Record Information
Version2.0
Creation Date2012-05-31 10:24:07 -0600
Update Date2015-06-03 15:53:24 -0600
Secondary Accession Numbers
  • ECMDB00223
Identification
Name:Oxalacetic acid
Description:Oxaloacetic acid, also known as oxosuccinic acid or oxalacetic acid, is a four-carbon dicarboxylic acid appearing as an intermediate of the citric acid cycle. In vivo, oxaloacetate (the ionized form of oxaloacetic acid) is formed by the oxidation of L-malate, catalyzed by malate dehydrogenase, and reacts with Acetyl-CoA to form citrate, catalyzed by citrate synthase.(wikipedia) A class of ketodicarboxylic acids derived from oxalic acid. Oxaloacetic acid is an intermediate in the citric acid cycle and is converted to aspartic acidD by a transamination reaction.
Structure
Thumb
Synonyms:
  • 2-Ketosuccinate
  • 2-Ketosuccinic acid
  • 2-Oxobutanedioate
  • 2-Oxobutanedioic acid
  • 2-Oxosuccinate
  • 2-Oxosuccinic acid
  • A-Ketosuccinate
  • A-Ketosuccinic acid
  • Alpha-Ketosuccinate
  • Alpha-Ketosuccinic acid
  • Keto-oxaloacetate
  • Keto-oxaloacetic acid
  • Ketosuccinate
  • Ketosuccinic acid
  • OAA
  • Oxalacetate
  • Oxalacetic acid
  • Oxaloacetate
  • Oxaloacetic acid
  • Oxaloethanoate
  • Oxaloethanoic acid
  • Oxosuccinate
  • Oxosuccinic acid
  • α-Ketosuccinate
  • α-Ketosuccinic acid
Chemical Formula:C4H4O5
Weight:Average: 132.0716
Monoisotopic: 132.005873238
InChI Key:KHPXUQMNIQBQEV-UHFFFAOYSA-N
InChI:InChI=1S/C4H4O5/c5-2(4(8)9)1-3(6)7/h1H2,(H,6,7)(H,8,9)
CAS number:328-42-7
IUPAC Name:2-oxobutanedioic acid
Traditional IUPAC Name:oxalacetate
SMILES:OC(=O)CC(=O)C(O)=O
Chemical Taxonomy
DescriptionThis compound belongs to the class of chemical entities known as short-chain keto acids and derivatives. These are keto acids with an alkyl chain the contains less than 6 carbon atoms.
KingdomChemical entities
Super ClassOrganic compounds
ClassOrganic acids and derivatives
Sub ClassKeto acids and derivatives
Direct ParentShort-chain keto acids and derivatives
Alternative Parents
Substituents
  • Beta-keto acid
  • Short-chain keto acid
  • Alpha-keto acid
  • Beta-hydroxy ketone
  • Dicarboxylic acid or derivatives
  • 1,3-dicarbonyl compound
  • Alpha-hydroxy ketone
  • Ketone
  • Carboxylic acid
  • Carboxylic acid derivative
  • Carbonyl group
  • Organooxygen compound
  • Organic oxide
  • Organic oxygen compound
  • Hydrocarbon derivative
  • Aliphatic acyclic compound
Molecular FrameworkAliphatic acyclic compounds
External Descriptors
Physical Properties
State:Solid
Charge:-2
Melting point:161 °C
Experimental Properties:
PropertyValueSource
