Displaying growth conditions 801 - 825 of 1187 in total
ECMDB IDNameConcentration Strain
Media
Growth Status
Growth System
TemperatureCitation
ECMDB00158L-Tyrosine29± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB01276N1-Acetylspermidine30± 0 uMK12 HB101
199 Medium with Earle’s salts –which contains 21 amino acids, 17 vitamins, 10 components of nucleic acids, sodium acetate, glucose, NaC1, KCl, CaC12, MgS04, Na2HP04, and Fe(N03)3
Stationary Phase
Shake flask
37 oCPMID: 8742354
ECMDB00223Oxalacetic acid30± 1 uMBW25113
M9 Minimal Media, 4 g/L Glucose
Mid-Log Phase
Bioreactor, pH controlled, O2 controlled, dilution rate: 0.2/h
37 oCPMID: 15158257
ECMDB01201Guanosine diphosphate30± 2 uMK12
0.2 g/L NH4Cl, 2.0 g/L (NH4)2SO4, 3.25 g/L KH2PO4, 2.5 g/L K2HPO4, 1.5 g/L NaH2PO4, 0.5 g/L MgSO4; trace substances: 10 mg/L CaCl2, 0.5 mg/L ZnSO4, 0.25 mg/L CuCl2, 0.25 mg/L MnSO4, 0.175 mg/L CoCl2, 0.125 mg/L H3BO3, 2.5 mg/L AlCl3, 0.5 mg/L Na2MoO4, 10
Stationary Phase, glucose limited
Bioreactor, pH controlled, aerated, dilution rate=0.125 L/h
37 oCPMID: 11488613
ECMDB00214Ornithine30± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00696L-Methionine30± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00195Inosine31± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00271Sarcosine32± 2 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
ECMDB214074-Oxohexanoate32± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB214042,4-Diaminobutyric acid33± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00172L-Isoleucine33± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00271Sarcosine33± 6 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
ECMDB01586Glucose 1-phosphate33± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00034Adenine34± 10 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
ECMDB00998dCTP34± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00058Cyclic AMP35± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB01175Malonyl-CoA35± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00060Acetoacetic acid37± 9 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
ECMDB01197FADH238± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00158L-Tyrosine38± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00904Citrulline38± 0 uMBW25113
48 mM Na2HPO4, 22 mM KH2PO4, 10 mM NaCl, 45 mM (NH4)2SO4, supplemented with 1 mM MgSO4, 1 mg/l thiamine·HCl, 5.6 mg/l CaCl2, 8 mg/l FeCl3, 1 mg/l MnCl2·4H2O, 1.7 mg/l ZnCl2, 0.43 mg/l CuCl2·2H2O, 0.6 mg/l CoCl2·2H2O and 0.6 mg/l Na2MoO4·2H2O. 4 g/L Gluco
Stationary Phase, glucose limited
Bioreactor, pH controlled, O2 and CO2 controlled, dilution rate: 0.2/h
37 oCPMID: 17379776
ECMDB00625Gluconic acid42± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00159L-Phenylalanine42± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glycerol
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
ECMDB00696L-Methionine43± 5 uMBL21 DE3
Luria-Bertani (LB) media
Stationary phase cultures (overnight culture)
Shake flask
37 oCExperimentally Determined
Download Details
ECMDB03357N-Acetylornithine43± 0 uMK12 NCM3722
Gutnick minimal complete medium (4.7 g/L KH2PO4; 13.5 g/L K2HPO4; 1 g/L K2SO4; 0.1 g/L MgSO4-7H2O; 10 mM NH4Cl) with 4 g/L glucose
Mid-Log Phase
Shake flask and filter culture
37 oCPMID: 19561621
Displaying growth conditions 801 - 825 of 1187 in total