Galactoside O-acetyltransferase (P07464)

Identification
Name:Galactoside O-acetyltransferase
Synonyms:
  • GAT
  • Thiogalactoside acetyltransferase
Gene Name:lacA
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity
Specific Function:May assist cellular detoxification by acetylating non- metabolizable pyranosides, thereby preventing their reentry into the cell
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0beta-D-Galactoside ↔ 1.0Thumb+1.06-Acetyl-beta-D-galactoside
1.0Acetyl-CoA + 1.0beta-D-Galactoside ↔ 1.0Coenzyme A + 1.06-Acetyl-beta-D-galactoside
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01206Acetyl-CoAMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
Gene Properties
Blattner:b0342
Gene OrientationCounterclockwise
Centisome Percentage:7.77
Left Sequence End360473
Right Sequence End361084
Gene Sequence:
>612 bp
TTGAACATGCCAATGACCGAAAGAATAAGAGCAGGCAAGCTATTTACCGATATGTGCGAA
GGCTTACCGGAAAAAAGACTTCGTGGGAAAACGTTAATGTATGAGTTTAATCACTCGCAT
CCATCAGAAGTTGAAAAAAGAGAAAGCCTGATTAAAGAAATGTTTGCCACGGTAGGGGAA
AACGCCTGGGTAGAACCGCCTGTCTATTTCTCTTACGGTTCCAACATCCATATAGGCCGC
AATTTTTATGCAAATTTCAATTTAACCATTGTCGATGACTACACGGTAACAATCGGTGAT
AACGTACTGATTGCACCCAACGTTACTCTTTCCGTTACGGGACACCCTGTACACCATGAA
TTGAGAAAAAACGGCGAGATGTACTCTTTTCCGATAACGATTGGCAATAACGTCTGGATC
GGAAGTCATGTGGTTATTAATCCAGGCGTCACCATCGGGGATAATTCTGTTATTGGCGCG
GGTAGTATCGTCACAAAAGACATTCCACCAAACGTCGTGGCGGCTGGCGTTCCTTGTCGG
GTTATTCGCGAAATAAACGACCGGGATAAGCACTATTATTTCAAAGATTATAAAGTTGAA
TCGTCAGTTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:203
Protein Molecular Weight:22799
Protein Theoretical pI:7
PDB File:1KRV
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Galactoside O-acetyltransferase
MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVEKRESLIKEMFATVGE
NAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLIAPNVTLSVTGHPVHHE
LRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSIVTKDIPPNVVAAGVPCR
VIREINDRDKHYYFKDYKVESSV
References
External Links:
ResourceLink
Uniprot ID:P07464
Uniprot Name:THGA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674484
PDB ID:1KRV
Ecogene ID:EG10524
Ecocyc:EG10524
ColiBase:b0342
Kegg Gene:b0342
EchoBASE ID:EB0519
CCDB:THGA_ECOLI
BacMap:16128327
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Buchel, D. E., Gronenborn, B., Muller-Hill, B. (1980). "Sequence of the lactose permease gene." Nature 283:541-545. Pubmed: 6444453
  • Fowler, A. V., Hediger, M. A., Musso, R. E., Zabin, I. (1985). "The amino acid sequence of thiogalactoside transacetylase of Escherichia coli." Biochimie 67:101-108. Pubmed: 3922433
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hediger, M. A., Johnson, D. F., Nierlich, D. P., Zabin, I. (1985). "DNA sequence of the lactose operon: the lacA gene and the transcriptional termination region." Proc Natl Acad Sci U S A 82:6414-6418. Pubmed: 3901000
  • Wang, X. G., Olsen, L. R., Roderick, S. L. (2002). "Structure of the lac operon galactoside acetyltransferase." Structure 10:581-588. Pubmed: 11937062