Water Solubility:134 mg/mL [HMP experimental]PhysProp
Predicted Properties
PropertyValueSource
Water Solubility57.1 mg/mLALOGPS
logP-0.68ALOGPS
logP-0.042ChemAxon
logS-0.36ALOGPS
pKa (Strongest Acidic)2.41ChemAxon
pKa (Strongest Basic)-9.9ChemAxon
Physiological Charge-2ChemAxon
Hydrogen Acceptor Count5ChemAxon
Hydrogen Donor Count2ChemAxon
Polar Surface Area91.67 Å2ChemAxon
Rotatable Bond Count3ChemAxon
Refractivity24.33 m3·mol-1ChemAxon
Polarizability10.06 Å3ChemAxon
Number of Rings0ChemAxon
Bioavailability1ChemAxon
Rule of FiveYesChemAxon
Ghose FilterYesChemAxon
Veber's RuleYesChemAxon
MDDR-like RuleYesChemAxon
Biological Properties
Cellular Locations:Cytoplasm
Reactions:
Citric acid <> Acetic acid + Oxalacetic acid
Tartaric acid <> Water + Oxalacetic acid
Water + Oxalacetic acid + Propionyl-CoA <> Methylcitric acid + Coenzyme A + Hydrogen ion + (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate
Acetyl-CoA + Water + Oxalacetic acid <> Citric acid + Coenzyme A + Hydrogen ion
alpha-Ketoglutarate + L-Aspartic acid <> L-Glutamate + Oxalacetic acid
Hydrogen ion + Oxalacetic acid > Carbon dioxide + Pyruvic acid
L-Malic acid + Ubiquinone-8 > Oxalacetic acid + Ubiquinol-8
L-Malic acid + Menaquinone 8 > Menaquinol 8 + Oxalacetic acid
L-Malic acid + NAD <> Hydrogen ion + NADH + Oxalacetic acid
Adenosine triphosphate + Oxalacetic acid <> ADP + Carbon dioxide + Phosphoenolpyruvic acid
Carbon dioxide + Water + Phosphoenolpyruvic acid <> Hydrogen ion + Oxalacetic acid + Phosphate + Hydrogen carbonate
D-tartrate > Water + Oxalacetic acid
Tartaric acid <> Oxalacetic acid + Water
Adenosine triphosphate + Oxalacetic acid <> ADP + Phosphoenolpyruvic acid + Carbon dioxide
L-Malic acid + NAD <> Oxalacetic acid + NADH + Hydrogen ion
Phosphate + Oxalacetic acid <> Water + Phosphoenolpyruvic acid + Carbon dioxide
Citric acid + Coenzyme A <> Acetyl-CoA + Water + Oxalacetic acid
L-Aspartic acid + alpha-Ketoglutarate <> Oxalacetic acid + L-Glutamate
L-Aspartic acid + Water + Oxygen <> Oxalacetic acid + Ammonia + Hydrogen peroxide
Citric acid <> Acetic acid + Oxalacetic acid
Methylcitric acid + Coenzyme A <> Propionyl-CoA + Oxalacetic acid + Water
L-Malic acid + FAD <> FADH2 + Oxalacetic acid
Oxalacetic acid + L-Arogenate <> L-Aspartic acid + Prephenate
Oxalacetic acid + Water + Propionyl-CoA <> Hydrogen ion + Methylcitric acid + Coenzyme A
L-Aspartic acid + Oxoglutaric acid <> Oxalacetic acid + L-Glutamate
Citric acid > Acetic acid + Oxalacetic acid
Oxalacetic acid + Acetyl-CoA + Water <> Hydrogen ion + Citric acid + Coenzyme A
D-tartrate Water + Oxalacetic acid
