2.02012-05-31 13:44:18 -06002015-09-17 16:24:05 -0600ECMDB01060M2MDB000237Ubiquinol-8Ubiquinol-8 is a member of the chemical class known as Polyprenylbenzoquinols. They are reduced forms of polyprenylbenzoquinines (ubiquinones). These are compounds containing a polyisoprene chain attached to a quinol at the second ring position. Ubiquiol-1 has 8 isoprene units. Normally in E. coli the active form of Ubiquinol has 8 isoprene units (Ubiquinol-8) and in humans it normally has 10. Coenzyme Q(n) exists in three redox states, fully oxidized (ubiquinone), partially reduced (semiquinones or ubisemiquinones), and fully reduced (ubiquinols). The redox functions of ubiquinol in cellular energy production and antioxidant protection are based on the ability to exchange two electrons in a redox cycle between ubiquinol (reduced) and the ubiquinone (oxidized) form. Ubiquionols are important in cellular respiration. They are fat-soluble and therefore mobile in cellular membranes; they play a unique role in the electron transport chain (ETC). In the inner bacterial membrane, electrons from NADH and succinate pass through the ETC to the oxygen, which is then reduced to water. The transfer of electrons through ETC results in the pumping of H+ across the membrane creating a proton gradient across the membrane, which is used by ATP synthase (located on the membrane) to generate ATP.2,3-dimethoxy-5-methyl-6-(2E,6E,10E,14E,18E,22E,26E)-3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaenylbenzene-1,4-diolDihydroubiquinoneReduced coenzyme Q8Reduced ubiquinoneUbiquinol(30)Ubiquinol(8)C49H78O4731.1412730.5900109842,3-dimethoxy-5-methyl-6-(3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaen-1-yl)cyclohexa-2,5-diene-1,4-diol2,3-dimethoxy-5-methyl-6-(3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaen-1-yl)cyclohexa-2,5-diene-1,4-diolCOC1=C(OC)C(O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1OInChI=1S/C49H78O4/c1-36(2)20-13-21-37(3)22-14-23-38(4)24-15-25-39(5)26-16-27-40(6)28-17-29-41(7)30-18-31-42(8)32-19-33-43(9)34-35-45-44(10)46(50)48(52-11)49(53-12)47(45)51/h20,22,24,26,28,30,32,34,46-47,50-51H,13-19,21,23,25,27,29,31,33,35H2,1-12H3/b37-22+,38-24+,39-26+,40-28+,41-30+,42-32+,43-34+FLVUMORHBJZINO-SGHXUWJISA-NMembranelogp8.66logs-6.33solubility3.45e-04 g/llogp12pka_strongest_acidic12.91pka_strongest_basic-3.6iupac2,3-dimethoxy-5-methyl-6-(3,7,11,15,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaen-1-yl)cyclohexa-2,5-diene-1,4-diolaverage_mass731.1412mono_mass730.590010984smilesCOC1=C(OC)C(O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1OformulaC49H78O4inchiInChI=1S/C49H78O4/c1-36(2)20-13-21-37(3)22-14-23-38(4)24-15-25-39(5)26-16-27-40(6)28-17-29-41(7)30-18-31-42(8)32-19-33-43(9)34-35-45-44(10)46(50)48(52-11)49(53-12)47(45)51/h20,22,24,26,28,30,32,34,46-47,50-51H,13-19,21,23,25,27,29,31,33,35H2,1-12H3/b37-22+,38-24+,39-26+,40-28+,41-30+,42-32+,43-34+inchikeyFLVUMORHBJZINO-SGHXUWJISA-Npolar_surface_area58.92refractivity240.23polarizability93.43rotatable_bond_count25acceptor_count4donor_count2physiological_charge0formal_charge0Pentose phosphate pathwayec00030Oxidative phosphorylationThe process of oxidative phosphorylation involves multiple interactions of ubiquinone with succinic acid, resulting in a fumaric acid and ubiquinol.
Ubiquinone interacts with succinic acid through a succinate:quinone oxidoreductase resulting in a fumaric acid an ubiquinol. This enzyme has various cofactors, ferroheme b, 2FE-2S, FAD, and 3Fe-4S iron-sulfur cluster.
