Sodium/proline symporter (P07117)

Identification
Name:Sodium/proline symporter
Synonyms:
  • Proline permease
  • Propionate transporter
Gene Name:putP
Enzyme Class:Not Available
Biological Properties
General Function:Involved in proline:sodium symporter activity
Specific Function:Catalyzes the sodium-dependent uptake of extracellular L-proline. This protein is also capable of using lithium as the transport cation. Also catalyzes the uptake of propionate
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB00162L-ProlineMetaboCard
ECMDB21393LithiumMetaboCard
ECMDB00237Propionic acidMetaboCard
ECMDB02908SodiumMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
alkali metal ion binding
binding
cation binding
ion binding
metal ion binding
organic acid transmembrane transporter activity
organic acid:sodium symporter activity
proline:sodium symporter activity
sodium ion binding
sodium:amino acid symporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
amine transport
amino acid transport
cation transport
establishment of localization
ion transport
monovalent inorganic cation transport
neutral amino acid transport
proline transport
sodium ion transport
transmembrane transport
transport
Gene Properties
Blattner:b1015
Gene OrientationClockwise
Centisome Percentage:23.25
Left Sequence End1078528
Right Sequence End1080036
Gene Sequence:
>1509 bp
ATGGCTATTAGCACACCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTG
ATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGT
AGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTG
TTAATGGGGTTGCCGGGCGCTGTTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATT
GGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCAT
ACCGAATACAACAATAACGCCTTAACACTGCCGGATTATTTCACCGGGCGCTTTGAAGAT
AAAAGCCGCATTTTGCGCATTATCTCTGCGCTGGTTATTTTGCTGTTCTTCACCATTTAT
TGCGCTTCGGGCATTGTGGCAGGCGCGCGTCTGTTTGAAAGTACCTTTGGCATGAGCTAC
GAAACGGCTCTGTGGGCGGGCGCTGCGGCGACGATCCTTTACACCTTTATTGGCGGTTTC
CTCGCGGTGAGCTGGACTGACACTGTACAGGCCAGCCTGATGATTTTTGCCCTGATCCTG
ACGCCGGTTATCGTCATTATCAGTGTCGGTGGCTTTGGTGACTCGCTGGAAGTGATCAAA
CAAAAGAGCATCGAAAACGTTGATATGCTCAAAGGTCTGAACTTTGTTGCCATTATCTCA
CTGATGGGTTGGGGGCTGGGTTACTTCGGGCAGCCGCACATTCTGGCGCGTTTTATGGCG
GCGGATTCTCACCACAGCATTGTCCATGCGCGTCGTATTAGTATGACCTGGATGATCCTC
TGCCTGGCAGGGGCGGTGGCTGTCGGCTTCTTTGGGATTGCTTACTTTAACGATCATCCG
GCGTTGGCTGGTGCGGTAAATCAGAACGCCGAGCGTGTGTTTATCGAACTGGCGCAAATT
CTGTTTAACCCGTGGATTGCCGGGATTCTGCTGTCGGCAATTCTGGCGGCGGTAATGTCA
ACCTTAAGTTGCCAGCTGCTGGTGTGCTCCAGTGCGATTACCGAAGATTTGTACAAAGCG
TTTCTGCGTAAACATGCCAGCCAGAAAGAGCTGGTGTGGGTAGGGCGTGTGATGGTGCTG
GTGGTGGCGCTGGTGGCGATTGCGCTGGCGGCAAACCCGGAAAACCGCGTGCTGGGCTTA
GTGAGCTACGCGTGGGCAGGCTTTGGCGCGGCGTTTGGTCCAGTGGTGCTGTTCTCGGTG
ATGTGGTCACGCATGACGCGTAACGGTGCGCTGGCGGGGATGATCATCGGTGCGCTGACG
GTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATTATTCCGGGCTTTATC
TTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCCGTCAGCGGCGATG
CAAAAACGCTTTGCCGAGGCCGATGCGCACTATCATTCGGCTCCGCCGTCACGGTTGCAG
GAAAGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:502
Protein Molecular Weight:54344
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 4-26
  • 67-87
  • 125-150
  • 163-184
  • 190-213