Tartaric acid <> Oxalacetic acid + Water
L-Malic acid + NAD <> Hydrogen ion + Oxalacetic acid + NADH
L-Malic acid + a quinone > Oxalacetic acid + a quinol
L-Malic acid + Oxygen <> Oxalacetic acid + Hydrogen peroxide
Oxalacetic acid enol-oxaloacetate
Hydrogen ion + Oxalacetic acid > Pyruvic acid + Carbon dioxide
Phosphate + Oxalacetic acid <> Phosphoenolpyruvic acid + Hydrogen carbonate
Oxalacetic acid + Adenosine triphosphate > Carbon dioxide + Phosphoenolpyruvic acid + ADP
Pyridoxamine + Oxalacetic acid <> Pyridoxal + L-Aspartic acid
L-Aspartic acid + Oxoglutaric acid > Oxalacetic acid + L-Glutamate
Inorganic phosphate + Oxalacetic acid > Water + Phosphoenolpyruvic acid + Carbonic acid
Citric acid > Acetic acid + Oxalacetic acid
Acetyl-CoA + Water + Oxalacetic acid > Citric acid + CoA
Citric acid > Acetic acid + Oxalacetic acid
(3S)-Citryl-CoA > Acetyl-CoA + Oxalacetic acid
L-Malic acid + NAD > Oxalacetic acid + NADH
L-Malic acid + a quinone > Oxalacetic acid + reduced quinone
Adenosine triphosphate + Oxalacetic acid > ADP + Phosphoenolpyruvic acid + Carbon dioxide
Propionyl-CoA + Water + Oxalacetic acid > (2R,3S)-2-Hydroxybutane-1,2,3-tricarboxylate + CoA
Tartaric acid > Oxalacetic acid + Water
Tartaric acid > Oxalacetic acid + Water
(3S)-Citryl-CoA <> Acetyl-CoA + Oxalacetic acid
L-Malic acid + NAD + Oxalacetic acid <> Pyruvic acid + Carbon dioxide + NADH
L-Malic acid + Quinone <> Oxalacetic acid + Hydroquinone
L-Malic acid + NADP + Oxalacetic acid <> Pyruvic acid + Carbon dioxide + NADPH
Acetyl-CoA + Water + Oxalacetic acid > Citric acid + Coenzyme A
L-Malic acid + NAD + L-Malic acid <> Oxalacetic acid + NADH + Hydrogen ion
Adenosine triphosphate + Pyruvic acid + Hydrogen carbonate > Adenosine diphosphate + Phosphate + Oxalacetic acid + ADP
Oxalacetic acid + Water + Acetyl-CoA > Citric acid + Coenzyme A + Hydrogen ion
L-Malic acid + NAD + L-Malic acid > Oxalacetic acid + NADH + Hydrogen ion
L-Malic acid + Quinone + L-Malic acid > Oxalacetic acid + Hydroquinone
L-Aspartic acid + Oxoglutaric acid + L-Aspartic acid > Oxalacetic acid + L-Glutamic acid + L-Glutamate
L-Glutamic acid + Oxalacetic acid + L-Glutamate > L-Aspartic acid + Oxoglutaric acid + L-Aspartic acid
L-Aspartic acid + Water + Oxygen + L-Aspartic acid > Oxalacetic acid + Ammonia + Hydrogen peroxide
Oxalacetic acid + Adenosine triphosphate > Adenosine diphosphate + Carbon dioxide + Phosphoenolpyruvic acid + ADP
Propionyl-CoA + Water + Oxalacetic acid + Propionyl-CoA > Coenzyme A + Hydrogen ion + 2-Methylcitric acid + Methylcitric acid
Tartaric acid > Oxalacetic acid + Water
More...