Then 2 ubiquinol interact with oxygen and 4 hydrogen ion through a cytochrome bd-I terminal oxidase resulting in a 4 hydrogen ion transferred into the periplasmic space, 2 water returned into the cytoplasm and 2 ubiquinone, which stay in the inner membrane.
The ubiquinone interacts with succinic acid through a succinate:quinone oxidoreductase resulting in a fumaric acid an ubiquinol.
Then 2 ubiquinol interacts with oxygen and 4 hydrogen ion through a cytochrome bd-II terminal oxidase resulting in a 4 hydrogen ion transferred into the periplasmic space, 2 water returned into the cytoplasm and 2 ubiquinone, which stay in the inner membrane.
The ubiquinone interacts with succinic acid through a succinate:quinone oxidoreductase resulting in a fumaric acid an ubiquinol.
The 2 ubiquinol interact with oxygen and 8 hydrogen ion through a cytochrome bo terminal oxidase resulting in a 8 hydrogen ion transferred into the periplasmic space, 2 water returned into the cytoplasm and 2 ubiquinone, which stays in the inner membrane.
The ubiquinone then interacts with 5 hydrogen ion through a NADH dependent ubiquinone oxidoreductase I resulting in NAD, hydrogen ion released into the periplasmic space and an ubiquinol.
The ubiquinol is then processed reacting with oxygen, and 4 hydrogen through a ion cytochrome bd-I terminal oxidase resulting in 4 hydrogen ions released into the periplasmic space, 2 water molecules into the cytoplasm and 2 ubiquinones.
The ubiquinone then interacts with 5 hydrogen ion through a NADH dependent ubiquinone oxidoreductase I resulting in NAD, hydrogen ion released into the periplasmic space and an ubiquinol.
The 2 ubiquinol interact with oxygen and 8 hydrogen ion through a cytochrome bo terminal oxidase resulting in a 8 hydrogen ion transferred into the periplasmic space, 2 water returned into the cytoplasm and 2 ubiquinone, which stays in the inner membrane.
PW000919ec00190MetabolicFructose and mannose metabolismec00051Phosphotransferase system (PTS)ec02060ABC transportersec02010Secondary Metabolites: Ubiquinol biosynthesisThe biosynthesis of ubiquinol starts the interaction of 4-hydroxybenzoic acid interacting with an octaprenyl diphosphate. The former compound comes from the chorismate interacting with a chorismate lyase resulting in the release of a pyruvic acid and a 4-hydroxybenzoic acid. On the other hand, the latter compound, octaprenyl diphosphate is the result of a farnesyl pyrophosphate interacting with an isopentenyl pyrophosphate through an octaprenyl diphosphate synthase resulting in the release of a pyrophosphate and an octaprenyl diphosphate.
The 4-hydroxybenzoic acid interacts with octaprenyl diphosphate through a 4-hydroxybenzoate octaprenyltransferase resulting in the release of a pyrophosphate and a 3-octaprenyl-4-hydroxybenzoate. The latter compound then interacts with a hydrogen ion through a 3-octaprenyl-4-hydroxybenzoate carboxy-lyase resulting in the release of a carbon dioxide and a 2-octaprenylphenol. The latter compound interacts with an oxygen molecule and a hydrogen ion through a NADPH driven 2-octaprenylphenol hydroxylase resulting in a NADP, a water molecule and a 2-octaprenyl-6-hydroxyphenol.
The 2-octaprenyl-6-hydroxyphenol interacts with an S-adenosylmethionine through a bifunctional 3-demethylubiquinone-8 3-O-methyltransferase and 2-octaprenyl-6-hydroxyphenol methylase resulting in the release of a hydrogen ion, an s-adenosylhomocysteine and a 2-methoxy-6-(all-trans-octaprenyl)phenol. The latter compound then interacts with an oxygen molecule and a hydrogen ion through a NADPH driven 2-octaprenyl-6-methoxyphenol hydroxylase resulting in a NADP, a water molecule and a 2-methoxy-6-all trans-octaprenyl-2-methoxy-1,4-benzoquinol.
