Melibiose carrier protein (P02921)

Identification
Name:Melibiose carrier protein
Synonyms:
  • Melibiose permease
  • Melibiose transporter
  • Na+ (Li+)/melibiose symporter
  • Thiomethylgalactoside permease II
Gene Name:melB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Responsible for melibiose transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system)
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB04025beta-D-GalactoseMetaboCard
ECMDB01362Hydrogen (gas)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21393LithiumMetaboCard
ECMDB00048MelibioseMetaboCard
ECMDB21382Methyl-beta-D-galactosideMetaboCard
ECMDB02908SodiumMetaboCard
ECMDB21378ThiomethylgalactosideMetaboCard
ECMDB00302Uridine diphosphategalactoseMetaboCard
GO Classification:
Component
cell part
membrane
Function
transporter activity
Process
cation transport
establishment of localization
ion transport
monovalent inorganic cation transport
sodium ion transport
transport
Gene Properties
Blattner:b4120
Gene OrientationClockwise
Centisome Percentage:93.57
Left Sequence End4341392
Right Sequence End4342813
Gene Sequence:
>1410 bp
ATGTTCAATGGTCGTCCTTTCCCTGTAGATGCATTTCCTAAAATTATCAGGAATGCAATT
TATGAAGTGGAACAGCATACGCAGGCCCCTCAAGGTTTGATTGCTGCTTCTGCTCTTGGG
GTAATTTCTCTTGCCTGTCAGAACCGGATTGATGTTTGCCGATTGAATAATCTACGTGGC
CCGGTATCACTTTTCTTAATGACTCTGGCTGAATCAGGTGAACGTAAGAGTACGGTTGAT
AAACTGCTGATGAAGCCATTATATCAACTGGAAGAGGATTTATTTGAAAAATACACCCAC
GATCTTACCGCATGGAGAAATGATGAAGCAATTTTTAATATTGAAAAAAAAGCACTGATG
TCAAAACTTAAATCAGATATTCGACGTAACAAAGATCACTTGGCAACAAATGAAAGACTT
AAAGAACTACTTACGACAAACCCGAAAGCTCCAGTGAGATTCAAATTTTTATTTAACGAT
GCCACACCTGCAGCTATTAAAGCTCATCTCTGTGGGCACTGGCGATCAGTCGGCATCATG
TCTGATGAAGCTGGGATCATTTTTAATGGTTACACACTTAACGAGCTGCCGTTTATCAAT
AAGATGTGGGATGGTTCAATATTTACGGTGGAAAGGAAAAACGAGCCCGAGAAATTAATT
AGAGATGCAAGAATAACACTGTCGCTGATGGTCCAGCCTAATGTTTTTAAGGGTTATATC
GACAGGAAAGGAGATATGGCAAAGGGGATTGGATTTTTTGCACGGTGCCTCATGTGCCAG
CCTGCTTCAACACAAGGTAACAGAAAAATTTCCAACCCAATTTTTTCAAATGAACATTTG
CCGGTATTTCACCAACGTCTTATGGAAATTGTTAATGAGAGCATCATTAAAATTAATGAA
AATAATCGCATCTGCCTCCGATTCTCTGCAGAAGCAGAAAGACATTGGATCGAATTCTAC
AACCAGGTCGAGTCAGAAATGAGAATGATTGGCCTTCTTTATGATTTTAAGGATTATGCT
TCTAAAATGGCGGAGAACATGGCGAGGCTTGCTGCCTTACTTCATTACTTCAGCGGTGAT
GGAGGCGATATATCTGTTACCGCAGTAAAAGCAGCAGTGGAGATAGTGGCTTGGTATATT
GAAGAATACATCCGCTTGTTCTCTAAAAAAGAAGAGTTTTCTTTAGATGTTTCAGAAGCA
GATGAGCTTTATTGTTGGATAAAAGATTACTGCACGCAAAAATTTTCTTCCTGCATCAAG
AAAAATATTATCTTACAATTTGGGCCAAATAAATTTAGAAATCGTGACAAGGCAAATGAA
TTAATTAGAATCTTAATTTCACAAAACAAAATATTTATATCTTCATGGGGTAAAACAAAA
ATAATAAACATAACTCATTGTGTTTTTTGA
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:469
Protein Molecular Weight:52217
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 16-36
  • 39-59
  • 77-97
  • 105-125
  • 150-170
  • 183-203
  • 232-252
  • 266-286
  • 296-316
  • 325-345
  • 378-398
  • 407-427
Protein Sequence:
>Melibiose carrier protein
MTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLVGTLFLVARIWDAINDPIMGW
IVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEGTTQIVFVCVTYILWGMTYTIMD
IPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTAGVTLPFVNYVGGGDRGFGFQMFTL
VLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHLTLKAIVALIYKNDQLSCLLGMALAYN
VASNIITGFAIYYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILWAGASI
LPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADIVDYGEYKLHVRC
ESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMV
TLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA
References
External Links:
ResourceLink
Uniprot ID:P02921
Uniprot Name:MELB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675488
Ecogene ID:EG10578
Ecocyc:EG10578
ColiBase:b4120
Kegg Gene:b4120
EchoBASE ID:EB0573
CCDB:MELB_ECOLI
BacMap:16131946
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Botfield, M. C., Naguchi, K., Tsuchiya, T., Wilson, T. H. (1992). "Membrane topology of the melibiose carrier of Escherichia coli." J Biol Chem 267:1818-1822. Pubmed: 1730719
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pourcher, T., Bibi, E., Kaback, H. R., Leblanc, G. (1996). "Membrane topology of the melibiose permease of Escherichia coli studied by melB-phoA fusion analysis." Biochemistry 35:4161-4168. Pubmed: 8672452
  • Pourcher, T., Deckert, M., Bassilana, M., Leblanc, G. (1991). "Melibiose permease of Escherichia coli: mutation of aspartic acid 55 in putative helix II abolishes activation of sugar binding by Na+ ions." Biochem Biophys Res Commun 178:1176-1181. Pubmed: 1872836
  • Yazyu, H., Shiota-Niiya, S., Shimamoto, T., Kanazawa, H., Futai, M., Tsuchiya, T. (1984). "Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli." J Biol Chem 259:4320-4326. Pubmed: 6323466