SMPDB Pathways:
Asparagine biosynthesisPW000813 Pw000813Pw000813 greyscalePw000813 simple
Aspartate metabolismPW000787 Pw000787Pw000787 greyscalePw000787 simple
Gluconeogenesis from L-malic acidPW000819 Pw000819Pw000819 greyscalePw000819 simple
L-glutamate metabolismPW000789 Pw000789Pw000789 greyscalePw000789 simple
Phenylalanine metabolismPW000921 Pw000921Pw000921 greyscalePw000921 simple
Propanoate metabolismPW000940 Pw000940Pw000940 greyscalePw000940 simple
Secondary Metabolites: Glyoxylate cyclePW000967 Pw000967Pw000967 greyscalePw000967 simple
TCA cyclePW000779 Pw000779Pw000779 greyscalePw000779 simple
TCA cycle (ubiquinol-0)PW002023 Pw002023Pw002023 greyscalePw002023 simple
TCA cycle (ubiquinol-10)PW001010 Pw001010Pw001010 greyscalePw001010 simple
TCA cycle (ubiquinol-2)PW001002 Pw001002Pw001002 greyscalePw001002 simple
TCA cycle (ubiquinol-3)PW001003 Pw001003Pw001003 greyscalePw001003 simple
TCA cycle (ubiquinol-4)PW001004 Pw001004Pw001004 greyscalePw001004 simple
TCA cycle (ubiquinol-5)PW001005 Pw001005Pw001005 greyscalePw001005 simple
TCA cycle (ubiquinol-6)PW001006 Pw001006Pw001006 greyscalePw001006 simple
TCA cycle (ubiquinol-7)PW001007 Pw001007Pw001007 greyscalePw001007 simple
TCA cycle (ubiquinol-8)PW001008 Pw001008Pw001008 greyscalePw001008 simple
TCA cycle (ubiquinol-9)PW001009 Pw001009Pw001009 greyscalePw001009 simple
glycolate and glyoxylate degradation IIPW002021 Pw002021Pw002021 greyscalePw002021 simple
threonine biosynthesisPW000817 Pw000817Pw000817 greyscalePw000817 simple
KEGG Pathways:
  • Alanine, aspartate and glutamate metabolism ec00250
  • Arginine and proline metabolism ec00330
  • Benzoate degradation via hydroxylation ec00362
  • Carbon fixation in photosynthetic organisms ec00710
  • Citrate cycle (TCA cycle) ec00020
  • Cysteine and methionine metabolism ec00270
  • Glycolysis / Gluconeogenesis ec00010
  • Glyoxylate and dicarboxylate metabolism ec00630
  • Isoquinoline alkaloid biosynthesis ec00950
  • Metabolic pathways eco01100
  • Methane metabolism ec00680
  • Microbial metabolism in diverse environments ec01120
  • Novobiocin biosynthesis ec00401
  • Phenylalanine metabolism ec00360
  • Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
  • Propanoate metabolism ec00640
  • Pyruvate metabolism ec00620
  • Reductive carboxylate cycle (CO2 fixation) ec00720
  • Tropane, piperidine and pyridine alkaloid biosynthesis ec00960
  • Two-component system ec02020
  • Tyrosine metabolism ec00350
EcoCyc Pathways:
Concentrations
ConcentrationStrainMediaGrowth StatusGrowth SystemTemperatureDetails
30± 1 uMBW25113M9 Minimal Media, 4 g/L GlucoseMid-Log PhaseBioreactor, pH controlled, O2 controlled, dilution rate: 0.2/h37 oCPMID: 15158257
Find out more about how we convert literature concentrations.
Spectra
Spectra:
Spectrum TypeDescriptionSplash Key
GC-MSGC-MS Spectrum - GC-EI-TOF (Pegasus III TOF-MS system, Leco; GC 6890, Agilent Technologies)splash10-00dr-4900000000-9d943d40beaca3c602a1View in MoNA
GC-MSGC-MS Spectrum - GC-MS (1 MEOX; 2 TMS)splash10-007a-9210000000-020f60717e2ea79d1ccdView in MoNA
GC-MSGC-MS Spectrum - GC-MS (1 MEOX; 2 TMS)splash10-000b-9540000000-a53f674cc98960834f88View in MoNA
GC-MSGC-MS Spectrum - GC-MS (1 MEOX; 3 TMS)splash10-001a-8940000000-40b790e06141d7180938View in MoNA
GC-MSGC-MS Spectrum - GC-MSNot Available
GC-MSGC-MS Spectrum - GC-EI-TOFsplash10-0002-4930000000-7e995327b0b9c1af0914View in MoNA