The latter compound interacts with a S-adenosylmethionine through a bifunctional 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase and S-adenosylmethionine:2-DMK methyltransferase resulting in a s-adenosylhomocysteine, a hydrogen ion and a 6-methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol. The 6-methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol. interacts with a reduced acceptor, an oxygen molecule through a 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase resulting in the release of a water molecule, an oxidized electron acceptor and a 3-demethylubiquinol-8. The latter compound then interacts with a S-adenosylmethionine through a bifunctional 3-demethylubiquinone-8 3-O-methyltransferase and 2-octaprenyl-6-hydroxyphenol methylase resulting in a hydrogen ion, a S-adenosylhomocysteine and a ubiquinol 8.
PW000981MetabolicTCA cycle (ubiquinol-8)The TCA pathway is a catabolic pathway of aerobic respiration. It generates energy and reducing power. It is the first step in generating precursors for biosynthesis. When acetate is the carbon source, citrate synthase is rate-limiting for the TCA cycle. Respiration is an ATP-generating process in which compounds act as electron donors through a chain of electron transfer to electron acceptors. Aerobic respiration uses oxygen as the final acceptor. Anaerobic respiration uses several organic compounds as acceptors such as fumarate, nitrate and hydrogen. During the chain of electron transfer, protons (H+) are transported outside the cytoplasmic membrane, generating a proton motive force. Upon passage of protons back into the cytoplasm, the PMF energy is captured as ATP, catalyzed by a multisubunit ATPase.
The cycle can start from Acetyl-CoA interacting with Oxalacetic acid and water through a citrate synthase monomer resulting in a hydrogen ion, CoA and a Citric Acid. The latter compound is dehydrated by a Citrate hydro-lyase resulting in the release of water and a cis-Aconitic acid. This compound is then hydrated through a Citrate hydro-lyase resulting in a D-threo-Isocitric acid. This compound is decarboxylated by an NADP dependent Citrate dehydrogenase, resulting in a release of carbon dioxide and NADPH and Oxoglutaric acid. The oxoglutaric acid interacts with a Coenzyme A through a NAD driven 2-oxoglutarate dehydrogenase resulting in a release of carbon dioxide, an NADH and succinyl-CoA. The succinyl-CoA interacts with a phosphate and an ADP through a 2-oxoglutarate dehydrogenase resulting in a CoA, an ATP and Succinic Acid. Succinic acid interacts with a ubiquinone, in this case a ubiquinone 1 through a succinate:quinone oxidoreductase resulting in an ubiquinol, in this case a ubiquinol-1 and a fumaric acid.