  • 232-248
  • 275-295
  • 320-340
  • 371-391
  • 398-423
  • 431-445
  • 449-475
Protein Sequence:
>Sodium/proline symporter
MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGPFVTALSAGASDMSGWL
LMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTEYNNNALTLPDYFTGRFED
KSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSYETALWAGAAATILYTFIGGF
LAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEVIKQKSIENVDMLKGLNFVAIIS
LMGWGLGYFGQPHILARFMAADSHHSIVHARRISMTWMILCLAGAVAVGFFGIAYFNDHP
ALAGAVNQNAERVFIELAQILFNPWIAGILLSAILAAVMSTLSCQLLVCSSAITEDLYKA
FLRKHASQKELVWVGRVMVLVVALVAIALAANPENRVLGLVSYAWAGFGAAFGPVVLFSV
MWSRMTRNGALAGMIIGALTVIVWKQFGWLGLYEIIPGFIFGSIGIVVFSLLGKAPSAAM
QKRFAEADAHYHSAPPSRLQES
References
External Links:
ResourceLink
Uniprot ID:P07117
Uniprot Name:PUTP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651504
Ecogene ID:EG10802
Ecocyc:EG10802
ColiBase:b1015
Kegg Gene:b1015
EchoBASE ID:EB0795
CCDB:PUTP_ECOLI
BacMap:16128981
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hanada, K., Yoshida, T., Yamato, I., Anraku, Y. (1992). "Sodium ion and proline binding sites in the Na+/proline symport carrier of Escherichia coli." Biochim Biophys Acta 1105:61-66. Pubmed: 1567896
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Nakao, T., Yamato, I., Anraku, Y. (1987). "Nucleotide sequence of putC, the regulatory region for the put regulon of Escherichia coli K12." Mol Gen Genet 210:364-368. Pubmed: 3325781
  • Nakao, T., Yamato, I., Anraku, Y. (1987). "Nucleotide sequence of putP, the proline carrier gene of Escherichia coli K12." Mol Gen Genet 208:70-75. Pubmed: 3302614
  • Nelson, K., Selander, R. K. (1992). "Evolutionary genetics of the proline permease gene (putP) and the control region of the proline utilization operon in populations of Salmonella and Escherichia coli." J Bacteriol 174:6886-6895. Pubmed: 1400239
  • Ohsawa, M., Mogi, T., Yamamoto, H., Yamato, I., Anraku, Y. (1988). "Proline carrier mutant of Escherichia coli K-12 with altered cation sensitivity of substrate-binding activity: cloning, biochemical characterization, and identification of the mutation." J Bacteriol 170:5185-5191. Pubmed: 3053649
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Reed, J. L., Patel, T. R., Chen, K. H., Joyce, A. R., Applebee, M. K., Herring, C. D., Bui, O. T., Knight, E. M., Fong, S. S., Palsson, B. O. (2006). "Systems approach to refining genome annotation." Proc Natl Acad Sci U S A 103:17480-17484. Pubmed: 17088549
  • Stenberg, F., Chovanec, P., Maslen, S. L., Robinson, C. V., Ilag, L. L., von Heijne, G., Daley, D. O. (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280:34409-34419. Pubmed: 16079137
  • Yamato, I., Anraku, Y. (1988). "Site-specific alteration of cysteine 281, cysteine 344, and cysteine 349 in the proline carrier of Escherichia coli." J Biol Chem 263:16055-16057. Pubmed: 3053687
  • Yamato, I., Kotani, M., Oka, Y., Anraku, Y. (1994). "Site-specific alteration of arginine 376, the unique positively charged amino acid residue in the mid-membrane-spanning regions of the proline carrier of Escherichia coli." J Biol Chem 269:5720-5724. Pubmed: 8119910