LC-MS/MSLC-MS/MS Spectrum - Quattro_QQQ 10V, Negative (Annotated)splash10-000i-9000000000-0be675f3aa3e973393b7View in MoNA
LC-MS/MSLC-MS/MS Spectrum - Quattro_QQQ 25V, Negative (Annotated)splash10-000f-9000000000-4fba97fcd7b0f2215f75View in MoNA
LC-MS/MSLC-MS/MS Spectrum - Quattro_QQQ 40V, Negative (Annotated)splash10-0006-9000000000-9b1f4171aee6283a3cbdView in MoNA
LC-MS/MSLC-MS/MS Spectrum - LC-ESI-TOF , negativesplash10-014i-0900000000-7c598fd9c78acb94dacdView in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 10V, Positivesplash10-014i-3900000000-6f4e965a3f513bf8db13View in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 20V, Positivesplash10-014i-9600000000-8ae7abf60057088e14dbView in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 40V, Positivesplash10-01bc-9100000000-5724c215c46a4f568140View in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 10V, Negativesplash10-0019-8900000000-829898d0849b1fbf1c28View in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 20V, Negativesplash10-000i-9100000000-5a5ff8fb7cb52eebf0c8View in MoNA
Predicted LC-MS/MSPredicted LC-MS/MS Spectrum - 40V, Negativesplash10-0006-9000000000-657d1384d478ddc33b6dView in MoNA
MSMass Spectrum (Electron Ionization)splash10-0006-9000000000-f371299a07d43c23ff1dView in MoNA
1D NMR1H NMR SpectrumNot Available
2D NMR[1H,13C] 2D NMR SpectrumNot Available
References
References:
  • Allen RH, Stabler SP, Savage DG, Lindenbaum J: Elevation of 2-methylcitric acid I and II levels in serum, urine, and cerebrospinal fluid of patients with cobalamin deficiency. Metabolism. 1993 Aug;42(8):978-88. Pubmed: 8345822
  • Dworzak E, Grunicke H, Berger H, Jarosch E, Haas H, Hopfel I: [Pyruvate dehydrogenase deficiency in a child with persistent lactic acidosis] J Clin Chem Clin Biochem. 1985 Jun;23(6):323-9. Pubmed: 3926941
  • Efimov AS, Gulyi MF, Shcherbak AV, Dzvonkevich ND: [Levels of Krebs cycle metabolites in the blood and urine of patients with diabetes mellitus] Probl Endokrinol (Mosk). 1983 Mar-Apr;29(2):10-4. Pubmed: 6856592
  • el-Sharabasy MM: Observations on calcium oxalate stone formers. Br J Urol. 1992 Nov;70(5):474-7. Pubmed: 1361403
  • Esenmo E, Chandramouli V, Schumann WC, Kumaran K, Wahren J, Landau BR: Use of 14CO2 in estimating rates of hepatic gluconeogenesis. Am J Physiol. 1992 Jul;263(1 Pt 1):E36-41. Pubmed: 1322046
  • Kanehisa, M., Goto, S., Sato, Y., Furumichi, M., Tanabe, M. (2012). "KEGG for integration and interpretation of large-scale molecular data sets." Nucleic Acids Res 40:D109-D114. Pubmed: 22080510
  • Keseler, I. M., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A. G., Mackie, A., Paulsen, I., Gunsalus, R. P., Karp, P. D. (2011). "EcoCyc: a comprehensive database of Escherichia coli biology." Nucleic Acids Res 39:D583-D590. Pubmed: 21097882
  • Koike K, Koike M: Fluorescent analysis of alpha-keto acids in serum and urine by high-performance liquid chromatography. Anal Biochem. 1984 Sep;141(2):481-7. Pubmed: 6437276
  • Olubuyide IO, Festing MF, Chapman C, Higginson J, Whicher JT: Discriminant analysis of biochemical parameters in liver disease. Trop Gastroenterol. 1997 Jan-Mar;18(1):15-9. Pubmed: 9197166
  • Peng, L., Arauzo-Bravo, M. J., Shimizu, K. (2004). "Metabolic flux analysis for a ppc mutant Escherichia coli based on 13C-labelling experiments together with enzyme activity assays and intracellular metabolite measurements." FEMS Microbiol Lett 235:17-23. Pubmed: 15158257