The fumaric acid interacts with water through a fumarase hydratase resulting in a L-Malic acid.This compound can either interact with quinone through a malate:quinone oxidoreductase resulting in a release of hydroquinone and oxalacetic acid, or it can react with an NAD through a malate dehydrogenase resulting in a hydrogen ion, NADH and Oxalacetic acid.PW001008MetabolicSecondary Metabolites: Ubiquinol biosynthesis 2The biosynthesis of ubiquinol starts the interaction of 4-hydroxybenzoic acid interacting with an octaprenyl diphosphate. The former compound comes from the chorismate interacting with a chorismate lyase resulting in the release of a pyruvic acid and a 4-hydroxybenzoic acid. On the other hand, the latter compound, octaprenyl diphosphate is the result of a farnesyl pyrophosphate interacting with an isopentenyl pyrophosphate through an octaprenyl diphosphate synthase resulting in the release of a pyrophosphate and an octaprenyl diphosphate. The 4-hydroxybenzoic acid interacts with octaprenyl diphosphate through a 4-hydroxybenzoate octaprenyltransferase resulting in the release of a pyrophosphate and a 3-octaprenyl-4-hydroxybenzoate. The latter compound then interacts with a hydrogen ion through a 3-octaprenyl-4-hydroxybenzoate carboxy-lyase resulting in the release of a carbon dioxide and a 2-octaprenylphenol. The latter compound interacts with an oxygen molecule and a hydrogen ion through a NADPH driven 2-octaprenylphenol hydroxylase resulting in a NADP, a water molecule and a 2-octaprenyl-6-hydroxyphenol. The 2-octaprenyl-6-hydroxyphenol interacts with an S-adenosylmethionine through a bifunctional 3-demethylubiquinone-8 3-O-methyltransferase and 2-octaprenyl-6-hydroxyphenol methylase resulting in the release of a hydrogen ion, an s-adenosylhomocysteine and a 2-methoxy-6-(all-trans-octaprenyl)phenol. The latter compound then interacts with an oxygen molecule and a hydrogen ion through a NADPH driven 2-octaprenyl-6-methoxyphenol hydroxylase resulting in a NADP, a water molecule and a 2-methoxy-6-all trans-octaprenyl-2-methoxy-1,4-benzoquinol. The latter compound interacts with a S-adenosylmethionine through a bifunctional 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase and S-adenosylmethionine:2-DMK methyltransferase resulting in a s-adenosylhomocysteine, a hydrogen ion and a 6-methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol. The 6-methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol. interacts with a reduced acceptor, an oxygen molecule through a 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone hydroxylase resulting in the release of a water molecule, an oxidized electron acceptor and a 3-demethylubiquinol-8. The latter compound then interacts with a S-adenosylmethionine through a bifunctional 3-demethylubiquinone-8 3-O-methyltransferase and 2-octaprenyl-6-hydroxyphenol methylase resulting in a hydrogen ion, a S-adenosylhomocysteine and a ubiquinol 8.PW002036Metabolicubiquinol-8 biosynthesis (prokaryotic)PWY-6708Specdb::MsMs1308229Specdb::MsMs1308230Specdb::MsMs1308231Specdb::MsMs1422736Specdb::MsMs1422737Specdb::MsMs1422738HMDB122991123C0039017976CPD-9956Keseler, I. M., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A. G., Mackie, A., Paulsen, I., Gunsalus, R. P., Karp, P. D. (2011). "EcoCyc: a comprehensive database of Escherichia coli biology." Nucleic Acids Res 39:D583-D590.21097882Kanehisa, M., Goto, S., Sato, Y., Furumichi, M., Tanabe, M. (2012). "KEGG for integration and interpretation of large-scale molecular data sets." Nucleic Acids Res 40:D109-D114.22080510Pastore A, Giovamberardino GD, Bertini E, Tozzi G, Gaeta LM, Federici G, Piemonte F: Simultaneous determination of ubiquinol and ubiquinone in skeletal muscle of pediatric patients. Anal Biochem. 2005 Jul 15;342(2):352-5. Epub 2005 Mar 7.15989930Maneiro E, Lopez-Armada MJ, de Andres MC, Carames B, Martin MA, Bonilla A, Del Hoyo P, Galdo F, Arenas J, Blanco FJ: Effect of nitric oxide on mitochondrial respiratory activity of human articular chondrocytes. Ann Rheum Dis. 2005 Mar;64(3):388-95.15708893Nohl H, Gille L, Staniek K: Intracellular generation of reactive oxygen species by mitochondria. Biochem Pharmacol. 2005 Mar 1;69(5):719-23. Epub 2005 Jan 20.15710349NADH dehydrogenaseP00393DHNA_ECOLIndhhttp://ecmdb.ca/proteins/P00393.xmlD-lactate dehydrogenaseP06149DLD_ECOLIdldhttp://ecmdb.ca/proteins/P06149.xmlPyruvate dehydrogenase [cytochrome]P07003POXB_ECOLIpoxBhttp://ecmdb.ca/proteins/P07003.xmlSuccinate dehydrogenase iron-sulfur subunitP07014DHSB_ECOLIsdhBhttp://ecmdb.ca/proteins/P07014.xmlRespiratory nitrate reductase 1 alpha chainP09152NARG_ECOLInarGhttp://ecmdb.ca/proteins/P09152.xmlDihydroorotate dehydrogenaseP0A7E1PYRD_ECOLIpyrDhttp://ecmdb.ca/proteins/P0A7E1.xmlAnaerobic glycerol-3-phosphate dehydrogenase subunit CP0A996GLPC_ECOLIglpChttp://ecmdb.ca/proteins/P0A996.xmlAnaerobic glycerol-3-phosphate dehydrogenase subunit AP0A9C0GLPA_ECOLIglpAhttp://ecmdb.ca/proteins/P0A9C0.xmlFormate dehydrogenase, nitrate-inducible, iron-sulfur subunitP0AAJ3FDNH_ECOLIfdnHhttp://ecmdb.ca/proteins/P0AAJ3.xmlFormate dehydrogenase-O iron-sulfur subunitP0AAJ5FDOH_ECOLIfdoHhttp://ecmdb.ca/proteins/P0AAJ5.xmlFormate hydrogenlyase subunit 2P0AAK1HYCB_ECOLIhycBhttp://ecmdb.ca/proteins/P0AAK1.xmlCytochrome c-552P0ABK9NRFA_ECOLInrfAhttp://ecmdb.ca/proteins/P0ABK9.xmlSuccinate dehydrogenase flavoprotein subunitP0AC41DHSA_ECOLIsdhAhttp://ecmdb.ca/proteins/P0AC41.xmlSuccinate dehydrogenase hydrophobic membrane anchor subunitP0AC44DHSD_ECOLIsdhDhttp://ecmdb.ca/proteins/P0AC44.xmlHydrogenase-1 large chainP0ACD8MBHL_ECOLIhyaBhttp://ecmdb.ca/proteins/P0ACD8.xmlHydrogenase-2 large chainP0ACE0MBHM_ECOLIhybChttp://ecmdb.ca/proteins/P0ACE0.xmlProbable quinol monooxygenase ygiNP0ADU2YGIN_ECOLIygiNhttp://ecmdb.ca/proteins/P0ADU2.xmlFormate dehydrogenase, nitrate-inducible, cytochrome b556(fdn) subunitP0AEK7FDNI_ECOLIfdnIhttp://ecmdb.ca/proteins/P0AEK7.xmlFormate dehydrogenase, cytochrome b556(fdo) subunitP0AEL0FDOI_ECOLIfdoIhttp://ecmdb.ca/proteins/P0AEL0.xmlGlycolate oxidase subunit glcDP0AEP9GLCD_ECOLIglcDhttp://ecmdb.ca/proteins/P0AEP9.xmlRespiratory nitrate reductase 2 gamma chainP0AF32NARV_ECOLInarVhttp://ecmdb.ca/proteins/P0AF32.xmlNADH-quinone oxidoreductase subunit AP0AFC3NUOA_ECOLInuoAhttp://ecmdb.ca/proteins/P0AFC3.xmlNADH-quinone