTLP
ELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGCSNPHPHGQIWANSFLPNEAE
REDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEHWLAVVPYWAAWPFETLLLPKAH
VLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPYSMGWHGAPFNGEENQHWQLHAHFY
PPLLRSATVRKFMVGYEMLAETQRDLTAEQAAERLRAVSDIHFRESGV
References
External Links:
ResourceLink
Uniprot ID:P09148
Uniprot Name:GAL7_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651343
PDB ID:1HXQ
Ecogene ID:EG10366
Ecocyc:EG10366
ColiBase:b0758
Kegg Gene:b0758
EchoBASE ID:EB0361
CCDB:GAL7_ECOLI
BacMap:16128726
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cornwell, T. L., Adhya, S. L., Reznikoff, W. S., Frey, P. A. (1987). "The nucleotide sequence of the gal T gene of Escherichia coli." Nucleic Acids Res 15:8116. Pubmed: 2823224
  • Debouck, C., Riccio, A., Schumperli, D., McKenney, K., Jeffers, J., Hughes, C., Rosenberg, M., Heusterspreute, M., Brunel, F., Davison, J. (1985). "Structure of the galactokinase gene of Escherichia coli, the last (?) gene of the gal operon." Nucleic Acids Res 13:1841-1853. Pubmed: 3158881
  • Geeganage, S., Ling, V. W., Frey, P. A. (2000). "Roles of two conserved amino acid residues in the active site of galactose-1-phosphate uridylyltransferase: an essential serine and a nonessential cysteine." Biochemistry 39:5397-5404. Pubmed: 10820011
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lemaire, H. G., Muller-Hill, B. (1986). "Nucleotide sequences of the gal E gene and the gal T gene of E. coli." Nucleic Acids Res 14:7705-7711. Pubmed: 3022232
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Thoden, J. B., Ruzicka, F. J., Frey, P. A., Rayment, I., Holden, H. M. (1997). "Structural analysis of the H166G site-directed mutant of galactose-1-phosphate uridylyltransferase complexed with either UDP-glucose or UDP-galactose: detailed description of the nucleotide sugar binding site." Biochemistry 36:1212-1222. Pubmed: 9063869
  • Wedekind, J. E., Frey, P. A., Rayment, I. (1995). "Three-dimensional structure of galactose-1-phosphate uridylyltransferase from Escherichia coli at 1.8 A resolution." Biochemistry 34:11049-11061. Pubmed: 7669762
  • Wedekind, J. E., Frey, P. A., Rayment, I. (1996). "The structure of nucleotidylated histidine-166 of galactose-1-phosphate uridylyltransferase provides insight into phosphoryl group transfer." Biochemistry 35:11560-11569. Pubmed: 8794735
  • Wong, L. J., Sheu, K. F., Lee, S. L., Frey, P. A. (1977). "Galactose-1-phosphate uridylyltransferase: isolation and properties of a uridylyl-enzyme intermediate." Biochemistry 16:1010-1016. Pubmed: 321007