  • Petrarulo M, Facchini P, Cerelli E, Marangella M, Linari F: Citrate in urine determined with a new citrate lyase method. Clin Chem. 1995 Oct;41(10):1518-21. Pubmed: 7586527
  • Rabinovich PD, Miliushkin PV: [Content of biological oxidation metabolites in the blood and urine of peptic ulcer patients] Vopr Med Khim. 1979 Nov-Dec;25(6):755-8. Pubmed: 516538
  • Schauenstein E, Kronberger L, Schaur RJ, Fink E, Georgiopulos E: [Malate and oxaloacetate levels in whole blood of patients with and without malignant tumor diseases] Wien Klin Wochenschr. 1973 Jun 29;85(26):478-82. Pubmed: 4717666
  • Sperl W, Maurer H, Dworschak E, Hopfel I, Hammerer I: [Lactic acid acidosis with mitochondrial myopathy due to a pyruvate dehydrogenase deficiency] Padiatr Padol. 1985;20(1):55-67. Pubmed: 3919358
  • Sweatman BC, Farrant RD, Holmes E, Ghauri FY, Nicholson JK, Lindon JC: 600 MHz 1H-NMR spectroscopy of human cerebrospinal fluid: effects of sample manipulation and assignment of resonances. J Pharm Biomed Anal. 1993 Aug;11(8):651-64. Pubmed: 8257730
  • van der Werf, M. J., Overkamp, K. M., Muilwijk, B., Coulier, L., Hankemeier, T. (2007). "Microbial metabolomics: toward a platform with full metabolome coverage." Anal Biochem 370:17-25. Pubmed: 17765195
  • Winder, C. L., Dunn, W. B., Schuler, S., Broadhurst, D., Jarvis, R., Stephens, G. M., Goodacre, R. (2008). "Global metabolic profiling of Escherichia coli cultures: an evaluation of methods for quenching and extraction of intracellular metabolites." Anal Chem 80:2939-2948. Pubmed: 18331064
  • Wong LT, Davidson AG, Applegarth DA, Dimmick JE, Norman MG, Toone JR, Pirie G, Wong J: Biochemical and histologic pathology in an infant with cross-reacting material (negative) pyruvate carboxylase deficiency. Pediatr Res. 1986 Mar;20(3):274-9. Pubmed: 3085060
  • Zupke C, Sinskey AJ, Stephanopoulos G: Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl Microbiol Biotechnol. 1995 Dec;44(1-2):27-36. Pubmed: 8579834
Synthesis Reference:Heidelberger, Charles; Hurlbert, Robert B. The synthesis of oxalacetic acid-I-C14 and orotic acid-6-C14. Journal of the American Chemical Society (1950), 72 4704-6.
Material Safety Data Sheet (MSDS)Not Available
External Links:
ResourceLink
CHEBI ID16452
HMDB IDHMDB00223
Pubchem Compound ID970
Kegg IDC00036
ChemSpider ID945
WikipediaOxalacetic acid
BioCyc IDOXALACETIC_ACID
EcoCyc IDOXALACETIC_ACID
Ligand ExpoOAA

Enzymes

General function:
Involved in transferase activity
Specific function:
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
Gene Name:
aspC
Uniprot ID:
P00509
Molecular weight:
43573
Reactions
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.
General function:
Involved in phosphoenolpyruvate carboxylase activity
Specific function:
Through the carboxylation of phosphoenolpyruvate (PEP) it forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle
Gene Name:
ppc
Uniprot ID:
P00864
Molecular weight:
99062
Reactions
Phosphate + oxaloacetate = H(2)O + phosphoenolpyruvate + HCO(3)(-).
General function:
Involved in transferase activity
Specific function:
An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate
Gene Name:
tyrB
Uniprot ID:
P04693
Molecular weight:
43537
Reactions
An aromatic amino acid + 2-oxoglutarate = an aromatic oxo acid + L-glutamate.
General function:
Involved in lyase activity
Specific function:
(R,R)-tartrate = oxaloacetate + H(2)O
Gene Name:
ttdA
Uniprot ID:
P05847
Molecular weight:
32733
Reactions
(R,R)-tartrate = oxaloacetate + H(2)O.