oxidoreductase subunit BP0AFC7NUOB_ECOLInuoBhttp://ecmdb.ca/proteins/P0AFC7.xmlNADH-quinone oxidoreductase subunit EP0AFD1NUOE_ECOLInuoEhttp://ecmdb.ca/proteins/P0AFD1.xmlNADH-quinone oxidoreductase subunit HP0AFD4NUOH_ECOLInuoHhttp://ecmdb.ca/proteins/P0AFD4.xmlNADH-quinone oxidoreductase subunit IP0AFD6NUOI_ECOLInuoIhttp://ecmdb.ca/proteins/P0AFD6.xmlNADH-quinone oxidoreductase subunit JP0AFE0NUOJ_ECOLInuoJhttp://ecmdb.ca/proteins/P0AFE0.xmlNADH-quinone oxidoreductase subunit KP0AFE4NUOK_ECOLInuoKhttp://ecmdb.ca/proteins/P0AFE4.xmlNADH-quinone oxidoreductase subunit MP0AFE8NUOM_ECOLInuoMhttp://ecmdb.ca/proteins/P0AFE8.xmlNADH-quinone oxidoreductase subunit NP0AFF0NUON_ECOLInuoNhttp://ecmdb.ca/proteins/P0AFF0.xmlL-aspartate oxidaseP10902NADB_ECOLInadBhttp://ecmdb.ca/proteins/P10902.xmlRespiratory nitrate reductase 1 beta chainP11349NARH_ECOLInarHhttp://ecmdb.ca/proteins/P11349.xmlRespiratory nitrate reductase 1 gamma chainP11350NARI_ECOLInarIhttp://ecmdb.ca/proteins/P11350.xmlAnaerobic glycerol-3-phosphate dehydrogenase subunit BP13033GLPB_ECOLIglpBhttp://ecmdb.ca/proteins/P13033.xmlAerobic glycerol-3-phosphate dehydrogenaseP13035GLPD_ECOLIglpDhttp://ecmdb.ca/proteins/P13035.xmlQuinoprotein glucose dehydrogenaseP15877DHG_ECOLIgcdhttp://ecmdb.ca/proteins/P15877.xmlFormate hydrogenlyase subunit 5P16431HYCE_ECOLIhycEhttp://ecmdb.ca/proteins/P16431.xmlFormate hydrogenlyase subunit 6P16432HYCF_ECOLIhycFhttp://ecmdb.ca/proteins/P16432.xmlFormate hydrogenlyase subunit 7P16433HYCG_ECOLIhycGhttp://ecmdb.ca/proteins/P16433.xml3-demethylubiquinone-9 3-methyltransferaseP17993UBIG_ECOLIubiGhttp://ecmdb.ca/proteins/P17993.xmlProbable nitrate reductase molybdenum cofactor assembly chaperone NarWP19317NARW_ECOLInarWhttp://ecmdb.ca/proteins/P19317.xmlRespiratory nitrate reductase 2 beta chainP19318NARY_ECOLInarYhttp://ecmdb.ca/proteins/P19318.xmlRespiratory nitrate reductase 2 alpha chainP19319NARZ_ECOLInarZhttp://ecmdb.ca/proteins/P19319.xmlFormate dehydrogenase, nitrate-inducible, major subunitP24183FDNG_ECOLIfdnGhttp://ecmdb.ca/proteins/P24183.xmlNADH-quinone oxidoreductase subunit FP31979NUOF_ECOLInuoFhttp://ecmdb.ca/proteins/P31979.xmlFormate dehydrogenase-O major subunitP32176FDOG_ECOLIfdoGhttp://ecmdb.ca/proteins/P32176.xmlL-lactate dehydrogenase [cytochrome]P33232LLDD_ECOLIlldDhttp://ecmdb.ca/proteins/P33232.xmlNADH-quinone oxidoreductase subunit C/DP33599NUOCD_ECOLInuoChttp://ecmdb.ca/proteins/P33599.xmlNADH-quinone oxidoreductase subunit GP33602NUOG_ECOLInuoGhttp://ecmdb.ca/proteins/P33602.xmlNADH-quinone oxidoreductase subunit LP33607NUOL_ECOLInuoLhttp://ecmdb.ca/proteins/P33607.xmlPeriplasmic nitrate reductaseP33937NAPA_ECOLInapAhttp://ecmdb.ca/proteins/P33937.xmlMalate:quinone oxidoreductaseP33940MQO_ECOLImqohttp://ecmdb.ca/proteins/P33940.xmlGlycolate oxidase iron-sulfur subunitP52074GLCF_ECOLIglcFhttp://ecmdb.ca/proteins/P52074.xmlSuccinate dehydrogenase cytochrome b556 subunitP69054DHSC_ECOLIsdhChttp://ecmdb.ca/proteins/P69054.xmlHydrogenase-1 small chainP69739MBHS_ECOLIhyaAhttp://ecmdb.ca/proteins/P69739.xmlHydrogenase-2 small chainP69741MBHT_ECOLIhybOhttp://ecmdb.ca/proteins/P69741.xmlFerredoxin-type protein napGP0AAL3NAPG_ECOLInapGhttp://ecmdb.ca/proteins/P0AAL3.xmlModulator of drug activity BP0AEY5MDAB_ECOLImdaBhttp://ecmdb.ca/proteins/P0AEY5.xmlUncharacterized protein ykgEP77252YKGE_ECOLIykgEhttp://ecmdb.ca/proteins/P77252.xmlFerredoxin-type protein napHP33934NAPH_ECOLInapHhttp://ecmdb.ca/proteins/P33934.xmlUbiquinol oxidase subunit 2P0ABJ1CYOA_ECOLIcyoAhttp://ecmdb.ca/proteins/P0ABJ1.xmlProbable Ni/Fe-hydrogenase 1 B-type cytochrome subunitP0AAM1CYBH_ECOLIhyaChttp://ecmdb.ca/proteins/P0AAM1.xmlUncharacterized protein ykgGP77433YKGG_ECOLIykgGhttp://ecmdb.ca/proteins/P77433.xmlGlycolate oxidase subunit glcEP52073GLCE_ECOLIglcEhttp://ecmdb.ca/proteins/P52073.xmlCytochrome o ubiquinol oxidase protein cyoDP0ABJ6CYOD_ECOLIcyoDhttp://ecmdb.ca/proteins/P0ABJ6.xmlThiol:disulfide interchange protein dsbAP0AEG4DSBA_ECOLIdsbAhttp://ecmdb.ca/proteins/P0AEG4.xmlHydrogenase-2 operon protein hybAP0AAJ8HYBA_ECOLIhybAhttp://ecmdb.ca/proteins/P0AAJ8.xmlUncharacterized electron transport protein ykgFP77536YKGF_ECOLIykgFhttp://ecmdb.ca/proteins/P77536.xmlCytochrome bd-II oxidase subunit 2P26458APPB_ECOLIappBhttp://ecmdb.ca/proteins/P26458.xmlProtein nrfDP32709NRFD_ECOLInrfDhttp://ecmdb.ca/proteins/P32709.xmlProbable Ni/Fe-hydrogenase 2 b-type cytochrome subunitP37180HYBB_ECOLIhybBhttp://ecmdb.ca/proteins/P37180.xmlCytochrome bd-II oxidase subunit 1P26459APPC_ECOLIappChttp://ecmdb.ca/proteins/P26459.xmlProtein nrfCP0AAK7NRFC_ECOLInrfChttp://ecmdb.ca/proteins/P0AAK7.xmlCytochrome d ubiquinol oxidase subunit 2P0ABK2CYDB_ECOLIcydBhttp://ecmdb.ca/proteins/P0ABK2.xmlCytochrome c-type protein nrfBP0ABL1NRFB_ECOLInrfBhttp://ecmdb.ca/proteins/P0ABL1.xmlSoluble aldose sugar dehydrogenase yliIP75804YLII_ECOLIyliIhttp://ecmdb.ca/proteins/P75804.xmlDiheme cytochrome c napBP0ABL3NAPB_ECOLInapBhttp://ecmdb.ca/proteins/P0ABL3.xmlUbiquinol oxidase subunit 1P0ABI8CYOB_ECOLIcyoBhttp://ecmdb.ca/proteins/P0ABI8.xmlFormate hydrogenlyase subunit 3P16429HYCC_ECOLIhycChttp://ecmdb.ca/proteins/P16429.xmlDisulfide bond formation protein BP0A6M2DSBB_ECOLIdsbBhttp://ecmdb.ca/proteins/P0A6M2.xmlCytochrome d ubiquinol oxidase subunit 1P0ABJ9CYDA_ECOLIcydAhttp://ecmdb.ca/proteins/P0ABJ9.xmlCytochrome c-type protein napCP0ABL5NAPC_ECOLInapChttp://ecmdb.ca/proteins/P0ABL5.xmlCytochrome o ubiquinol oxidase subunit 3P0ABJ3CYOC_ECOLIcyoChttp://ecmdb.ca/proteins/P0ABJ3.xmlNitrate reductase molybdenum cofactor assembly chaperone NarJP0AF26NARJ_ECOLInarJhttp://ecmdb.ca/proteins/P0AF26.xmlFormate hydrogenlyase subunit 4P16430HYCD_ECOLIhycDhttp://ecmdb.ca/proteins/P16430.xml2 Hydrogen ion + Hydrogen (gas) + Ubiquinone-8 > Ubiquinol-8 +2 Hydrogen ion2 Hydrogen ion + Oxygen + Ubiquinol-8 > Water + Ubiquinone-8 +2 Hydrogen ion2 Hydrogen ion + Nitrate + Ubiquinol-8 > Water + Nitrite + Ubiquinone-8 +2 Hydrogen ionUbiquinol-8 + Nitrate > Ubiquinone-8 + Water + NitriteGlycerol 3-phosphate + Ubiquinone-8 > Dihydroxyacetone phosphate + Ubiquinol-82 Hydrogen ion + Ubiquinone-8 + Formic acid > Ubiquinol-8 + Carbon dioxide + Hydrogen ionUbiquinone-8 + D-Glucose + Water > Ubiquinol-8 + Gluconic acid + Hydrogen ion4 Hydrogen ion + Oxygen + Ubiquinol-8 > Water + Ubiquinone-8 +4 Hydrogen ionUbiquinone-8 + Succinic acid > Fumaric acid + Ubiquinol-84 Hydrogen ion + NADH + Ubiquinone-8 > NAD + Ubiquinol-8 +3 Hydrogen ionGlycolic acid + Ubiquinone-8 > Glyoxylic acid + Ubiquinol-8L-Lactic acid + Ubiquinone-8 > Pyruvic acid + Ubiquinol-8Ubiquinone-8 + periplasmic protein disulfide isomerase I (reduced) > Ubiquinol-8 + periplasmic protein disulfide isomerase I (oxidized)3 Ubiquinol-8 + 2 Hydrogen ion + Nitrite >3 Ubiquinone-8 +2 Water + AmmoniumWater + Pyruvic acid + Ubiquinone-8 > Acetic acid + Carbon dioxide + Ubiquinol-84,5-Dihydroorotic acid + Ubiquinone-8 > Orotic acid + Ubiquinol-8Hydrogen ion + NADH + Ubiquinone-8 > NAD + Ubiquinol-8D-Lactic acid + Ubiquinone-8 > Pyruvic acid + Ubiquinol-8L-Malic acid + Ubiquinone-8 > Oxalacetic acid + Ubiquinol-82-Octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinol + S-Adenosylmethionine > S-Adenosylhomocysteine + Hydrogen ion + Ubiquinol-8L-Aspartic acid + Ubiquinone-8 > Hydrogen ion + Iminoaspartic acid + Ubiquinol-8Hydrogen ion + NADPH + Ubiquinone-8 > NADP + Ubiquinol-82 Oxygen + Ubiquinol-8 >2 Hydrogen ion +2 Superoxide anion + Ubiquinone-8Ubiquinol-8 + Acceptor <> Ubiquinone-1 + Reduced acceptorR02166Pyruvic acid + Ubiquinone-1 + Water <> Acetic acid + Ubiquinol-8 + Carbon dioxideR03145D-Glucose + Ubiquinone-1 <> Gluconolactone + Ubiquinol-8R066202-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone + S-Adenosylmethionine > Hydrogen ion + Ubiquinol-8 + S-AdenosylhomocysteineDHHB-METHYLTRANSFER-RXNUbiquinone-8 + Hydrogen ion > Ubiquinol-8RXN0-5309Ubiquinol-8 + Oxygen > Ubiquinone-8 + WaterNADH + Ubiquinone-1 <> NAD + Ubiquinol-8R02163 Succinic acid + Ubiquinone-8 > Fumaric acid + Ubiquinol 8 + Ubiquinol-8PW_R0037513-demethylubiquinol-8 + S-adenosyl-L-methionine > Hydrogen ion + S-Adenosylhomocysteine + Ubiquinol 8 + Ubiquinol-8PW_R0037232-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone + S-adenosyl-L-methionine > S-Adenosylhomocysteine + Ubiquinol-8 + Hydrogen ionPW_R005948D-Glucose + Ubiquinone-1 <> Gluconolactone + Ubiquinol-8Ubiquinol-8 + Acceptor <> Ubiquinone-1 + Reduced acceptorNADH + Ubiquinone-1 <> NAD + Ubiquinol-8Pyruvic acid + Ubiquinone-1 + Water <> Acetic acid + Ubiquinol-8 + Carbon dioxideD-Glucose + Ubiquinone-1 <> Gluconolactone + Ubiquinol-8Ubiquinol-8 + Acceptor <> Ubiquinone-1 + Reduced acceptorUbiquinol-8 + Acceptor <> Ubiquinone-1 + Reduced acceptorUbiquinol-8 + Acceptor <> Ubiquinone-1 + Reduced acceptorUbiquinol-8 + Acceptor <> Ubiquinone-1 + Reduced acceptorUbiquinol-8 + Acceptor <> Ubiquinone-1 + Reduced acceptorUbiquinol-8 + Acceptor <> Ubiquinone-1 + Reduced acceptorPyruvic acid + Ubiquinone-1 + Water <> Acetic acid + Ubiquinol-8 + Carbon dioxide