General function:
Involved in catalytic activity
Specific function:
4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate
Gene Name:
eda
Uniprot ID:
P0A955
Molecular weight:
22284
Reactions
4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.
2-dehydro-3-deoxy-D-gluconate 6-phosphate = pyruvate + D-glyceraldehyde 3-phosphate.
General function:
Involved in carbon-carbon lyase activity
Specific function:
Represents a citryl-ACP lyase
Gene Name:
citE
Uniprot ID:
P0A9I1
Molecular weight:
33109
Reactions
Citrate = acetate + oxaloacetate.
(3S)-citryl-CoA = acetyl-CoA + oxaloacetate.
General function:
Involved in transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
Specific function:
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
Gene Name:
gltA
Uniprot ID:
P0ABH7
Molecular weight:
48015
Reactions
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA.
General function:
Involved in hydro-lyase activity
Specific function:
(R,R)-tartrate = oxaloacetate + H(2)O
Gene Name:
ttdB
Uniprot ID:
P0AC35
Molecular weight:
22679
Reactions
(R,R)-tartrate = oxaloacetate + H(2)O.
General function:
Involved in electron carrier activity
Specific function:
Catalyzes the oxidation of L-aspartate to iminoaspartate
Gene Name:
nadB
Uniprot ID:
P10902
Molecular weight:
60337
Reactions
L-aspartate + O(2) = iminosuccinate + H(2)O(2).
General function:
Involved in lyase activity
Specific function:
It functions in the generation of fumarate for use as an anaerobic electron acceptor
Gene Name:
fumB
Uniprot ID:
P14407
Molecular weight:
60105
Reactions
(S)-malate = fumarate + H(2)O.
General function:
Involved in phosphoenolpyruvate carboxykinase (ATP) activity
Specific function:
ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
Gene Name:
pckA
Uniprot ID:
P22259
Molecular weight:
59643
Reactions
ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2).
General function:
Involved in transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
Specific function:
Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity
Gene Name:
prpC
Uniprot ID:
P31660
Molecular weight:
43102
Reactions
Propanoyl-CoA + H(2)O + oxaloacetate = (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA.
General function:
Involved in malate dehydrogenase (quinone) activity
Specific function:
(S)-malate + a quinone = oxaloacetate + reduced quinone
Gene Name:
mqo
Uniprot ID:
P33940
Molecular weight:
60229
Reactions
(S)-malate + a quinone = oxaloacetate + reduced quinone.
General function:
Involved in oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Specific function:
Catalyzes the reversible oxidation of malate to oxaloacetate
Gene Name:
mdh
Uniprot ID:
P61889
Molecular weight:
32337
Reactions
(S)-malate + NAD(+) = oxaloacetate + NADH.
General function:
Energy production and conversion
Specific function:
Covalent carrier of the coenzyme of citrate lyase
Gene Name:
citD
Uniprot ID:
P69330
Molecular weight:
10689
General function:
Involved in citrate CoA-transferase activity
Specific function:
Represents a citrate:acetyl-ACP transferase
Gene Name:
citF
Uniprot ID:
P75726
Molecular weight:
55172
Reactions
Citrate = acetate + oxaloacetate.
Acetyl-CoA + citrate = acetate + (3S)-citryl-CoA.
General function:
Involved in prosthetic group biosynthetic process
Specific function:
Transfers 2-(5''-triphosphoribosyl)-3'- dephosphocoenzyme-A on a serine residue to the apo-acyl carrier protein (gamma chain) of the citrate lyase to yield holo-acyl carrier protein
Gene Name:
citX
Uniprot ID:
P0A6G5
Molecular weight:
20270
Reactions
2'-(5-triphosphoribosyl)-3'-dephospho-CoA + citrate lyase apo-[acyl-carrier-protein] = citrate lyase holo-[acyl-carrier-protein] + diphosphate.